Extensive genetic, cellular and molecular studies have suggested that I region cell surface gene products of the MHC play an important role in cell-cell recognition during T-dependent humoral immune responses. Our own studies have demonstrated that sharing of genes in the Ir-1A or Ir-1B subregions of the I region (as defined by the B10.A(5R) and B10.A(4R) combination) between collaborating T and B lymphocytes is essential for primary B-cell stimulation to IgG1 antibody synthesis in response to DNP-Hy. We have utilized the I region genetically controlled responses to the polymer GLϕ to better define the precise nature of recognition restrictions in T cell-B cell interactions in antibody responses to DNP-GLϕ in the splenic fragment culture system. In this system syngeneic and allogeneic T cell-B cell collaborations can be discriminated by virtue of the Ig heavy chain isotope of the antibody which is synthesized as a result of the cell-cell interaction.

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