Cell Interactions Between Histoincompatible T and B Lymphocytes: VIII. In Vivo Cooperative Responses Between Lymphocytes Are Controlled by Genes in the K-End of the H-2 Complex¹

The genetic restrictions for successful carrier-primed T cell and hapten-primed B cell cooperative interactions were established in a totally in vivo double adoptive transfer system. In complementation experiments, we have demonstrated that genes localized in the K-end of the H-2 complex control successful T-B cell cooperative interactions. Thus, DBA/2 KLH-primed T cells cooperated with (C3H × C3H.OH)F₁ and (C3H × C3H.OL)F₁ DNP-primed B cells to make a secondary response to DNP-KLH, but not with C3H or (C3H × C3H.A)F₁ DNP-primed cells. The presence of foreign histocompatibility determinants on the F₁ B cells did not prevent successful physiologic cell cooperation provided the T and B cells shared common cell interaction genes coded for in the K-end of the H-2 complex.