Abstract
Macrophages obtained from the peritoneal cavity of mice were incubated in vitro with human γ globulin (HGG), and then transferred to allogeneic recipients that were boosted 30 days later with soluble antigen. Recipients made better primary and secondary responses to HGG bound to macrophages than did animals immunized with soluble HGG.
The immunogenicity of HGG in macrophages was moderately decreased by treatment of the cells with concentrations of actinomycin-D that inhibited RNA synthesis.
These results are discussed in the light of the possible importance of informational RNA and of increased immunogenicity of macrophage-bound antigen in the immunologic response.
Footnotes
This work was supported by Grants 5001, 5014 and 5029 from the Swiss National Foundation for Scientific Research.
