Anti-idiotypic sera specific to anti-(T,G)-A—L antibodies of high responder C3H.SW (H-2b, Ig-1a) mice were prepared in guinea pigs. The anti-idiotypic sera bind specifically 30% of the iodinated antibodies of C3H.SW against (T,G)-A—L and inhibit antigen binding of these antibodies. These anti-idiotypic sera do not react with anti-(T,G)-A—L antibodies produced in the congenic strain, CWB (H-2b, Ig-1b), which differ from the C3H.SW strain by allotype, but cross-react with anti-(T,G)-A—L produced in BALB.B10 (H-2b, Ig-1a), which differ from C3H.SW in its genetic background but possess the same allotype. These results suggest a linkage between the idiotypic determinants analyzed and Ig-1a allotype.
Immunization of C3H.SW mice with the ordered peptide antigen (T-T-G-G-)-A—L led to the production of antibodies that share idiotypic determinants with C3H.SW anti-(T,G)-A—L antibodies, confirming previous conclusions that Tyr-Tyr-Glu-Glu is the major determinant of the random (T,G)-A—L. Anti-(T,G)-A—L antibodies produced in low responders C3H/DiSn (H-2k, Ig-1a) by immunization with (T,G)-A—L complexed with methylated bovine serum albumin (MBSA) cross-react with the anti-idiotypes prepared against the high responder anti-(T,G)-A—L antibodies. The low responder antibodies were approximately 3 to 5-fold less efficient in inhibiting the idiotypes-anti-idiotypes reaction. However, anti-(T,G)-A—L produced in high responders by immunization with (T,G)-A—L + MBSA were identical to the low responder antibodies in their idiotypic content as judged by inhibition of the binding of the idiotypes to the anti-idiotypes.
These results demonstrate that high and low responders that possess Ig-1a allotype have a similar repertoire of B cells producing anti-(T,G)-A—L antibodies.
This work was supported by Grants 1RO1 AI 11405-05 and 1RO1 AI CA 13200-02 from the National Institutes of Health, United States Public Health Service, Bethesda, Maryland.