Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3 mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.


This work was supported by grants from the United States Public Health Service, National Institutes of Health AI 12478, the Kroc Foundation, and an American Cancer Society Institutional Research Grant IN-13. A preliminary report of these findings was presented at a poster session at the Seventeenth Annual Meeting of the American Society of Cell Biology (Abstract, J. Cell Biol. 75: 210a, 1977).

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