Munthe, L. A., A. Os, M. Zangani, and B. Bogen. 2004. MHC-restricted Ig V region-driven T-B lymphocyte collaboration: B cell Receptor ligation facilitates switch to IgG production. J. Immunol. 172: 7476–7484.

In Table I, in Expt. Bc 4, the B cell donor should be NIP-KLH Id (i.e., Id negative), not NIP-KLH Id+. The corrected table is shown below.

Table I.

Nonlinked Id-driven T-B collaboration, hapten and transfer experiments

Expt.GroupB Cell DonorT CellsRecipient MiceBoostId+ Ig Anti-NIP (μg/ml)aIgG1 Anti-NIP (μg/ml)
Ab NIP-KLH Id+ Id-spec Th2 RAG2−/− NIP-BSA 11 (2) 2.5 (0.5) 
 NIP-KLH Id+ Id-spec Th2 RAG2−/− None 3.0 (2) 0.08 (0.05) 
 Id+ Id-spec Th2 RAG2−/− NIP-BSA 0.9 (0.5) 0.04 (0.01) 
Bc NIP-KLH Id+ Id-spec Th2 C.B-17 NIP-BSA 20 (5) 19 (16) 
 NIP-KLH Id+ Id-spec Th2 C.B-17 None 12 (2) 1.3 (1) 
 Id+ Id-spec Th2 C.B-17 NIP-BSA <0.06 0.4 (0.2) 
 NIP-KLH Id Id-spec Th2 C.B-17 NIP-BSA <0.06 <0.1 
Cd NIP-KLH Id+ Id-spec Th2 RAG2−/− NIP-OVA 18 (3) 1.5 (0.5) 
 NIP-KLH Id+ DO.11.10 Th2 RAG2−/− NIP-OVA 23 (3) 6.3 (1) 
 NIP-KLH Id+ DO.11.10 Th2 RAG2−/− None 2.0 (0.7) <0.05 
Expt.GroupB Cell DonorT CellsRecipient MiceBoostId+ Ig Anti-NIP (μg/ml)aIgG1 Anti-NIP (μg/ml)
Ab NIP-KLH Id+ Id-spec Th2 RAG2−/− NIP-BSA 11 (2) 2.5 (0.5) 
 NIP-KLH Id+ Id-spec Th2 RAG2−/− None 3.0 (2) 0.08 (0.05) 
 Id+ Id-spec Th2 RAG2−/− NIP-BSA 0.9 (0.5) 0.04 (0.01) 
Bc NIP-KLH Id+ Id-spec Th2 C.B-17 NIP-BSA 20 (5) 19 (16) 
 NIP-KLH Id+ Id-spec Th2 C.B-17 None 12 (2) 1.3 (1) 
 Id+ Id-spec Th2 C.B-17 NIP-BSA <0.06 0.4 (0.2) 
 NIP-KLH Id Id-spec Th2 C.B-17 NIP-BSA <0.06 <0.1 
Cd NIP-KLH Id+ Id-spec Th2 RAG2−/− NIP-OVA 18 (3) 1.5 (0.5) 
 NIP-KLH Id+ DO.11.10 Th2 RAG2−/− NIP-OVA 23 (3) 6.3 (1) 
 NIP-KLH Id+ DO.11.10 Th2 RAG2−/− None 2.0 (0.7) <0.05 
a

Total anti-NIP Ab with Vλ1/2 L chain, irrespective of H chain isotype or allotype.

b

Expt. A: Splenocytes from immunized ((NIP)6-KLH)) or nonimmunized Id+ mice were depleted of T cells and injected (107) into RAG2−/− BALB/c mice on day 0 (five mice per group). Id-specific (spec) Th2 cells (6 × 106) and boost (150 μg (NIP)5-BSA in PBS i.p.) were given on day 1 as indicated. Mean (SD) of anti-NIP Ab in day 15 sera is given, p values were calculated by Mann-Whitney Test and are given in main text.

c

Expt. B: Splenic B cells from immunized or nonimmunized Id+ or Id mice were injected (3 × 106) into IgHb C.B-17 mice (n = 5), followed by Th2 cells (5 × 106) and boost as indicated. Ab H chains from transferred B cells were detected by IgG1a-specific ELISA. Data are from day 6, before immune responses in the unirradiated immunocompetent C.B-17 hosts could contribute to an increased background of λ2+ anti-NIP.

d

Expt. C: As Exp. A, but with Id-specific Th2 or DO.11.10 Th2 and (NIP)9-OVA boost.