Immunogen Structure and Cell Selection: I. Cellular Requirements for the in Vitro Response to Moderately Haptenated Forms of the Solid-Phase Immunogen DNP-O-Bio-Gel-P¹

The cellular requirements for the in vitro response to DNP-O-Bio-Gel-P were determined. Results of these experiments indicate that: 1) splenic adherent cells are required for generation of anti-hapten PFC to the solid phase immunogen; and 2) cultured nude-mouse spleen cells have far less capacity to respond to the immunogen than do cells from wild type mice. These experiments suggest that both T and B cells are required for responsiveness to the moderately haptenated form of DNP-O-Bio-Gel-P.