Andreas Hombach, Anja Wieczarkowiecz, Thomas Marquardt, Claudia Heuser, Loretta Usai, Christoph Pohl, Barbara Seliger, and Hinrich Abken. Tumor-Specific T Cell Activation by Recombinant Immunoreceptors: CD3ζ Signaling and CD28 Costimulation Are Simultaneously Required for Efficient IL-2 Secretion and Can Be Integrated into One Combined CD28/CD3ζ Signaling Receptor Molecule. The Journal of Immunology 2001;167:6123–6131 .
In Materials and Methods, the oligonucleotides number 1 and 2 given in Table I contain incorrect sequences. The correct table is shown below.
Table I.
Oligonucleotide primers used for the generation of recombinant immunoreceptors
| 1-CD28 BamHI-sense: 5′-CTGGATCCCAAATTTTGGGTGCTGGTGGTGGTTG-3′a |
| 2-CD28-XhoI-antisense: 5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′ |
| 3-CD28-CD3ζ-antisense: 5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′b |
| 4-CD28-CD3ζ-sense: 5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGGAGC-3′b |
| 5-CDζ-XhoI-antisense: 5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′ |
| 6-Lκ-NcoI-sense: 5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′ |
| 7-hIgG1Fc-BglII-antisense: 5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′ |
| 1-CD28 BamHI-sense: 5′-CTGGATCCCAAATTTTGGGTGCTGGTGGTGGTTG-3′a |
| 2-CD28-XhoI-antisense: 5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′ |
| 3-CD28-CD3ζ-antisense: 5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′b |
| 4-CD28-CD3ζ-sense: 5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGGAGC-3′b |
| 5-CDζ-XhoI-antisense: 5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′ |
| 6-Lκ-NcoI-sense: 5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′ |
| 7-hIgG1Fc-BglII-antisense: 5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′ |
a
Restriction sites are underlined.
b
Overlapping sequences are in boldface.
Copyright © 2004 by The American Association of Immunologists
2004
