The solubility of equine tetanal antitoxin in methanol-water mixtures of controlled pH, ionic strength, and temperature has been determined.

On the basis of these determinations, three steps have been developed for the separation of tetanal antitoxin from other protein components of plasma. The relatively high ionic strength (0.2) at pH 5 and 10 per cent methanol which is employed in the second step contributes most to this separation.

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