Abstract
In murine malaria models, liver resident memory CD8+ T cells are important for protective immunity as they efficiently eliminate malaria infected hepatocytes in vivo. Further characterization of liver resident memory CD8+ T cells is therefore very crucial for the design of an effective anti-malaria vaccine. Here, we describe a microarray approach aims to elucidate the genes and pathways that may involve in the homing, function and differentiation of liver resident memory T cells. We compared the gene expression profiles of antigen specific CD8+ T cells sorted from liver and those from spleen of mice 30-45 days after immunization with irradiated Plasmodium yoelii sporozoites. Preliminary analysis suggests memory CD8+ T cells from liver and spleen have very distinct gene expression profiles. We found clusters of genes enriched in biological functions such as adhesion, metabolic process, cell cycle and signal transduction. The results suggest that CD8+ T cells reside in peripheral organs such as the liver, may have undergone a different program of development than those memory cells that reside in secondary lymphoid organs such as the spleen.
