The syngeneic mixed lymphocyte reaction between thymus cells and peripheral lymphoid cells was investigated in CBA, C57BL and BALB/c mouse strains. Four-day-old thymus cells from CBA and BALB/c mice proliferate in culture with syngeneic Mitomycin C-treated lymphoid cells. The reaction is smaller (or not significant) when the thymus cells are prepared from older mice. In contrast, 4-day-old C57 thymus cells do not respond significantly to syngeneic lymphoid cells, although they respond well to allogeneic cells. CBA and BALB/c peripheral lymphoid cells may possess an antigen “other than H2” responsible for the stimulation of syngeneic thymus cells.

The target cell for the syngeneic mixed lymphocyte reaction in CBA mice is a lymphocyte present in spleen, peripheral blood, lymph nodes and peritoneal exudate.


This work was supported by grants from the Australian Research Grants Committee and the National Health and Medical Research Council, Canberra, Australia. This is publication 1733 from the Walter and Eliza Hall Institute.

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