The reaction of specific antigens with leucocytes from sensitive donors has been studied. Under appropriate conditions antigen will combine with the cells at 37°C or at 4°C and in the presence or absence of Ca++ and Mg++ without the release of significant quantities of intracellular histamine. Washed primed cells will release their histamine upon incubation at 37°C in buffer containing Ca++ and Mg++ but in the absence of added antigen.

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