I-region-coded nonresponder T cell-derived suppressor extracts (TsF) specific for l-glutamic acid60-l-alanine30-l-tyrosine10 (GAT) or l-glutamic acid50-l-tyrosine50 (GT) induce T suppressor cells (Ts2) in vivo and in vitro in nonresponder spleen cells. This induction requires both an I-J subregion coded TsF and also specific antigen. The Ts2 cells are I-J+ and do not acquire these I-J determinants from passively adsorbed TsF. Further, Ts2 are highly antigen specific, being able to selectively suppress GAT vs GT-MBSA responses in the same strain. In the GT model, H-2a mice fail to produce GT-TsF, while H-2b mice cannot yield active Ts2 when exposed to GT-TsF. (B6A)F1 mice complement for GT suppression by reciprocal parental contribution of the missing suppressor components. No H-2 identity requirements for Ts2 generation by TsF have been noted in nonresponders, and Ts2 induction by nonresponder TsF can also occur in responder spleen cells, indicating lack of requirement for Ir matching between TsF donor and recipient.