The footpad swelling (FPS) test for delayed-type hypersensitivity in the mouse was evaluated for its ability to measure histocompatibility (H) antigens present on intact spleen cells or solubilized from a methylcholanthrene-(MCA) induced fibrosarcoma.

Mice were immunized to H-antigens (H-2 or non-H-2) with skin allografts and challenged 14 days later with test antigens. Spleen cell-bound and solubilized H-antigens elicited strong and specific FPS reactions in allograftimmunized but not in unimmunized mice. The test could distinguish H-2 from non-H-2 antigens as well as D from KI region specificities of the H-2 gene complex.

These experiments suggest that the FPS test is a reliable in vivo method for measuring both cell-bound and solubilized H-antigens in the mouse.

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This is Tumor Biology Unit Publication No. 106 and was supported in part by Grants CA-13384, CA-15334, CA-09126, and HD-00384 from the National Institutes of Health, Bethesda, Maryland.

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Copyright © 1976 by The American Association of Immunologists, Inc.
1976
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