Abstract
Canine intestinal secretions from the jejunum, terminal ileum, and proximal colon contain qualitatively the same immunoglobulins as do the serum and colostrum. Secretory IgA is present in the fecal extracts in four to eight times the concentration found in serum. A procedure for minimizing the bacterial and enzymatic proteolysis of the immuno-proteins is outlined. A combination of DEAE and Sephadex chromatography permitted separation of the major immunoglobulin classes, particularly secretory IgA. The purified secretory IgA was examined for antigenic similarities to IgA in serum and other canine secretions, and its sedimentation and electrophoretic behavior were defined. The isolated IgA retained biologic activity.
Footnotes
Precedes III, Distribution of Immunoglobulins in Colostrum and Isolation of Secretory IgA and 7Sγ1, which appears on page 1000.
