Abstract
The specificity of rabbit antibodies to neutral salt-soluble rat skin collagen was studied by the aid of cyanogen bromide peptides. Early antisera contained mainly antibodies directed to C-terminal sequences of the α1 as well as of the α2 polypeptide chains. During hyperimmunization these titers usually were reduced and new α2-chain specific antibodies appeared. Inhibition tests demonstrated that they are directed to the N-terminal sequence. An increase in antigen dosage did not alter the early response. No exclusive relation of one of the different specificities to IgM antibodies could be demonstrated. The results were discussed in terms of competition between distinct antigenic determinants in the collagen molecule.
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Copyright © 1972 by The American Association of Immunologists, Inc.
1972
The Williams & Wilkins Company
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