Correction: Dendritic Cell RIPK1 Maintains Immune Homeostasis by Preventing Inflammation and Autoimmunity

DC necroptosis underlies the systemic inflammation in Ripk1^{DC KO} mice. (A) DCs from CD11cCre Ripk1^fl/D138N (Ripk1^{DC KO/D138N}) and Ripk1^{DC KO} Ripk3^−/− mice are protected from necroptosis in vitro. BMDCs were treated with SMAC mimetics and zVAD-fmk. Effects on cell viability were measured at 18 h using Cell Titer Glo. n = 3–6 samples. (B) Spleen and lymph node (LN) weights and lymph node cellularity from CD11cCre, Ripk1^{DC KO}, Ripk1^{DC KO/D138N}, Ripk1^{DC KO} Ripk3^−/−, and Ripk1^{DC KO} Mlkl^−/− mice. (C) Neutrophil and inflammatory monocyte numbers in the spleens of CD11cCre, Ripk1^{DC KO}, Ripk1^{DC KO/D138N}, Ripk1^{DC KO} Ripk3^−/−, and Ripk1^{DC KO} Mlkl^−/− mice. (D) H&E and Masson’s trichrome staining of spleens from Ripk1^{DC KO}, Ripk1^{DC KO/D138N}, Ripk1^{DC KO} Ripk3^−/−, and Ripk1^{DC KO} Mlkl^−/− mice. (E) Neutrophils, inflammatory monocytes, and B cells are elevated in the lymph nodes of Ripk1^{DC KO} and Ripk1^{DC KO} Ripk3^−/− mice but not Ripk1^{DC KO/D138N} or Ripk1^{DC KO} Mlkl^−/− mice. (F) Proportion of mice serum positive for ANAs at 6 mo (n = 3–6 mice per genotype). Unless otherwise stated, all data shown are from 16 wk-old mice. CD11cCre and Ripk1^{DC KO} mouse phenotyping data shown in (B), (C), (E), and (F) were shown in Fig. 2. Error bars, mean ± SEM. Scale bars, 100 μm. Unpaired two-tailed Student t test (A–C and E). *p < 0.05, **p < 0.01.