Quantitative Studies on the Action of Compound Hemolysins: I. Graphical Representation of the Reaction Between Amboceptor, Complement and Blood Corpuscles

A method of plotting hemolytic data was developed and it was shown that curves representing the same percentage of hemolysis are parallel, as long as the concentration of blood cells in the mixture is not changed, and can be made to coincide by a shift of one or both coördinate axes. The shape of the curves is, therefore, independent of the concentration or titer of amboceptor and complement serum. This conclusion was derived under certain assumptions, the principal one being that the components are individual substances and not mixtures of two or three. A comparison of the shape of such “characteristics” should, therefore, permit conclusions as to the purity of the components used for the tests.