A method for concentrating DPNase from streptococcal cultures has been described. A high degree of purification of the enzyme was achieved by absorption on alumina gel, elution at alkaline pH followed by salt fractionation and finally by continuous flow curtain electrophoresis. Immunochemical studies of DPNase-anti-DPNase system were carried out using the purified enzyme and a globulin fraction prepared from rabbit antiserum.

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