Garg, A., P. F. Barnes, A. Porgador, S. Roy, S. Wu, J. S. Nanda, D. E. Griffith, W. M. Girard, N. Rawal, S. Shetty, and R. Vankayalapati. 2006. Vimentin expressed on Mycobacterium tuberculosis-infected human monocytes is involved in binding to the NKp46 receptor. J. Immunol. 177: 6192–6198 .
Subsequent to publication of the above manuscript, an investigation by The University of Texas Health Science Center at Tyler found that Dr. S. Roy committed scientific misconduct pertaining to this publication. Because Dr. Roy produced the data for Fig. 3 in the paper cited above and was unable to provide the original data, the experiments were repeated and the results are shown in the new figure below. As a result of the new figure, the following changes need to be made to the published article.
In Materials and Methods, in the paragraph titled Fluorescence microscopy, the last two sentences “Slides were examined with a Zeiss LSM 510 confocal laser scanning microscope using a C-Apochromat ×63, 1.2 water immersion objective (Carl Zeiss), and the 488-nm line of the argon laser for excitation of Oregon Green. The cells were scanned and images saved at 1024 × 1024-pixel/8-bit resolution before importing into Adobe Photoshop (Adobe Systems) for compilation and direct printing.” should be replaced with the sentence “Slides were examined with a fluorescence microscope (Olympus BX50).”
In Results, in the paragraph titled Surface expression of vimentin on monocytes infected with M. tuberculosis H37Ra and Listeria, the text “Infected monocytes were stained with murine anti-vimentin primary Ab and Oregon Green 488-conjugated goat anti-mouse IgG Ab secondary Ab, and the stained cells was analyzed by fluorescence microscopy.” should read “Infected monocytes were stained with FITC anti-vimentin Ab and the stained cells were analyzed by fluorescence microscopy.” The sentence “Cells incubated with blocking buffer alone, or with isotype control mouse Abs, were used as controls” should be deleted.