Three preliminary experiments comprising a group of 18 animals indicated that a significant immune response might be obtained by the subcutaneous inoculation of the equivalent of 1 to 2 grams of purified virus adsorbed on aluminum hydroxide gel.
Of a group of 10 animals receiving the equivalent of 1.5 grams of virus inoculated in three doses of 5 cc. each subcutaneously, at weekly intervals, the serums of 7 neutralized the test dose of virus and 7 of the animals have thus far resisted increasing doses up to 1.6 cc. of a 5 per cent suspension of a very potent virus (VM no. 8). Of the 10 animals inoculated with the equivalent of 2 grams of virus, the serums of 8 neutralized the test dose of virus. Of the 8 animals who lived sufficiently long to permit the intracerebral test, 5 neutralized the same test dose intracerebrally and three of these animals have thus far resisted increasing doses up to 1.6 cc. of a 5 per cent suspension of potent virus (VM no. 8).
This work is supported by grants from the Rockefeller Foundation, the Friedsam Foundation, and by a grant from the President's Birthday Ball Commission for Infantile Paralysis Research.