Three volunteers were experimentally infected with adenoviral types 1 or 4, and six were inoculated with purified preparations of type 1 or type 4 soluble hexon and fiber antigens. Neutralizing antibodies and immunoglobulins were assayed after fractionation of serum by gel filtration, revealing generally similar patterns of antibody formation in men given infectious virus or soluble antigens.

Antibody activity was first detected in the immunoglobulin pools one to three weeks after inoculation. In 7 of the 9 men, neutralizing antibodies were present in both the IgM and IgG pools at this time. No serum samples were assayed earlier than 1 week after inoculation. IgM antibodies were found no later than 4 weeks after administration of soluble antigens, but were found up to 14 weeks after inoculation in two of the experimentally infected men. IgG antibodies persisted as long as the volunteers were followed, up to 50 weeks in one man. This persistence was also true for the heterologous responses studied. Antibody titers were low in the experimentally infected volunteers, and neutralizing activity appeared in their immunoglobulin pools a week later than in pools from subjects given soluble antigen preparations of the corresponding serotype.

Serum neutralizing antibodies of the IgA-globulin class were demonstrated in two volunteers, 2 and 3 weeks after inoculation of type 4 adenoviral soluble antigens. None could be detected in a volunteer who had been infected experimentally.

This content is only available via PDF.
You do not currently have access to this content.