Conditions which led to inactivation of rabbit γG-globulin in direct passive cutaneous anaphylaxis (PCA) experiments in guinea pig skin also produced inactivation in reverse PCA experiments. Thus acetylation, carbamylation, picrylation and phthylation of reverse PCA antigens (rabbit Fc or dinitrophenylated rabbit γ-globulin) caused loss of skin-sensitizing activity whereas amidination did not. These results strongly favor the possibility that inactivation of skin-sensitizing activity is due to loss of fixation in guinea pig skin. In further support of this view no correlation was seen between complement-fixing activity and skin-sensitizing activity. Amidinated preparations of rabbit antibody which retained their ability to sensitize guinea pig skin fixed very little complement.

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Presented in part at the 51st Annual Meeting of the Federation of American Societies for Experimental Biology, April 1967 (Fed. Proc. 26: 309 (Abstr. 301).) This work was supported by United States Public Health Service Grants AI00219, AI04646 and FO5TW990.

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Copyright © 1968 by The American Association of Immunologists, Inc.
1968
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