Summary
The hemagglutinating properties of serum and plasma that develop on inoculation of Corynebacterium H or filtrates of its cultures are not related to changes in the pH of serum or plasma. The fraction of the serum or plasma essential for the formation of bacteriogenic hemagglutinins is destroyed by heating at 65 C for ten minutes.
Addition of yeast or of fractions of vitamin B to serum or plasma inoculated with Corynebacterium H favors the development of bacteriogenic hemagglutinins. No such effect was observed when growth-activating substances were added to the serum simultaneously with filtrates of the bacterium. A medium which yields filtrates that are not agglutinogenic when added to serum or plasma, produces filtrates with agglutinogenic properties when yeast-extract is added to the growing culture.
Bacteria which fail to produce changes in the agglutinating properties of serum do not behave differently when yeast is added to the serum in which they are growing.
The agglutinogenic fraction of the serum is partly or completely exhausted in the course of the formation of bacteriogenic agglutinins. After adsorption of the agglutinins only minute traces of the agglutinogenic fraction of the serum are left, which can be transformed into hemagglutinins by reinoculation of the adsorbed serum with the bacterium, but only when yeast-extract is added. If the bacteriogenic agglutinin deteriorates on standing reinoculation with the bacterium or with its filtrates fails to produce a second crop of hemagglutinins.
The serums which acquire new hemagglutinating properties do not change in their behavior in relation to certain bacteria.
Further evidence is added to show the independence of the normal t-agglutinin and of the bacteriogenic hemagglutinin (h-agglutinin).
The ability to react with bacteriogenic h-agglutinin is not limited to red cells, but is found also in various tissues.
Certain bacteriostatic and bactericidal agents are capable of preventing the development of bacteriogenic hemagglutinins in serum or plasma.