Andreas Hombach, Anja Wieczarkowiecz, Thomas Marquardt, Claudia Heuser, Loretta Usai, Christoph Pohl, Barbara Seliger, and Hinrich Abken. Tumor-Specific T Cell Activation by Recombinant Immunoreceptors: CD3ζ Signaling and CD28 Costimulation Are Simultaneously Required for Efficient IL-2 Secretion and Can Be Integrated into One Combined CD28/CD3ζ Signaling Receptor Molecule. The Journal of Immunology 2001;167:6123–6131 .

In Materials and Methods, the oligonucleotides number 1 and 2 given in Table I contain incorrect sequences. The correct table is shown below.

Table I.

Oligonucleotide primers used for the generation of recombinant immunoreceptors

1-CD28 BamHI-sense: 5′-CTGGATCCCAAATTTTGGGTGCTGGTGGTGGTTG-3′a 
2-CD28-XhoI-antisense: 5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′ 
3-CD28-CD3ζ-antisense: 5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′b 
4-CD28-CD3ζ-sense: 5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGGAGC-3′b 
5-CDζ-XhoI-antisense: 5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′ 
6-Lκ-NcoI-sense: 5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′ 
7-hIgG1Fc-BglII-antisense: 5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′ 
1-CD28 BamHI-sense: 5′-CTGGATCCCAAATTTTGGGTGCTGGTGGTGGTTG-3′a 
2-CD28-XhoI-antisense: 5′-CTACTCGAGGATCAGGAGCGATAGGCTGCGAA-3′ 
3-CD28-CD3ζ-antisense: 5′-GTCTGCGCTCCTGCTGAACTTCACTCTCAGGGAGCGATAGGCTGCGAAGTC-3′b 
4-CD28-CD3ζ-sense: 5′-CCTATCGCTCCCTGAGAGTGAAGTTCAGCAGGAGC-3′b 
5-CDζ-XhoI-antisense: 5′-CTACTCGAGGATTAGCGAGGGGGCAGGGC-3′ 
6-Lκ-NcoI-sense: 5′-CTACGTACCATGGATTTTCAGGTGCAGATTTTC-3′ 
7-hIgG1Fc-BglII-antisense: 5′-CCCACCCAGATCTTTTTTACCCAGAGACAGGGAGAG-GCTCTTCTG-3′ 
a

Restriction sites are underlined.

b

Overlapping sequences are in boldface.