A sensitive assay system using syngeneic attacker and target cells was developed to characterize the cellular and serum components active in antibody-dependent cellular cytotoxicity (ADCC). Lysis of 51Cr-labeled EL-4 target cells requires only 4-hr incubation in vitro in the presence of normal mouse cells and heat-inactivated anti-EL-4 serum. Cytotoxicity was obtained with high serum dilutions of either xenogeneic (rabbit) or allogeneic (BALB/c) antiserum. The IgG fraction obtained from the rabbit anti-EL-4 serum was also effective in this system. Adherent cells from spleen and peritoneal exudate as well as non-adherent cells from the spleen of normal C57BL/6 mice were effectively cytotoxic in conjunction with the appropriate antibody. These observations indicate that at least two populations of cells may act as effector cells in the ADCC phenomenon.

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Copyright © 1973 by The American Association of Immunologists, Inc.
1973
The Williams & Wilkins Company
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