Pomaznoy, M., R. Kuan, M. Lindvall, J. G. Burel, G. Seumois, P. Vijayanand, R. Taplitz, R. H. Gilman, M. Saito, D. M. Lewinsohn, A. Sette, B. Peters, and C. S. Lindestam Arlehamn. 2020. Quantitative and qualitative perturbations of CD8+ MAITs in healthy Mycobacterium tuberculosis–infected individuals. ImmunoHorizons 4: 292–307; DOI: https://doi.org/10.4049/immunohorizons.2000031.
The graphs in Fig. 5F were inadvertently duplicated from Fig. 6 in the article as originally published. The corrected Fig. 5 is shown below. The figure legend was correct as published and is shown below for reference. The figure has been corrected in the online article.
![FIGURE 5. Gene expression profile and M. tuberculosis–specific signature of MR1tet− MAITs compared with memory CD8 T cells and MR1tet+ MAITs. / (A) PCA plot illustrating differences between memory CD8 T cells and MR1tet− MAITs and between LTBI and TB neg individuals. (B, D, and E) Volcano plots obtained from the DEseq2 analysis showing log2 fold change versus −log10 p value. The differentially expressed genes are represented in black [adjusted p value <0.01 (B and E) and p < 0.05 (D), absolute log2 fold change >1 are indicated by dotted lines]. (B) MR1tet− cells compared with memory CD8 T cells. (C) Venn-diagram showing overlap between the 226-gene signature identified in Fig. 4B and the signature in Fig. 5B, based on hypergeometric distribution test (considering the 18,315 transcripts detected within memory CD8 T cells as the total number of genes). (D) MR1tet− cells comparing individuals with LTBI versus TB neg. (E) Volcano plot comparing MR1tet− cells with MR1tet+ cells. (F) CCR1, CXCR4, CXCR6, TIGIT, IL-7R, and ABCB1 expression at the mRNA (upper panels: gene expression values in counts normalized by sequencing depth calculated by the DEseq2 package) and protein (lower panels: protein expression as percent frequency of subset) levels in memory CD8 T cells and MR1tet− MAITs. Gene expression data were derived from memory CD8 T cells from 17 individuals and MR1tet− cells (n individuals = 12) using an Illumina sequencing platform. Protein expression data were derived from memory CD8 T cells from 20 individuals and MR1tet− cells (n individuals = 7) using flow cytometry. Median ± interquartile range is shown. Two-tailed Mann–Whitney U test.](https://aai.silverchair-cdn.com/aai/content_public/journal/immunohorizons/4/7/10.4049_immunohorizons.2000064/4/m_ih2000064f5.jpeg?Expires=1735758743&Signature=NSuBtntemlUE0Pnhrz8LkwdOAFCU-tgpd6Y5L0yUomGamIoOCVq6xD0Bfb4rTKyczyNPfWc6nU3-d2FNyfd0SJGTkKA7cTMmsgm3evUWki1gpuT0BSrGdBq-fJmB~3hS67j2dpN5XusaqOaXEkdShZkPEBqi9aD5SXh1KA6TzgqIPeBKjlAd-VC8qnvJ8CdOVjWDJq~SBtXeacdGsCG7aWvz0Fw3L4vZVl190~NivITG1ycEjF7QpVrTa-qT5vlFQ7J54zWDe~AHKKneakfAYuq6kJp87319YiY0v4HVF3ITqD0xxh51W8ZwuLNx0yWbKFcola5ENFJFNqvpDnE1WA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gene expression profile and M. tuberculosis–specific signature of MR1tet− MAITs compared with memory CD8 T cells and MR1tet+ MAITs.
(A) PCA plot illustrating differences between memory CD8 T cells and MR1tet− MAITs and between LTBI and TB neg individuals. (B, D, and E) Volcano plots obtained from the DEseq2 analysis showing log2 fold change versus −log10 p value. The differentially expressed genes are represented in black [adjusted p value <0.01 (B and E) and p < 0.05 (D), absolute log2 fold change >1 are indicated by dotted lines]. (B) MR1tet− cells compared with memory CD8 T cells. (C) Venn-diagram showing overlap between the 226-gene signature identified in Fig. 4B and the signature in Fig. 5B, based on hypergeometric distribution test (considering the 18,315 transcripts detected within memory CD8 T cells as the total number of genes). (D) MR1tet− cells comparing individuals with LTBI versus TB neg. (E) Volcano plot comparing MR1tet− cells with MR1tet+ cells. (F) CCR1, CXCR4, CXCR6, TIGIT, IL-7R, and ABCB1 expression at the mRNA (upper panels: gene expression values in counts normalized by sequencing depth calculated by the DEseq2 package) and protein (lower panels: protein expression as percent frequency of subset) levels in memory CD8 T cells and MR1tet− MAITs. Gene expression data were derived from memory CD8 T cells from 17 individuals and MR1tet− cells (n individuals = 12) using an Illumina sequencing platform. Protein expression data were derived from memory CD8 T cells from 20 individuals and MR1tet− cells (n individuals = 7) using flow cytometry. Median ± interquartile range is shown. Two-tailed Mann–Whitney U test.
Gene expression profile and M. tuberculosis–specific signature of MR1tet− MAITs compared with memory CD8 T cells and MR1tet+ MAITs.
(A) PCA plot illustrating differences between memory CD8 T cells and MR1tet− MAITs and between LTBI and TB neg individuals. (B, D, and E) Volcano plots obtained from the DEseq2 analysis showing log2 fold change versus −log10 p value. The differentially expressed genes are represented in black [adjusted p value <0.01 (B and E) and p < 0.05 (D), absolute log2 fold change >1 are indicated by dotted lines]. (B) MR1tet− cells compared with memory CD8 T cells. (C) Venn-diagram showing overlap between the 226-gene signature identified in Fig. 4B and the signature in Fig. 5B, based on hypergeometric distribution test (considering the 18,315 transcripts detected within memory CD8 T cells as the total number of genes). (D) MR1tet− cells comparing individuals with LTBI versus TB neg. (E) Volcano plot comparing MR1tet− cells with MR1tet+ cells. (F) CCR1, CXCR4, CXCR6, TIGIT, IL-7R, and ABCB1 expression at the mRNA (upper panels: gene expression values in counts normalized by sequencing depth calculated by the DEseq2 package) and protein (lower panels: protein expression as percent frequency of subset) levels in memory CD8 T cells and MR1tet− MAITs. Gene expression data were derived from memory CD8 T cells from 17 individuals and MR1tet− cells (n individuals = 12) using an Illumina sequencing platform. Protein expression data were derived from memory CD8 T cells from 20 individuals and MR1tet− cells (n individuals = 7) using flow cytometry. Median ± interquartile range is shown. Two-tailed Mann–Whitney U test.
