It has been demonstrated that at low ionic strength nucleoprotein removes greater quantities of antinuclear factors from serum. These factors can be dissociated from nucleoprotein and obtained in fairly pure form by increasing the ionic strength of the system.

A study of these preparations has showed that they contain principally 6.6 S γ-globulin and lesser amounts of β2-macroglobulin and β2A-globulin. Antinuclear factor (ANF) activity has been demonstrated by positive indirect fluorescent antibody tests.

In a random sample of 10 normal sera, 6.6 S γ-globulin-ANF has been detected in levels ranging from 0.51 to 0.73 mg/ml.

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