Shedding of the Type II IL-1 Decoy Receptor Requires a Multifunctional Aminopeptidase, Aminopeptidase Regulator of TNF Receptor Type 1 Shedding.

Xinle Cui, Farshid N. Rouhani, Feras Hawari, and Stewart J. Levine. Shedding of the Type II IL-1 Decoy Receptor Requires a Multifunctional Aminopeptidase, Aminopeptidase Regulator of TNF Receptor Type 1 Shedding. The Journal of Immunology 2003;171:6814–6819

In Results, the authors wish to provide clarification of the experiments using the arts-1^(−/−) cell lines in Figures 3 and 4.

Although the cloned arts-1^(−/−) cell lines utilized in this study did not express either ARTS-1 protein or mRNA, and arts-1 exons 5 and 6 could not be amplified by PCR of genomic DNA, subsequent experiments have revealed detectable ARTS-1 protein expression in these cell lines. Therefore, the arts-1^(−/−) cell lines are not clonal populations of cells homozygous for the targeted deletion of arts-1 exons 5 and 6. This was confirmed by PCR of genomic DNA using the arts-1 exon 5/6 primer pair. As shown below in Figure 8, a 1328 PCR product was generated from the wild-type NCI-H292 cells, as well as from the three arts-1^(−/−) cell lines (#1–#3), which demonstrates the presence of arts-1 exons 5 and 6. Since the arts-1^(−/−) cells that were used for the experiments presented in this paper expressed neither ARTS-1 protein nor mRNA at the time the experiments were performed, we believe that the conclusions regarding the role of ARTS-1 in IL-1R shedding are valid.