Since Landsteiner and Chase first demonstrated passive transfer of sensitivity to picryl chloride by peritoneal exudate cells in guinea pigs (1), numerous studies have established the general nature of this phenomenon (2–5). In most cases biologic antigens, e.g., toxoids, have been employed by a variety of routes, and either circulating antibodies (2–4, 6, 7), delayed hypersensitivity (1) or both (8) have been detected.

The purpose of this study was to determine the extent of induced protection against lethal doses of tetanus toxin following passive transfer of sensitized lymph node cells (SLNC) in nonirradiated, adult, highly inbred mice. The mouse has been shown to be susceptible to tetanus toxin (9), and active immunization with tetanus toxoid has been produced in this animal (10).

Materials and Methods. The animals used were C3H/HeJ3 adult female mice. Fifty animals were immunized by injecting 0.25 ml of aluminum phosphate-adsorbed tetanus toxoid4 into the base of the tail and an equal amount subcutaneously into the interscapular region.

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