Abstract
Following three courses of immunization with type VIII pneumococci, the serum of one rabbit showed an unusually marked increase in electrophoretically-restricted γ globulin components consisting of anti-polysaccharide antibodies. Examination of the intact purified antibodies by isoelectric focusing and of the corresponding light chains by disc gel electrophoresis in urea disclosed a less complex banding pattern than was observed with antibody isolated from serum of a rabbit showing a diffuse increase in globulin components after immunization. The serum concentration of the electrophoretically-restricted γ globulin components increased and subsequently decreased after each course of immunization. Histologic examination following the third course showed evidence of an intense immune response; plasma cell tumors were not seen.
Footnotes
This work was supported by United States Public Health Service Research Grants AI-04967, HE-06664, HE-5196, and Grant T-451 from the American Cancer Societies, Inc. A preliminary report of this work has been presented (Fed. Proc., 28: 695, 1969). Request for reprints should be addressed to: Dr. Edgar Haber, Cardiac Unit, Massachusetts General Hospital, Boston, Massachusetts 02114.
