An antiserum was produced in C3H/He mice that can recognize the antigenic variations of murine C3. The alloantiserum is directed to the gene product of one of the alleles (C3-1a allele) of the C3-1 locus that controls genetic variations of murine C3 and is linked to H-2. The antiserum formed a single precipitin line in the Ouchterlony test with sera obtained from mice that carry the C3-1a/a or C3-1a/b genotype, but it did not react with sera from mice that do not have C3 coded for by the C3-1a allele. By the use of the alloantiserum, we detected a new C3 allotype (C3-1 CC) in SWR/J mice. The C3-1 CC is distinct from the other two allotypes on the basis of antigenicity and isoelectric point (pI). Therefore, at least three alleles have been identified for the C3-1 locus.

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This work was supported in part by a Grant-in-Aid for special projects (Nos. 11504 and 210704) from the Ministry of Education, Science and Culture, the Government of Japan.

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