Swiecki, M. K., M. W. Lisanby, F. Shu, C. L. Turnbough, Jr., and J. F. Kearney. 2006. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J. Immunol. 176: 6076–6084 .
In Table II, the data reported for GA2–3b in column six (ΔrmlD spores) should be negative (−) not 2-log shift (++). The corrected table is shown below.
Table II.
mABs raised against irradiated B. anthracis spores or purified B. anthracis exosporium
| mAbs . | Western . | . | FACS . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Anti-E. coli Bc1A . | Anti-Deglyco, Bc1A . | WT Sterne spores . | Δbc1A spores . | ΔrmlD spores . | |||
| AB2 | NDa | ND | + | − | ++ | |||
| AF10b | + | + | + | − | ++ | |||
| AH88 | + | − | + | − | ++ | |||
| BD8b | + | + | + | ND | ND | |||
| BE12b | + | + | + | − | ++ | |||
| BF1-4b | + | + | + | − | ++ | |||
| BF12 | ND | ND | + | − | ++ | |||
| BG11b | + | − | + | ND | ND | |||
| CA3* | + | − | + | − | ++ | |||
| DE3-1b | + | + | + | − | ++ | |||
| DE12 | − | − | + | − | ++ | |||
| FD3-4b | + | + | + | ND | ND | |||
| EF12b | + | + | + | ND | ND | |||
| AA2-1 | ND | ND | + | − | ++ | |||
| BA10-1b | + | + | + | − | ++ | |||
| DH4-1b | − | − | + | ++ | ++ | |||
| EA2-1 | ND | ND | + | − | ++ | |||
| EA4-10b | + | + | + | − | ++ | |||
| EA4-10-4 | + | + | + | − | ++ | |||
| EG4-4b | + | + | + | − | ++ | |||
| FH6-1b | − | − | + | ++ | ++ | |||
| GA2-3b | − | − | + | − | − | |||
| GB4-4b | − | − | + | ND | ND | |||
| GB4-6-2 | ND | ND | + | − | ++ | |||
| HB2-2 | ND | ND | + | − | ++ | |||
| IC801b | − | − | + | − | ++ | |||
| JB5-1b | + | + | + | − | ++ | |||
| JC8-5sb | + | + | + | − | ++ | |||
| mAbs . | Western . | . | FACS . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| . | Anti-E. coli Bc1A . | Anti-Deglyco, Bc1A . | WT Sterne spores . | Δbc1A spores . | ΔrmlD spores . | |||
| AB2 | NDa | ND | + | − | ++ | |||
| AF10b | + | + | + | − | ++ | |||
| AH88 | + | − | + | − | ++ | |||
| BD8b | + | + | + | ND | ND | |||
| BE12b | + | + | + | − | ++ | |||
| BF1-4b | + | + | + | − | ++ | |||
| BF12 | ND | ND | + | − | ++ | |||
| BG11b | + | − | + | ND | ND | |||
| CA3* | + | − | + | − | ++ | |||
| DE3-1b | + | + | + | − | ++ | |||
| DE12 | − | − | + | − | ++ | |||
| FD3-4b | + | + | + | ND | ND | |||
| EF12b | + | + | + | ND | ND | |||
| AA2-1 | ND | ND | + | − | ++ | |||
| BA10-1b | + | + | + | − | ++ | |||
| DH4-1b | − | − | + | ++ | ++ | |||
| EA2-1 | ND | ND | + | − | ++ | |||
| EA4-10b | + | + | + | − | ++ | |||
| EA4-10-4 | + | + | + | − | ++ | |||
| EG4-4b | + | + | + | − | ++ | |||
| FH6-1b | − | − | + | ++ | ++ | |||
| GA2-3b | − | − | + | − | − | |||
| GB4-4b | − | − | + | ND | ND | |||
| GB4-6-2 | ND | ND | + | − | ++ | |||
| HB2-2 | ND | ND | + | − | ++ | |||
| IC801b | − | − | + | − | ++ | |||
| JB5-1b | + | + | + | − | ++ | |||
| JC8-5sb | + | + | + | − | ++ | |||
a
ND, Not determined, −, negative by Western or FACS; +, positive by Western or l-log shift by FACS; ++, 2-log shift by FACS ABS-EF12, mAbs raised against spores; AA2-I-JC8-5, mAbs and raised against exosporium.
b
Included in Ref. 9.
Copyright © 2006 by The American Association of Immunologists
2006
