Abstract
Antibody to human IgG was measured by its ability to neutralize herpes simplex virus (HSV) that had been sensitized with human anti-HSV IgG. Incubation of IgG-sensitized virus with anti-IgG showed that a linear relationship existed between the amount of virus neutralized and the concentration of anti-IgG. The reciprocal of the highest dilution of the anti-IgG serum that neutralized at least 50% of the IgG-sensitized virus was taken as the titer of the serum. The titer of the anti-IgG was affected by the concentration of anti-HSV IgG used to sensitize the virus, the concentration of sensitized virus and the amount of non-viral-bound human IgG in the fluid phase of the reaction mixture. The measurement of anti-IgG by the neutralization of sensitized virus was shown to be as sensitive and possibly more sensitive than the measurement of anti-IgG by the hemagglutination technique.
