Apoptosis of infected cells represents a key host defense mechanism against viral infections. The impact of apoptosis on the elimination of hepatitis C virus (HCV)-infected cells is poorly understood. The TRAIL has been implicated in the death of liver cells in hepatitis-infected but not in normal liver cells. To determine the impact of TRAIL on apoptosis of virus-infected host cells, we studied TRAIL-induced apoptosis in a tissue culture model system for HCV infection. We demonstrated that HCV infection sensitizes primary human hepatocytes and Huh7.5 hepatoma cells to TRAIL induced apoptosis in a dose- and time-dependent manner. Mapping studies identified the HCV nonstructural proteins as key mediators of sensitization to TRAIL. Using a panel of inhibitors targeting different apoptosis pathways, we demonstrate that sensitization to TRAIL is caspase-9 dependent and mediated in part via the mitochondrial pathway. Sensitization of hepatocytes to TRAIL-induced apoptosis by HCV infection represents a novel antiviral host defense mechanism that may have important implications for the pathogenesis of HCV infection and may contribute to the elimination of virus-infected hepatocytes.

Hepatitis C virus (HCV)5 infection is a leading cause of liver cirrhosis and hepatocellular carcinoma (1). HCV belongs to the Flaviviridae family and has an enveloped, positive-stranded RNA genome of 9.6 kb length containing one open reading frame translated into a single polyprotein. A highly conserved, untranslated region at the 5′ site serves as an internal ribosomal entry site which directs cap-independent translation. Posttranslational cleavage of the polyprotein yields in structural and nonstructual proteins including core, E1, E2, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B (2, 3).

Histopathological studies demonstrated that enhanced hepatocyte apoptosis is a common feature in the HCV-infected liver (4, 5). The close physical proximity of apoptotic hepatocytes and infiltrating lymphocytes seen in HCV infection suggests that apoptosis is initiated by an interaction between effector cells of the host immune system and hepatoyctes (4). Effector cells of the innate and acquired immune system are able to kill target cells through ligands of the death receptor family. In contrast to TNF-α and CD95/Fas, TRAIL induces apoptosis only in infected or transformed tumor cells (6). In line with these observations, TRAIL does not induce apoptosis in noninfected healthy hepatocytes in vivo (5, 7). For all three death ligands an up-regulation of expression has been described in chronic HCV infection (4, 5, 8). TRAIL binding induces the formation of a death-inducing signaling complex, resulting in the activation of caspase-8. Active caspase-8 can trigger two signaling pathways, the first involving direct activation of caspase-3, the second involving cleavage of Bid, followed by mitochondria-dependent activation of caspase-9 via cytochrome C release and Apaf-1 activation (9). Hepatocytes most likely represent so-called type-II cells, for which external activation of the death signaling pathway often is insufficient to induce apoptosis and requires in addition amplification by the mitochondrial pathway (intrinsic apoptosis pathway). The latter is affected by oxidative stress, DNA damage, and viral proteins (10, 11). Mitochondria-dependent apoptosis is amplified by proapoptotic Bax, Bad, Bak, and other proteins, while Bcl-2 or Bcl-xL act anti-apoptotic (for review see Ref. 12).

The molecular mechanisms of hepatocyte apoptosis in HCV infection are poorly understood. For both HCV structural and nonstructural proteins, pro- and antiapoptotic properties have been described (13). Viral RNA has been shown to be able to induce apoptosis via activation of protein kinase R and the retinoic acid inducible gene-I-Cardif pathway (14, 15, 16). However, the significance of these interactions for productive viral infection and pathogenesis of HCV-induced liver disease remains unknown. Studies on HCV-host interactions had been hampered for a long time by the lack of an efficient tissue culture model for HCV infection. Thus, alternative model systems, such as recombinant proteins, pseudotype particles, or subgenomic replicons, had been developed for the study of defined aspects of the HCV life cycle (for review see Ref. 17). A major breakthrough for the study of HCV-host cell interaction was the recent establishment of an efficient cell culture system for HCV (18, 19, 20). This model system now allows the study of HCV-host interactions and apoptosis in the context of the complete viral life cycle in human hepatocyte-derived target cells.

To determine the impact of TRAIL on apoptosis of virus-infected host cells, we studied mechanisms of TRAIL-induced apoptosis in a tissue culture model system for HCV infection.

Primary human hepatocytes (PHH) were isolated and cultured as described (21). Human hepatoma cells Huh7.5 have been described (18, 22).

Plasmids pJFH1, pJFH1/ΔE1E2, pJFH1/GND, pSGR-JFH1, pSGR-JFH1/GND, and pFK-Jc1 have been described (19, 23, 24) and are depicted in Fig. 1.

FIGURE 1.

HCV constructs and viral protein expression. A, Constructs: HCV-pJFH1 resulting in the production of infectious viral particles, pJFH1/ΔE1E2 containing a deletion of the HCV envelope protein coding region preventing production of infectious viral particles, pJFH1/GND containing a mutation in NS5B protein preventing viral replication, and subgenomic replicon pSGR-JFH1 (lacking the coding region for HCV structural proteins and NS2, respectively). B, Protein expression from replication-competent constructs. Seventy-two hours following electroporation with HCV RNA transcribed from constructs depicted in A, cells were lysed and subjected to immunoblot analysis using Abs against HCV core, E2, NS3, and NS5A proteins as described in Materials and Methods.

FIGURE 1.

HCV constructs and viral protein expression. A, Constructs: HCV-pJFH1 resulting in the production of infectious viral particles, pJFH1/ΔE1E2 containing a deletion of the HCV envelope protein coding region preventing production of infectious viral particles, pJFH1/GND containing a mutation in NS5B protein preventing viral replication, and subgenomic replicon pSGR-JFH1 (lacking the coding region for HCV structural proteins and NS2, respectively). B, Protein expression from replication-competent constructs. Seventy-two hours following electroporation with HCV RNA transcribed from constructs depicted in A, cells were lysed and subjected to immunoblot analysis using Abs against HCV core, E2, NS3, and NS5A proteins as described in Materials and Methods.

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Electroporation of RNA derived from plasmids pJFH1, pJFH1/ΔE1E2, pJFH1/GND, pSGR-JFH1, pSGR-JFH1/GND, and pFK-Jc1 was performed as described (19, 23, 24). Culture supernatants from HCV JFH16, HCV Jc1, and HCV JFH1/ΔE1E2 RNA transfected cells were harvested, concentrated and used to infect naive Huh7.5 cells (22) as well as primary human hepatocytes. Culture supernatants used to infect naive Huh7.5 cells had a TCID50 of 104/ml for JFH1 and 105-106/ml for Jc1.

Cell lysis, protein determination, Western blot analysis, and densitometric analysis were performed as recently described (25). Cytosolic and mitochondrial protein fractions were obtained using a ProteoExtract Subcellular Proteome Extraction Kit according to the manufacturer’s protocol (Calbiochem). Relative OD was calculated by correlation of the given protein to β-actin; maximal intensity was arbitrarily set to 100. At least three independent experiments were performed; the mean relative OD and the corresponding SEM is given. Protein loading was controlled by staining the membranes with Coomassie-blue and blotting of β-actin. Immunoblot analysis of HCV core, E2, and NS5A protein was performed using monoclonal mouse anti-core (28), anti-E2 (26), and polyclonal anti-NS5A Abs (27). mAbs directed against NS3 (ViroStat), caspase-3 (3G2, Cell Signaling Technology), caspase-8 (1C12, Cell Signaling Technology), caspase-9 (C9, Cell Signaling Technology), β-actin (Sigma-Aldrich), cleaved poly (ADP-ribose) polymerase (PARP) (Asp214, Cell Signaling Technology), TRAIL-R1, -R2, and TRAIL (R&D Systems), Bcl-2 and Bcl-xL (Santa Cruz Biotechnology), cytochrome C (Biovision), and HRP-linked species-specific Abs (Amersham Biosciences) were used for immunoblot analysis of the respective proteins.

Naive and HCV RNA transfected Huh7.5 cells were seeded at a density of 2–4 × 105 cells/well in 6-well plates 72 h before apoptosis experiments. Cells were washed with PBS and incubated in cell culture medium with recombinant TRAIL that includes the extra cellular domain of human TRAIL (aa 95–281) fused at the N terminus to a His-tag and a linker peptide (SuperKillerTRAIL, Axxora). Alternatively, cells were preincubated in the presence or absence of caspase inhibitors (caspase-3 inhibitor (2-(4-Methyl-8-(morpholin-4-ylsulfonyl)-1,3-dioxo −1,3-dihydro-2H-pyrrolo [3,4-c]quinolin-2-yl)-ethyl-acetate), caspase-8 inhibitor: Z-IETD-FMK, caspase-10 inhibitor: Z-AEVD-FMK, caspase-9 inhibitor: Z-LEHDFMK; all from R&D Systems), Bcl-2/Bcl-xL inhibitors (dimethoxy-dihydro-dibenzodiazocine dioxide and dimethoxy-dinitrosobenzyl; Calbiochem), or Bcl-xL mimetic peptide (Calbiochem) containing the human conserved N-terminal homology domain of Bcl-xL (aa 4–23), linked to a carrier peptide. Subsequently, cells were washed with PBS and harvested in lysis buffer for Western blot analysis or fixed for immunofluorescence staining.

Fixation and immunostaining of Huh7.5 cells was performed using monoclonal anticore Abs (C1/C2) as described recently (25, 28, 29). Cells were embedded in fluorescence-mounting medium containing DAPI (4–6-daimidino-2-phenylindole; Vector Laboratories) for cell nuclei staining. Microscopy was performed using a fluorescent microscope (Leica). For detection of DNA fragmentation, a TUNEL assay was used as previously described (30). The percentage of apoptotic cells was determined by counting the number of TUNEL-positive cells. At least 300 cells were counted out of five microscopic fields at ×400 magnification for each experiment.

Data are expressed as means ± SEM (n = number of cell preparations). SEM was omitted in the figures when smaller than symbol size. Each experiment was performed at least in three independent cell preparations. Results were compared using the Student’s t test; p < 0.05 was considered statistically significant.

Sensitivity of human hepatoma cells to induction of apoptosis has been shown to differ among various cell lines. This is illustrated by the fact that for Huh7 cells, the parental cell line of Huh7.5, apoptosis induction as well as resistance following interaction with TRAIL have been reported (31, 32, 33). Thus, we studied mechanisms of TRAIL-induced apoptosis in Huh7.5 cells, a target cell line for infectious recombinant HCVcc. As shown in Fig. 2, TRAIL rapidly and dose-dependently induced cleavage of caspase-8, -9, and -3 (Fig. 2). The amounts of cleaved and uncleaved caspase-8, caspase-9, and PARP declined during apoptosis. This decline most likely reflects degradation of these proteins due to apoptosis-activated proteases as described in many model systems for apoptosis (34, 35, 36). Caspase activation is a marker of the susceptibility of the cell to undergo apoptosis, but not equivalent to apoptosis (for review, see Ref. 9). To confirm that Huh7.5 cells indeed underwent irreversible apoptosis following TRAIL incubation, we analyzed PARP cleavage, a marker of irreversible cell death (37). After incubating Huh7.5 cells with TRAIL at a concentration of 50 ng/ml for 2 h, cleavage of PARP was detectable (Fig. 2). These data demonstrate that TRAIL induces apoptosis in Huh7.5 hepatoma cells in a dose-dependent manner.

FIGURE 2.

TRAIL-induced apoptosis in human Huh7.5 hepatoma cells is caspase and Bcl-2/Bcl-xL dependent. Following incubation with TRAIL, Huh7.5 cells were lysed and protein lysates were subjected to SDS-PAGE and Western blot analysis using anti-human PARP and caspase-specific Abs. A, left panel, Time course of TRAIL-induced apoptosis with PARP-, caspase-3, caspase-8, and caspase-9 cleavage following 1–24 h incubation with TRAIL at a concentration of 50 ng/ml. Right panel, Dose-dependent induction of TRAIL-dependent apoptosis at TRAIL concentrations ranging from 1 to 200 ng/ml (incubation time 2 h). B, Mechanism of TRAIL-induced apoptosis. Huh7.5 cells were incubated with TRAIL following a 30-min preincubation with Bcl-2-, Bcl-xL-, and caspase inhibitors. Cells were incubated at a TRAIL concentration of 50 ng/ml (left panel), or for 2 h with different TRAIL concentrations (right panel). Inhibition of antiapoptotic Bcl-2 and Bcl-xL using dimethoxy-dihydro-dibenzodiazocine dioxide and dimethoxy-dinitrosobenzyl (50 μmol/l) showed a marked induction of TRAIL-induced apoptosis. C, After 10 min preincubation time with caspase-8 and -10 inhibitors Z-IETD-FMK (20 μmol/l) and Z-AEVD-FMK (20 μmol/l), cells were incubated for 2 h at a TRAIL concentration of 50 ng/ml. The inhibition of proapoptotic caspases-8 and -10 completely abolished TRAIL induced apoptosis (lanes 2 and 4), whereas inhibition of caspase-9 by inhibitor Z-LEHDFMK (20 μmol/l) markedly reduced apoptosis (lane 3).

FIGURE 2.

TRAIL-induced apoptosis in human Huh7.5 hepatoma cells is caspase and Bcl-2/Bcl-xL dependent. Following incubation with TRAIL, Huh7.5 cells were lysed and protein lysates were subjected to SDS-PAGE and Western blot analysis using anti-human PARP and caspase-specific Abs. A, left panel, Time course of TRAIL-induced apoptosis with PARP-, caspase-3, caspase-8, and caspase-9 cleavage following 1–24 h incubation with TRAIL at a concentration of 50 ng/ml. Right panel, Dose-dependent induction of TRAIL-dependent apoptosis at TRAIL concentrations ranging from 1 to 200 ng/ml (incubation time 2 h). B, Mechanism of TRAIL-induced apoptosis. Huh7.5 cells were incubated with TRAIL following a 30-min preincubation with Bcl-2-, Bcl-xL-, and caspase inhibitors. Cells were incubated at a TRAIL concentration of 50 ng/ml (left panel), or for 2 h with different TRAIL concentrations (right panel). Inhibition of antiapoptotic Bcl-2 and Bcl-xL using dimethoxy-dihydro-dibenzodiazocine dioxide and dimethoxy-dinitrosobenzyl (50 μmol/l) showed a marked induction of TRAIL-induced apoptosis. C, After 10 min preincubation time with caspase-8 and -10 inhibitors Z-IETD-FMK (20 μmol/l) and Z-AEVD-FMK (20 μmol/l), cells were incubated for 2 h at a TRAIL concentration of 50 ng/ml. The inhibition of proapoptotic caspases-8 and -10 completely abolished TRAIL induced apoptosis (lanes 2 and 4), whereas inhibition of caspase-9 by inhibitor Z-LEHDFMK (20 μmol/l) markedly reduced apoptosis (lane 3).

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Next, we aimed to study the mechanism of TRAIL-induced apoptosis in hepatocyte-derived cell lines. Using defined inhibitors targeting key mediators of distinct apoptotic pathways, we studied the functional impact of Bcl-2, Bcl-xL, and caspases for TRAIL-induced cell death. Proteins belonging to the Bcl-family are important modulators of mitochondrial apoptosis (38). As shown in Fig. 2,B specific inhibition of anti-apoptotic effectors Bcl-2 and Bcl-xL with dimethoxy-dihydro-dibenzodiazocine dioxide and dimethoxy-dinitrosobenzyl did not result in spontaneous apoptosis in Huh7.5 cells, but enhanced TRAIL-induced apoptosis. These data indicate that TRAIL-induced apoptosis is suppressed by Bcl-2 and Bcl-xL, but not completely inhibited. Hepatocytes belong to the so-called type-II cells that need mitochondrial amplification and consecutive caspase-9 activation for apoptosis induction via death ligands (39). In Huh7.5 cells, inhibition of caspase-9 with Z-LEHDFMK largely abolished TRAIL-induced apoptosis, as determined by cleaved PARP fragments (Fig. 2,C, lane 3). Inhibition of the initiator caspases-8 and -10 with Z-IETD-FMK and Z-AEVD-FMK, completely prevented TRAIL-induced apoptosis (lanes 2 and 4). In contrast, inhibition of caspase-3 only marginally altered TRAIL-induced apoptosis (see Fig. 8). These data demonstrate that TRAIL-induced apoptosis in Huh7.5 cells, similar to hepatocytes, largely depends on mitochondrial amplification. Furthermore, our data indicate that TRAIL-induced apoptosis is blocked if downstream signaling of the receptor-activated death-inducing signaling complex via caspase-8/-10 is inhibited.

FIGURE 8.

JFH1-dependent enhancement of TRAIL-induced apoptosis is caspase-9 dependent. Huh7.5 cells were transfected with HCV JFH1 or control GND RNA as described in Fig. 3. 72 h following transfection, cells were exposed to TRAIL in the presence or absence of caspase-inhibitors (see Fig. 2) and apoptosis was analyzed as described in Fig. 2. A, JFH1-dependent enhancement of TRAIL-induced apoptosis was completely inhibited by preincubation with pan-caspase inhibitor Z-VAD-FMK (Casp-Inh., 20 μM). B, JFH1-dependent enhancement of TRAIL-induced apoptosis was blocked by caspase-9 inhibitor Z-LEHD-FMK (Casp-9 Inh., 20 μM; lane 5), whereas JFH1-dependent enhancement of TRAIL-induced apoptosis (lane 4) was reduced by caspase-9 inhibitor to the level of JFH1 induced apoptosis (lane 2). C, Inhibition of caspase-3 using a caspase 3 inhibitor (2-(4-Methyl-8-(morpholin-4-ylsulfonyl)-1,3-dioxo-1,3-dihydro-2H-pyrrolo[3,4-c]quinolin-2-yl)ethyl- acetate; 10 μM) only marginally modified JFH1/TRAIL induced apoptosis as determined by analysis of cleaved PARP fragment. D, Preincubation of JFH-1 transfected Huh7.5 cells with Bcl-xL mimetic peptide (100 nM, 60 min) resulted in a minor reduction of TRAIL-dependent induction of apoptosis, as determined by analysis of cleaved PARP fragment.

FIGURE 8.

JFH1-dependent enhancement of TRAIL-induced apoptosis is caspase-9 dependent. Huh7.5 cells were transfected with HCV JFH1 or control GND RNA as described in Fig. 3. 72 h following transfection, cells were exposed to TRAIL in the presence or absence of caspase-inhibitors (see Fig. 2) and apoptosis was analyzed as described in Fig. 2. A, JFH1-dependent enhancement of TRAIL-induced apoptosis was completely inhibited by preincubation with pan-caspase inhibitor Z-VAD-FMK (Casp-Inh., 20 μM). B, JFH1-dependent enhancement of TRAIL-induced apoptosis was blocked by caspase-9 inhibitor Z-LEHD-FMK (Casp-9 Inh., 20 μM; lane 5), whereas JFH1-dependent enhancement of TRAIL-induced apoptosis (lane 4) was reduced by caspase-9 inhibitor to the level of JFH1 induced apoptosis (lane 2). C, Inhibition of caspase-3 using a caspase 3 inhibitor (2-(4-Methyl-8-(morpholin-4-ylsulfonyl)-1,3-dioxo-1,3-dihydro-2H-pyrrolo[3,4-c]quinolin-2-yl)ethyl- acetate; 10 μM) only marginally modified JFH1/TRAIL induced apoptosis as determined by analysis of cleaved PARP fragment. D, Preincubation of JFH-1 transfected Huh7.5 cells with Bcl-xL mimetic peptide (100 nM, 60 min) resulted in a minor reduction of TRAIL-dependent induction of apoptosis, as determined by analysis of cleaved PARP fragment.

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Because many viruses including cytomegalovirus (40), influenza virus (41), and adenovirus (42) have been shown to sensitize infected cells to TRAIL-induced apoptosis, we aimed to study whether HCV infection sensitizes Huh7.5 cells to TRAIL-induced apoptosis. To address this issue, we first exposed Huh7.5 cells harboring replicating infectious HCV to TRAIL. Compared with control transfected cells, replicating infectious HCV RNA markedly and significantly enhanced TRAIL-induced apoptosis, as shown by cleaved PARP (Fig. 3, A–D) and cleaved caspase-8 and -9 (Fig. 3, E and F). At the single-cell level, the TUNEL assay showed a statistically significant enhancement of TRAIL-induced apoptosis in cells harboring replicating HCV RNA, but not in the control cells harboring replication-deficient control RNA (Fig. 3, G and H). These results demonstrate that HCV sensitizes its host cells to TRAIL-induced apoptosis.

FIGURE 3.

HCV replication sensitizes Huh7.5 cells to TRAIL-induced apoptosis. Huh7.5 cells were transfected with JFH1 RNA or replication-deficient control RNA (GND). Seventy-two hours later, TRAIL was added and cells were incubated for an additional time period as indicated. Following cell lysis, apoptosis was assessed by immunoblot analysis of cleaved PARP, caspase-8, -9, and TUNEL assay. A and C, Analysis of apoptosis of Huh7.5 cells by immunoblot analysis of cleaved PARP. In the experiment depicted in A, cells were incubated with TRAIL (50 ng/ml) for 0–24 h. In the experiment depicted in C, cells were incubated for 6 h with increasing concentrations of TRAIL (0–200 ng/ml). B and D, Quantification of apoptosis by optical densitometric analysis of cleaved PARP levels relative to β-actin levels. Means ± SEM from three independent experiments are shown. E and F, Analysis of apoptosis of Huh7.5 cells incubated with TRAIL (50 ng/ml) for 6 h by immunoblot analysis of cleaved caspase-8 (E) and caspase-9 (F) as described in Fig. 2. G, Analysis of apoptosis of Huh7.5 cells by TUNEL assay as described in Materials and Methods. Immunostaining of HCV core protein (red, Cy3) and TUNEL reaction (green, FITC) were performed as described in Materials and Methods. H, Quantification of TUNEL-positive cells in HCV RNA-transfected Huh7.5 cells. Data are shown as mean percentage of TUNEL-positive cells divided by all cells (n = 500 corresponding to 100%); error bars represent SEM of three independent experiments; *, statistically significant vs JFH1 (Student’s t test, p < 0.05).

FIGURE 3.

HCV replication sensitizes Huh7.5 cells to TRAIL-induced apoptosis. Huh7.5 cells were transfected with JFH1 RNA or replication-deficient control RNA (GND). Seventy-two hours later, TRAIL was added and cells were incubated for an additional time period as indicated. Following cell lysis, apoptosis was assessed by immunoblot analysis of cleaved PARP, caspase-8, -9, and TUNEL assay. A and C, Analysis of apoptosis of Huh7.5 cells by immunoblot analysis of cleaved PARP. In the experiment depicted in A, cells were incubated with TRAIL (50 ng/ml) for 0–24 h. In the experiment depicted in C, cells were incubated for 6 h with increasing concentrations of TRAIL (0–200 ng/ml). B and D, Quantification of apoptosis by optical densitometric analysis of cleaved PARP levels relative to β-actin levels. Means ± SEM from three independent experiments are shown. E and F, Analysis of apoptosis of Huh7.5 cells incubated with TRAIL (50 ng/ml) for 6 h by immunoblot analysis of cleaved caspase-8 (E) and caspase-9 (F) as described in Fig. 2. G, Analysis of apoptosis of Huh7.5 cells by TUNEL assay as described in Materials and Methods. Immunostaining of HCV core protein (red, Cy3) and TUNEL reaction (green, FITC) were performed as described in Materials and Methods. H, Quantification of TUNEL-positive cells in HCV RNA-transfected Huh7.5 cells. Data are shown as mean percentage of TUNEL-positive cells divided by all cells (n = 500 corresponding to 100%); error bars represent SEM of three independent experiments; *, statistically significant vs JFH1 (Student’s t test, p < 0.05).

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Next, we aimed to confirm whether sensitization to TRAIL-induced apoptosis was also present in HCV-infected cells. Thus, we incubated Huh7.5 cells with HCVcc or noninfectious control supernatants derived from cells transfected with JFH1/ΔE1E2 RNA unable to produce infectious viral particles. As shown in Fig. 4, exposure of HCV-infected Huh7.5 cells to TRAIL resulted in enhanced apoptosis compared to control cells incubated with control supernatants, as shown by cleaved PARP (Fig. 4,A). Compared to viral genome delivery by electroporation, HCV-dependent enhancement of TRAIL-induced apoptosis appeared to be lower in HCV JFH1-infected hepatoma cells (as shown by the lower level of cleaved PARP when comparing Fig. 4,A to 3C). This difference was most likely due to low levels of MOI (TCID50 of 104/ml for HCVcc derived from HCV-JFH1 strain) resulting in less efficient delivery of the viral genome in infection compared with transfection experiments. To address this issue, we repeated infection experiments using infectious particles of viral strain Jc1 (J6-JFH1). In contrast to the JFH1 strain, the Jc1 isolate has been shown to allow production of virus with markedly enhanced infectivity (24). Indeed, infection of Huh7.5 cells at higher MOIs with HCV virions derived from the HCV Jc1 strain (TCID50 of 105–106/ml) resulted in similar enhancement of TRAIL-induced apoptosis as seen in transfection experiments (compare Fig. 4 C with 3C).

FIGURE 4.

Enhancement of TRAIL-induced apoptosis during HCV infection. Infection of PHH and Huh7.5 cells with HCV JFH1 TCID50 of 104/ml and HCV Jc1 TCID50 of 106/ml was performed by incubation of cells for 3 h with tissue culture supernatants of JFH1, Jc1, or JFH1/ΔE1E2 transfected Huh7.5 cells as described previously (1922 ). Seventy-two hours postinfection, TRAIL was added at the concentrations indicated. Six hours following the addition of TRAIL, apoptosis was analyzed by immunoblot analysis of cleaved PARP as described in Fig. 3. HCV infection was confirmed by immunoblot analysis of HCV NS5A expression. A, Immunoblot analysis of cleaved PARP in JFH1 infected Huh7.5 cells vs JFH1/ΔE1E2 control cells. B, Densitometric analysis of cleaved PARP from three independent experiments (see Fig. 3) showed that JFH1 infection significantly enhanced TRAIL-induced apoptosis as compared with incubation with noninfectious control supernatants (JFH1/ΔE1E2). C, Immunoblot analysis of cleaved PARP in Jc1-infected Huh7.5 cells vs JFH1/ΔE1E2 control cells. Cells were incubated in presence or absence of 100 ng/ml TRAIL for 2 h. D, Immunoblot analysis of cleaved PARP in JFH1-infected PHH and Huh7.5 vs JFH1/ΔE1E2 control cells. TRAIL (50 ng/ml, 6 h) induced cleavage of PARP only in HCV infected PHH (lane 2), but not in JFH1/ΔE1E2 control (lane 4). *, Statistically significant using Student’s t test (p < 0.05).

FIGURE 4.

Enhancement of TRAIL-induced apoptosis during HCV infection. Infection of PHH and Huh7.5 cells with HCV JFH1 TCID50 of 104/ml and HCV Jc1 TCID50 of 106/ml was performed by incubation of cells for 3 h with tissue culture supernatants of JFH1, Jc1, or JFH1/ΔE1E2 transfected Huh7.5 cells as described previously (1922 ). Seventy-two hours postinfection, TRAIL was added at the concentrations indicated. Six hours following the addition of TRAIL, apoptosis was analyzed by immunoblot analysis of cleaved PARP as described in Fig. 3. HCV infection was confirmed by immunoblot analysis of HCV NS5A expression. A, Immunoblot analysis of cleaved PARP in JFH1 infected Huh7.5 cells vs JFH1/ΔE1E2 control cells. B, Densitometric analysis of cleaved PARP from three independent experiments (see Fig. 3) showed that JFH1 infection significantly enhanced TRAIL-induced apoptosis as compared with incubation with noninfectious control supernatants (JFH1/ΔE1E2). C, Immunoblot analysis of cleaved PARP in Jc1-infected Huh7.5 cells vs JFH1/ΔE1E2 control cells. Cells were incubated in presence or absence of 100 ng/ml TRAIL for 2 h. D, Immunoblot analysis of cleaved PARP in JFH1-infected PHH and Huh7.5 vs JFH1/ΔE1E2 control cells. TRAIL (50 ng/ml, 6 h) induced cleavage of PARP only in HCV infected PHH (lane 2), but not in JFH1/ΔE1E2 control (lane 4). *, Statistically significant using Student’s t test (p < 0.05).

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Huh7.5 cells are derived from transformed hepatoma cells and therefore only represent a surrogate model for the natural target cell for HCV infection, the PHH. To confirm the relevance of our findings for the natural target cell of HCV infection, we reproduced key findings in HCV-infected primary human hepatocytes in contrast to Huh7.5 hepatoma cells have been shown to be resistant to TRAIL-induced apoptosis (5, 6, 7). Indeed, incubation of PHH with TRAIL did not yield in cleavage of PARP (Fig. 4,D, lane 4). Whereas incubation of PHH with TRAIL did not result in apoptosis (Fig. 4,D, lane 5), HCV JFH1 infection rendered PHH sensitive to TRAIL-induced apoptosis (Fig. 4,D, lane 2). Infection of PHH with HCV JFH1 was confirmed by detection of HCV NS5A protein expression in infected cells using immunoblot (Fig. 4 D, lanes 2 and 3). These results confirm the relevance of HCV-dependent sensitization to TRAIL-induced apoptosis for PHH.

Interestingly, we observed that transfection of Huh7.5 cells with replication-competent full-length HCV RNA itself can result in apoptosis of Huh7.5 cells as indicated by an increase in the number of TUNEL-positive cells compared with control cells (Fig. 3,H) and a low-level induction of cleaved PARP using immunoblots with long exposure times (see Fig. 8,B, lane 2 and data not shown). However, the induction of apoptosis by the virus itself was less pronounced when compared with apoptosis after exposure to TRAIL in cells harboring replicating HCV (compare lanes 1 and 5 in Fig. 4,A; lanes 2 and 4 in Fig. 3, E and F; lanes 2 and 4 see in Fig. 8 B).

To identify the viral factor(s) enhancing TRAIL-induced apoptosis, we determined whether HCV structural protein expression is required for HCV-TRAIL interaction. To address this question, we transfected Huh7.5 cells with a JFH1 variant (JFH1/ΔE1E2) containing a deletion in the HCV envelope coding region preventing synthesis of infectious viral particles. Because the phenotype of full-length JFH1 RNA and JFH1/ΔE1E2 was indistinguishable in regard to enhancement of TRAIL-induced apoptosis (Fig. 5), we conclude that HCV-TRAIL interaction is independent of production of infectious viral particles. To determine whether structural proteins are required for HCV-enhanced TRAIL-induced apoptosis, we compared the full-length JFH1 RNA with a JFH1 subgenomic replicon lacking the HCV core-NS2 region. Because the JFH1 subgenomic replicon exhibited a similar apoptosis phenotype as full-length JFH1 RNA (Fig. 5), we conclude that the expression of HCV structural proteins and NS2 is not required for enhancement of TRAIL induced apoptosis and sensitization to TRAIL-induced apoptosis is mediated predominantly by the HCV nonstructural proteins.

FIGURE 5.

Enhancement of TRAIL-induced apoptosis during HCV infection is independent of HCV structural protein expression and viral RNA. A, Huh7.5 cells were transfected with HCV JFH1 RNA derived from constructs described in Fig. 1. Seventy-two hours following transfection, cells were exposed to TRAIL at a concentration of 50 ng/ml for 2 h and apoptosis was analyzed as described in Fig. 3. B, Densitometric analysis of cleaved PARP (see Fig. 3) from three independent experiments indicated a similar enhancement of TRAIL-apoptosis by JFH1 full-length and subgenomic replicons.

FIGURE 5.

Enhancement of TRAIL-induced apoptosis during HCV infection is independent of HCV structural protein expression and viral RNA. A, Huh7.5 cells were transfected with HCV JFH1 RNA derived from constructs described in Fig. 1. Seventy-two hours following transfection, cells were exposed to TRAIL at a concentration of 50 ng/ml for 2 h and apoptosis was analyzed as described in Fig. 3. B, Densitometric analysis of cleaved PARP (see Fig. 3) from three independent experiments indicated a similar enhancement of TRAIL-apoptosis by JFH1 full-length and subgenomic replicons.

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Following mapping the viral factors required for HCV-dependent enhancement of TRAIL-induced apoptosis, we investigated the cellular factors mediating this HCV-host interaction. Several studies pointed to an involvement of TRAIL in apoptosis of virus-infected hepatocytes by an autocrine loop (43, 44). To address this question, we studied the impact of HCV replication and protein expression on the expression of TRAIL and TRAIL receptor R1 in Huh7.5 cells. As shown in Fig. 6, cells harboring replicating infectious HCV RNA showed decreased expression levels of TRAIL and unchanged levels of TRAIL receptor R1 expression when compared with cells transfected with replication-deficient viral control RNA. In contrast to PHH, TRAIL receptor R2 was not expressed in Huh7.5 cells (Fig. 6,C). Similar to Huh7.5 cells, infection of PHH with HCV JFH1 neither increased TRAIL expression, nor enhanced protein expression of TRAIL receptors R1, respectively (Fig. 6 D). These data suggest that a TRAIL autocrine loop does not play a major role for TRAIL-induced sensitization in this HCV infectious tissue culture system.

FIGURE 6.

JFH1-induced apoptosis in primary human hepatocytes and Huh7.5 cells is independent of TRAIL and TRAIL-R1/-R2 expression. PHH and Huh7.5 cells were infected/transfected with virions/RNA derived from constructs depicted in Fig. 1. Seventy-two hours postinfection/transfection, TRAIL, TRAIL-receptor R1, and TRAIL-receptor R2 expression were analyzed by Western blot analysis using specific Abs directed against human TRAIL, TRAIL-R1, and TRAIL-R2. A, Transfection of Huh7.5 cells with JFH1 RNA resulted in suppression of TRAIL expression, as compared with Huh7.5 cells transfected with replication-deficient GND RNA. B and C, Absent modulation of TRAIL-R1 expression and lacking TRAIL R2 expression in HCV JFH1 transfected Huh7.5 cells. Immunoblot analyses of naive PHH are shown as positive controls for TRAIL-R1 and TRAIL-R2 expression (lane 1). D, Down-regulation of TRAIL expression in PHH infected with HCV JFH1. Expression of TRAIL-R1 and TRAIL-R2 in PHH infected with HCV JFH1.

FIGURE 6.

JFH1-induced apoptosis in primary human hepatocytes and Huh7.5 cells is independent of TRAIL and TRAIL-R1/-R2 expression. PHH and Huh7.5 cells were infected/transfected with virions/RNA derived from constructs depicted in Fig. 1. Seventy-two hours postinfection/transfection, TRAIL, TRAIL-receptor R1, and TRAIL-receptor R2 expression were analyzed by Western blot analysis using specific Abs directed against human TRAIL, TRAIL-R1, and TRAIL-R2. A, Transfection of Huh7.5 cells with JFH1 RNA resulted in suppression of TRAIL expression, as compared with Huh7.5 cells transfected with replication-deficient GND RNA. B and C, Absent modulation of TRAIL-R1 expression and lacking TRAIL R2 expression in HCV JFH1 transfected Huh7.5 cells. Immunoblot analyses of naive PHH are shown as positive controls for TRAIL-R1 and TRAIL-R2 expression (lane 1). D, Down-regulation of TRAIL expression in PHH infected with HCV JFH1. Expression of TRAIL-R1 and TRAIL-R2 in PHH infected with HCV JFH1.

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The mitochondrial apoptosis pathway has been shown to play an important role in virus-induced modulations of host cell apoptosis (10). As shown in Fig. 2, we have already demonstrated that TRAIL-induced apoptosis in Huh7.5 cells is enhanced following the inhibition of Bcl-2/Bcl-xL (Fig. 2,B). We therefore analyzed Bcl-2 and Bcl-xL protein expression in JFH1-transfected Huh7.5 cells and JFH1 infected PHH. Interestingly, expression of Bcl-2 and Bcl-xL was suppressed in JFH1-transfected Huh7.5 cells (Fig. 7, A and B). These results were confirmed in PHH, where infection with JFH1 appeared to reduce Bcl-2 and Bcl-xL protein expression (Fig. 7,C). The less pronounced reduction in Bcl-2/Bcl-xL protein expression observed in PHH (Fig. 7,C) compared with Huh7.5 cells (Fig. 7, A and B) may be due to the lower number of cells containing replicating HCV in PHH. Incubation of Huh7.5 with a Bcl-xL mimetic peptide resulted in a small but reproducible reduction (three of three experiments) of TRAIL-induced apoptosis in JFH1 replicating cells (Fig. 8,D). Mitochondrial-dependent apoptosis is characterized by release of mitochondrial cytochrome C into the cytoplasm (11, 12). We therefore studied cytochrome C levels in cytosolic fractions and fractions containing the mitochondria of HCV JFH1-replicating Huh7.5 cells incubated with TRAIL. As shown in Fig. 7,D, hepatoma cells with replicating virus and treated with TRAIL demonstrated an increase of cytochrome C levels in cytosolic fractions compared with control-transfected cells incubated with TRAIL. These data suggest that cytochrome C appears to be released from the mitochondria in TRAIL-treated cells with replicating HCV and suggests that the mitochondrial pathway contributes to HCV-induced enhancement of TRAIL-induced apoptosis. Interestingly, HCV replication alone also resulted in release of cytochrome C into the cytosol (Fig. 7,D) consistent with low level induction of apoptosis by JFH1 itself (see Fig. 3 H).

FIGURE 7.

HCV JFH1 replication suppresses antiapoptotic Bcl-2 and Bcl-xL expression. Huh7.5 cells and PHH were transfected/infected with HCV RNA/virions derived from constructs depicted in Fig. 1. Seventy-two hours post transfection, Bcl-2 (A and C), Bcl-xL (B and C) expression and cytochrome C levels in cellular subfractions (D) were analyzed by Western blot analysis. Suppression of antiapoptotic Bcl-2 (A) and antiapoptotic Bcl-xL (B) protein expression in JFH1-transfected Huh7.5 cells compared with Huh7.5 cells transfected with replication-deficient HCV GND RNA was detectable. C, Bcl-2 and Bcl-xL protein expression in PHH infected with HCV JFH1. PHH were infected with HCV JFH1 as described in Fig. 4 and Bcl-2 and Bcl-xL protein expression was detected 72 h following infection as described above. D, Cytochrome C levels in subcellular fractions (cytosolic and organelle fractions containing mitochondria) of cells with replicating virus incubated with or without TRAIL. Cells were transfected with JFH1 RNA or replication-deficient control RNA (GND). Seventy-two hours post transfection TRAIL was added (100 ng/ml) for 2 h. Following lysis and cellular fractionation of cell lysates as described in Materials and Methods, cytochrome C levels in cellular subfractions were analyzed by immunoblot using anti-cytochrome C specific Ab. Cyt, cytosolic subfraction; Org, organelle subfraction containing mitochondria.

FIGURE 7.

HCV JFH1 replication suppresses antiapoptotic Bcl-2 and Bcl-xL expression. Huh7.5 cells and PHH were transfected/infected with HCV RNA/virions derived from constructs depicted in Fig. 1. Seventy-two hours post transfection, Bcl-2 (A and C), Bcl-xL (B and C) expression and cytochrome C levels in cellular subfractions (D) were analyzed by Western blot analysis. Suppression of antiapoptotic Bcl-2 (A) and antiapoptotic Bcl-xL (B) protein expression in JFH1-transfected Huh7.5 cells compared with Huh7.5 cells transfected with replication-deficient HCV GND RNA was detectable. C, Bcl-2 and Bcl-xL protein expression in PHH infected with HCV JFH1. PHH were infected with HCV JFH1 as described in Fig. 4 and Bcl-2 and Bcl-xL protein expression was detected 72 h following infection as described above. D, Cytochrome C levels in subcellular fractions (cytosolic and organelle fractions containing mitochondria) of cells with replicating virus incubated with or without TRAIL. Cells were transfected with JFH1 RNA or replication-deficient control RNA (GND). Seventy-two hours post transfection TRAIL was added (100 ng/ml) for 2 h. Following lysis and cellular fractionation of cell lysates as described in Materials and Methods, cytochrome C levels in cellular subfractions were analyzed by immunoblot using anti-cytochrome C specific Ab. Cyt, cytosolic subfraction; Org, organelle subfraction containing mitochondria.

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Next, we aimed to study the impact of caspases for HCV-mediated enhancement of TRAIL-induced apoptosis. Several forms of apoptosis do not involve caspase activation. We therefore inhibited caspases with the pan-caspase inhibitor Z-VAD-FMK. Interestingly, inhibition of caspases completely abolished JFH1-dependent enhancement of TRAIL-induced apoptosis (Fig. 8,A, lane 3 and Fig. 9,C, lane 3). These findings indicate an essential role of caspase activation for TRAIL-induced apoptosis of Huh7.5 cells harboring replicating HCV. To determine the impact of mitochondrial apoptosis in HCV infection, we inhibited the mitochondrial-activated caspase-9. Inhibition of caspase-9 with Z-LEHD-FMK largely blocked JFH1-dependent enhancement of TRAIL-induced apoptosis (Fig. 8,B, lane 5), suggesting an essential contribution of the mitochondrial apoptotic pathway for HCV-mediated sensitization of TRAIL-induced apoptosis. Interestingly, inhibition of caspase-3 only marginally affected TRAIL-induced apoptosis in JFH1 replicating Huh7.5 cells (Fig. 8 C) indicating that caspase-3 does not play a major role in execution of TRAIL/HCV apoptosis.

FIGURE 9.

TRAIL-induced apoptosis in HCV-replicating cells induces down-regulation of viral protein expression. Huh7.5 cells were transfected with HCV JFH1 or control GND RNA as described in Fig. 2. Seventy-two hours following transfection, cells were exposed to TRAIL in the presence or absence of the pan-caspase inhibitor Z-VAD-FMK (Fig. 2) and apoptosis was analyzed as described in Fig. 2. HCV core and E2 protein expression were analyzed by immunoblot. A, TRAIL (50 ng/ml) induces apoptosis as shown by cleaved PARP and results in down-regulation of HCV core and E2 protein expression. TRAIL incubation time is indicated above the lanes. B, Densitometric analysis of HCV core and E2 levels demonstrated statistically significant reduction of HCV core and E2 protein expression 6 and 24 h following incubation with TRAIL, respectively. C and D, Inhibition of TRAIL-induced apoptosis by preincubation with pan-caspase-inhibitor Z-VAD-FMK (20 μmol/l) restores HCV E2 and core protein expression as shown by immunoblot (C) and densitometric analysis of HCV core and E2 protein levels (D). Data are presented as mean values of three independent experiments ± SEM. *, Statistically significant using Student’s t test, p < 0.05. E, Analysis of HCV protein expression in apoptotic cells by immunofluorescence. Huh7.5 cells were transfected with JFH1 RNA as described in Fig. 1 stained with a monoclonal anti-HCV core Ab (red) and counterstained with DAPI (blue) for visualization of cell nuclei. Apoptosis was determined by TUNEL assay (green) as described in Materials and Methods. Single cell analysis using confocal laser scanning microscopy revealed reduced HCV core expression levels in apoptotic Huh7.5 cells compared with nonapoptotic cells expressing HCV proteins. Cells with replicating HCV without apoptosis are indicated by long, thin arrows, apoptotic (TUNEL positive) cells with replicating HCV are indicated by short, thick arrows.

FIGURE 9.

TRAIL-induced apoptosis in HCV-replicating cells induces down-regulation of viral protein expression. Huh7.5 cells were transfected with HCV JFH1 or control GND RNA as described in Fig. 2. Seventy-two hours following transfection, cells were exposed to TRAIL in the presence or absence of the pan-caspase inhibitor Z-VAD-FMK (Fig. 2) and apoptosis was analyzed as described in Fig. 2. HCV core and E2 protein expression were analyzed by immunoblot. A, TRAIL (50 ng/ml) induces apoptosis as shown by cleaved PARP and results in down-regulation of HCV core and E2 protein expression. TRAIL incubation time is indicated above the lanes. B, Densitometric analysis of HCV core and E2 levels demonstrated statistically significant reduction of HCV core and E2 protein expression 6 and 24 h following incubation with TRAIL, respectively. C and D, Inhibition of TRAIL-induced apoptosis by preincubation with pan-caspase-inhibitor Z-VAD-FMK (20 μmol/l) restores HCV E2 and core protein expression as shown by immunoblot (C) and densitometric analysis of HCV core and E2 protein levels (D). Data are presented as mean values of three independent experiments ± SEM. *, Statistically significant using Student’s t test, p < 0.05. E, Analysis of HCV protein expression in apoptotic cells by immunofluorescence. Huh7.5 cells were transfected with JFH1 RNA as described in Fig. 1 stained with a monoclonal anti-HCV core Ab (red) and counterstained with DAPI (blue) for visualization of cell nuclei. Apoptosis was determined by TUNEL assay (green) as described in Materials and Methods. Single cell analysis using confocal laser scanning microscopy revealed reduced HCV core expression levels in apoptotic Huh7.5 cells compared with nonapoptotic cells expressing HCV proteins. Cells with replicating HCV without apoptosis are indicated by long, thin arrows, apoptotic (TUNEL positive) cells with replicating HCV are indicated by short, thick arrows.

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If HCV replication enhances TRAIL-induced apoptosis, HCV-infected cells should preferentially be eliminated. To address this question, we studied the impact of TRAIL on HCV protein expression in Huh7.5 cells and PHH harboring replicating infectious HCV. In line with this hypothesis, incubation of HCV JFH1 RNA-transfected Huh7.5 cells with TRAIL induced a significant reduction of HCV core and E2 protein expression (Fig. 9, A and B). As shown in immunofluorescence analyses of individual cells, apoptotic Huh7.5 cells with replicating HCV exhibited a marked reduction of HCV core protein expression compared with nonapoptotic cells (Fig. 9,E). These results were further confirmed by quantification of viral protein expression using immunofluorescence analyses (data not shown). Similar to findings in Huh7.5 hepatoma cells, incubation with TRAIL appeared to result in suppression of HCV protein expression in HCV JFH1-infected PHH (Fig. 4,D, lane 2, compared with lane 3). The TRAIL-dependent reduction of HCV protein expression was dependent on apoptosis induction, since preincubation of Huh7.5 cells with pan-caspase-inhibitor Z-VAD-FMK completely restored expression of the HCV proteins core and E2, respectively (Fig. 9, C and D). These findings confirm the impact of TRAIL-induced apoptosis for HCV-host interactions and suggest that sensitization to TRAIL-induced apoptosis in cells harboring replicating HCV may contribute to control of HCV infection.

In this study, we demonstrate that HCV infection sensitizes the host cell to TRAIL-induced apoptosis. Detailed analyses indicate that this sensitization depends on the expression of HCV nonstructural proteins NS3, NS4, and NS5. The interaction between HCV and the host cell apoptosis machinery involves caspase-9 and the mitochondrial but not the autocrine TRAIL-mediated pathway. Furthermore, we show that induction of apoptosis by TRAIL results in suppression of HCV protein expression, suggesting that this mechanism may contribute to elimination of HCV-infected hepatocytes.

For virtually all HCV proteins, interference with apoptosis has been reported. However, most studies assessing the effect of HCV infection on cellular apoptosis were performed in surrogate models of HCV infection, e.g., stably transfected cell lines or recombinant proteins expressed in heterologous systems. Thus, the relevance of these observations for HCV infection remained uncertain. Limitations of surrogate models of HCV infection are, among others, artificially elevated levels of expressed HCV proteins or the lack of genetic elements in subgenomic replicons (13). To overcome these limitations and to study the mechanism of apoptosis in a model system closer to HCV infection in vivo, we used the HCVcc model system allowing us to study HCV-host interactions in the context of the complete viral life cycle (18, 19, 20).

In contrast to our observations having identified the HCV nonstructural proteins as key players for sensitization of TRAIL-induced apoptosis, a recent study had demonstrated an important role of core for TRAIL-induced apoptosis (31). Isolate-specific factors (genotype 1 vs 2), different core protein levels in the infectious cell culture system (this study), and transfection of cDNA (31), as well as a dominant effect of nonstructural proteins in the infectious tissue culture system, may explain the different findings. Using a full-length replicon expressing all viral proteins, Lee et al. showed that HCV envelope glycoprotein E2 can antagonize the proapoptotic effects of HCV core protein (32). In our study, we did not observe a markedly altered induction of apoptosis in cells transfected with HCV RNA containing a deletion in the HCV envelope proteins (JFH1/ΔE1E2) compared with infectious HCV RNA (Fig. 5). Because subgenomic replicons still showed sensitization to TRAIL-induced apoptosis (Fig. 5), our findings clearly demonstrate that the HCV structural proteins may not represent the major factors that determine sensitization to TRAIL, although we cannot exclude a modulatory role of these proteins. Because subgenomic replicons lack a functional NS2 protein (Fig. 1), our data also suggest that the recently described antiapoptotic effect of NS2 in a transgenic mouse model (45) may not play a major role in the enhancement of TRAIL-induced apoptosis in HCV-infected human hepatoma cells.

Interestingly, proapoptotic effects of HCV nonstructural proteins NS3/NS4A (46, 47) and NS5A (48) have been described recently. NS3 complexed with NS4A is known to translocate to the mitochondrial membrane where direct apoptosis induction, independent from caspase-8 activation can occur (47). Conversely, NS3 has been described to cleave Cardif and thereby act antiapoptotically (49). NS5A has recently been shown to directly inhibit proapoptotic Bin1, a tumor suppressor protein with a SH3 domain, thereby facilitating apoptosis induction in hepatoma cells (48). By contrast, NS5A has sequence homologies with Bcl-2 and binds to FKBP38, thereby augmenting the antiapoptotic effect of Bcl-2 (50) and inhibiting the proapoptotic action of Bax in hepatoma cells (51). Although we cannot exclude a functional relevance of antiapoptotic properties of NS5A and NS3, our data suggest that these effects are not able to override the proapoptotic effects of the HCV nonstructural proteins mediating sensitization to TRAIL.

The proapoptotic effects of both NS3/NS4A (47) and NS5A (48) have been described to converge at the level of the mitochondria and may explain the proapoptotic properties of HCV infection. In this study, we demonstrate evidence that HCV-induced enhancement of Huh7.5 cell apoptosis depends on the mitochondrial pathway, because HCV replication induced cytochrome C release into the cytosol (Fig. 7,D) and inhibition of caspase-9 markedly abolished HCV-dependent TRAIL-induced apoptosis (Fig. 8). The cytochrome C release may be partly due to the HCV-induced down-regulation of Bcl-2 and Bcl-xL expression (Fig. 7, A–C). Furthermore, preincubation with a Bcl-xL mimetic peptide resulted in a small but reproducible (three of three experiments) reduction of HCV-dependent apoptosis as shown in Fig. 8,D. Bcl-2 and Bcl-xL are known to be essential in inhibiting mitochondrial apoptosis (12). In line with these results, a recent study demonstrated down-regulation of antiapoptotic Bcl-2 and up-regulation of proapoptotic PUMA and Bax in freshly prepared liver sections of HCV-infected patients (5). HCV nonstructural proteins have been shown to induce ER stress (52), and Bcl-2-/Bcl-xL-dependent transmission of ER-stress into a mitochondrial apoptotic signal has recently been demonstrated (38). Nevertheless, down-regulation of Bcl-2 and Bcl-xL alone seems not sufficient to explain proapoptotic properties of HCV JFH1, as inhibition of both proteins did not enhance TRAIL-induced apoptosis to the extent of the HCV-dependent apoptosis (Fig. 2), and incubation of cells with a Bcl-xL mimetic peptide was only partially modulating TRAIL-induced apoptosis in Huh7.5 cells (Fig. 8 D).

Several authors have postulated an autocrine TRAIL-dependent apoptosis of hepatocytes. Mundt et al. (43) demonstrated TRAIL-dependent apoptosis in hepatocytes using an adenoviral vector expressing TRAIL, and Volkmann et al. (5) described an up-regulation of TRAIL receptors in HCV-infected human liver sections. Most recently, HCV-dependent up-regulation of TRAIL and apoptosis induction in a novel hepatoma cell line has been described (44). In the latter study, apoptosis of hepatoma cells was dependent on autologous TRAIL expression and HCV-dependent apoptosis resulted in death of all infected cells. In contrast, in PHH and Huh7.5 cells harboring replicating HCV, we observed a decreased expression of TRAIL and unchanged TRAIL receptor expression levels (Fig. 6). Moreover, immunohistochemistry of TRAIL expression in the HCV-infected liver revealed that nonparenchymal mononuclear cells, but not hepatocytes, appear to be predominant producers of TRAIL (data not shown). Taken together, these data suggest that a TRAIL autocrine loop does not play a major role for TRAIL-induced sensitization.

Although HCV replication resulted in detectable induction of apoptosis (Fig. 3, A–H) and cytochrome C release (Fig. 7,D), this induction was significantly less efficient than sensitization to TRAIL-induced apoptosis (see side-by-side experiments depicted in Fig. 3, A–H and Fig. 8). Thus, it seems unlikely that induction of endogenous TRAIL production is responsible for the identified HCV-dependent enhancement of TRAIL-induced apoptosis in Huh7.5 cells. This hypothesis is in line with several studies demonstrating that sensitivity toward TRAIL-induced apoptosis does not correlate with TRAIL receptor expression of target cells (53, 54). Whether massive HCV-induced cell death described in another hepatoma cell line (44) or moderate virus-induced apoptosis in Huh7.5 cells in the classical HCV tissue culture model cell line Huh7.5 (our study; see Fig. 3 H and data not shown) more accurately reflects virus-host interactions during the natural course of HCV infection remains to be determined.

Apoptosis of virus-infected cells is a key mechanism of viral clearance in mammals (55). Moreover, several studies have pointed to a central role of TRAIL in the elimination of viruses via induction of host cell apoptosis (40, 41, 42, 43). In line with this concept, induction of hepatocyte apoptosis has been observed in the HCV-infected liver (4, 5). Confirming these findings, our own histopathological analyses demonstrated expression of TRAIL in CD8+ T cells and CD68+ macrophages in the immediate vicinity of apoptotic hepatocytes in the HCV infected liver (data not shown). Several studies have demonstrated TRAIL-dependent cell death by activated liver macrophages (30) and/or CD8+ T cells (40, 41, 56). Furthermore, elimination of influenza virus in mice has been shown to require TRAIL-expressing lymphocytes (41). In hepatits B virus (HBV) infection, lymphocyte-dependent hepatocyte apoptosis has been demonstrated to depend on TRAIL (56). Taken together, these data suggest a functional impact of TRAIL-expressing mononuclear cells, including T cells and macrophages in the elimination of virus-infected hepatocytes. Interestingly, a recent study has demonstrated that another hepatotropic virus, HBV, sensitizes hepatocytes to TRAIL-induced apoptosis via the Bcl-2 protein Bax (57), and in a mouse model of adenoviral hepatitis, TRAIL-mediated apoptosis was restrained by Bcl-xL (58). In line with these findings for other viruses including HBV, our results suggest that sensitization of TRAIL-induced apoptosis may play a key role in host antiviral defense mechanisms against HCV infection. This hypothesis is supported by our experimental finding that incubation of PHH and Huh7.5 cells with TRAIL resulted in a decrease of viral protein levels (Fig. 4, C and D; Fig. 9). This concept is further supported by the observation that IFN-dependent up-regulation of TRAIL on NK cells and macrophages seems crucial for elimination of viral infections (59). Furthermore, therapy of HCV-infected patients with pegylated IFN-α and ribavirin results in a rapid and sustained TRAIL elevation, suggesting a role of TRAIL in viral clearance (60).

Taken together, our results define a novel antiviral host defense mechanism which may play an important role for the control of HCV infection. HCV-induced TRAIL sensitization may have important implications for the pathogenesis of HCV infection and may contribute to the elimination of virus-infected hepatocytes.

We thank C. M. Rice for the gift of Huh7.5 cells, H. B. Greenberg and M. Houghton for the gift of Abs, and V. Lohmann for helpful discussions.

The authors have no financial conflict of interest.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1

This study was supported by the Fritz Thyssen Stiftung (to R.F.), Germany, the Faculty of Medicine, University of Freiburg, Germany (to R.F. and A.S.-G.), and by grants of Institut National de la Santé et de la Recherche Médicale, France, the European Union (LSHM-CT-2004–503359 “VIRGIL”; to T.F.B.), Belgium, the chair of excellence program of the Agence Nationale de la Recherche (ANR-05-CEXC-008; to T.F.B.), France, the Agence Nationale de la Recherche sur le SIDA et les Hépatites Virales (ANRS 06221; to T.F.B.), France, the Deutsche Forschungsgemeinschaft (Ba1417/11–2; to T.F.B.), Germany, France and the Else Kröner-Fresenius Foundation, Bad Homburg, Germany (P17/07//A83/06; T. F. B.). M.B.Z. was supported by the Inserm Poste Vert program in the framework of Institut National de la Santé et de la Recherche Médicale European Associated Laboratory Freiburg-Strasbourg. T.W. is supported by a grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science, from the Ministry of Health, Labour and Welfare of Japan and from the Ministry of Education, Culture, Sports, Science and Technology, and by the Research on Health Sciences Focusing on Drug Innovation from the Japan Health Sciences Foundation.

5

Abbreviations used in this paper: HCV, hepatitis C virus; PHH, primary human hepatocyte; MOI, multiplicity of infection; JFH1, Japanese fulminant hepatitis 1 isolate; HCVcc, cell culture-derived HCV; PARP, poly (ADP-ribose) polymerase; HBV, hepatitis B virus.

1
Lauer, G. M., B. D. Walker.
2001
. Hepatitis C virus infection.
N. Engl. J. Med.
345
:
41
-52.
2
Bartenschlager, R..
2006
. Hepatitis C virus molecular clones: from cDNA to infectious virus particles in cell culture.
Curr. Opin. Microbiol.
9
:
416
-422.
3
Dustin, L. B., C. M. Rice.
2007
. Flying under the radar: the immunobiology of hepatitis C.
Annu. Rev. Immunol.
25
:
71
-99.
4
Calabrese, F., P. Pontisso, E. Pettenazzo, L. Benvegnu, A. Vario, L. Chemello, A. Alberti, M. Valente.
2000
. Liver cell apoptosis in chronic hepatitis C correlates with histological but not biochemical activity or serum HCV-RNA levels.
Hepatology
31
:
1153
-1159.
5
Volkmann, X., U. Fischer, M. J. Bahr, M. Ott, F. Lehner, M. Macfarlane, G. M. Cohen, M. P. Manns, K. Schulze-Osthoff, H. Bantel.
2007
. Increased hepatotoxicity of tumor necrosis factor-related apoptosis-inducing ligand in diseased human liver.
Hepatology
46
:
1498
-1508.
6
LeBlanc, H. N., A. Ashkenazi.
2003
. Apo2L/TRAIL and its death and decoy receptors.
Cell Death Differ.
10
:
66
-75.
7
Lawrence, D., Z. Shahrokh, S. Marsters, K. Achilles, D. Shih, B. Mounho, K. Hillan, K. Totpal, L. DeForge, P. Schow, et al
2001
. Differential hepatocyte toxicity of recombinant Apo2L/TRAIL versions.
Nat. Med.
7
:
383
-385.
8
Zylberberg, H., A. C. Rimaniol, S. Pol, A. Masson, D. De Groote, P. Berthelot, J. F. Bach, C. Brechot, F. Zavala.
1999
. Soluble tumor necrosis factor receptors in chronic hepatitis C: a correlation with histological fibrosis and activity.
J. Hepatol.
30
:
185
-191.
9
Kumar, S..
2007
. Caspase function in programmed cell death.
Cell Death Differ.
14
:
32
-43.
10
Irusta, P. M., Y. B. Chen, J. M. Hardwick.
2003
. Viral modulators of cell death provide new links to old pathways.
Curr. Opin. Cell Biol.
15
:
700
-705.
11
Kakkar, P., B. K. Singh.
2007
. Mitochondria: a hub of redox activities and cellular distress control.
Mol. Cell. Biochem.
305
:
235
-253.
12
Armstrong, J. S..
2006
. The role of the mitochondrial permeability transition in cell death.
Mitochondrion
6
:
225
-234.
13
Fischer, R., T. Baumert, H. E. Blum.
2007
. Hepatitis C virus infection and apoptosis.
World J. Gastroenterol.
13
:
4865
-4872.
14
Der, S. D., Y. L. Yang, C. Weissmann, B. R. Williams.
1997
. A double-stranded RNA-activated protein kinase-dependent pathway mediating stress-induced apoptosis.
Proc. Natl. Acad. Sci. USA
94
:
3279
-3283.
15
Yoneyama, M., T. Fujita.
2007
. Function of RIG-I-like receptors in antiviral innate immunity.
J. Biol. Chem.
282
:
15315
-15318.
16
Zhang, P., C. E. Samuel.
2007
. Protein kinase PKR plays a stimulus- and virus-dependent role in apoptotic death and virus multiplication in human cells.
J. Virol.
81
:
8192
-8200.
17
Zeisel, M. B., T. F. Baumert.
2006
. Production of infectious hepatitis C virus in tissue culture: a breakthrough for basic and applied research.
J. Hepatol.
44
:
436
-439.
18
Lindenbach, B. D., M. J. Evans, A. J. Syder, B. Wolk, T. L. Tellinghuisen, C. C. Liu, T. Maruyama, R. O. Hynes, D. R. Burton, J. A. McKeating, C. M. Rice.
2005
. Complete replication of hepatitis C virus in cell culture.
Science
309
:
623
-626.
19
Wakita, T., T. Pietschmann, T. Kato, T. Date, M. Miyamoto, Z. Zhao, K. Murthy, A. Habermann, H. G. Krausslich, M. Mizokami, et al
2005
. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.
Nat. Med.
11
:
791
-796.
20
Zhong, J., P. Gastaminza, G. Cheng, S. Kapadia, T. Kato, D. R. Burton, S. F. Wieland, S. L. Uprichard, T. Wakita, F. V. Chisari.
2005
. Robust hepatitis C virus infection in vitro.
Proc. Natl. Acad. Sci. USA
102
:
9294
-9299.
21
Codran, A., C. Royer, D. Jaeck, M. Bastien-Valle, T. F. Baumert, M. P. Kieny, C. A. Pereira, J. P. Martin.
2006
. Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis.
J. Gen. Virol.
87
:
2583
-2593.
22
Zeisel, M. B., G. Koutsoudakis, E. K. Schnober, A. Haberstroh, H. E. Blum, F. L. Cosset, T. Wakita, D. Jaeck, M. Doffoel, C. Royer, et al
2007
. Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81.
Hepatology
46
:
1722
-1731.
23
Kato, T., T. Date, M. Miyamoto, A. Furusaka, K. Tokushige, M. Mizokami, T. Wakita.
2003
. Efficient replication of the genotype 2a hepatitis C virus subgenomic replicon.
Gastroenterology
125
:
1808
-1817.
24
Pietschmann, T., A. Kaul, G. Koutsoudakis, A. Shavinskaya, S. Kallis, E. Steinmann, K. Abid, F. Negro, M. Dreux, F. L. Cosset, R. Bartenschlager.
2006
. Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras.
Proc. Natl. Acad. Sci. USA
103
:
7408
-7413.
25
Fischer, R., R. Reinehr, T. P. Lu, A. Schonicke, U. Warskulat, H. P. Dienes, D. Haussinger.
2005
. Intercellular communication via gap junctions in activated rat hepatic stellate cells.
Gastroenterology
128
:
433
-448.
26
Pestka, J. M., M. B. Zeisel, E. Blaser, P. Schurmann, B. Bartosch, F. L. Cosset, A. H. Patel, H. Meisel, J. Baumert, S. Viazov, et al
2007
. Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C.
Proc. Natl. Acad. Sci. USA
104
:
6025
-6030.
27
Burckstummer, T., M. Kriegs, J. Lupberger, E. K. Pauli, S. Schmittel, E. Hildt.
2006
. Raf-1 kinase associates with Hepatitis C virus NS5A and regulates viral replication.
FEBS Lett.
580
:
575
-580.
28
Barth, H., A. Ulsenheimer, G. R. Pape, H. M. Diepolder, M. Hoffmann, C. Neumann-Haefelin, R. Thimme, P. Henneke, R. Klein, G. Paranhos-Baccala, et al
2005
. Uptake and presentation of hepatitis C virus-like particles by human dendritic cells.
Blood
105
:
3605
-3614.
29
Steinmann, D., H. Barth, B. Gissler, P. Schurmann, M. I. Adah, J. T. Gerlach, G. R. Pape, E. Depla, D. Jacobs, G. Maertens, et al
2004
. Inhibition of hepatitis C virus-like particle binding to target cells by antiviral antibodies in acute and chronic hepatitis C.
J. Virol.
78
:
9030
-9040.
30
Fischer, R., A. Cariers, R. Reinehr, D. Haussinger.
2002
. Caspase 9-dependent killing of hepatic stellate cells by activated Kupffer cells.
Gastroenterology
123
:
845
-861.
31
Chou, A. H., H. F. Tsai, Y. Y. Wu, C. Y. Hu, L. H. Hwang, P. I. Hsu, P. N. Hsu.
2005
. Hepatitis C virus core protein modulates TRAIL-mediated apoptosis by enhancing Bid cleavage and activation of mitochondria apoptosis signaling pathway.
J. Immunol.
174
:
2160
-2166.
32
Lee, S. H., Y. K. Kim, C. S. Kim, S. K. Seol, J. Kim, S. Cho, Y. L. Song, R. Bartenschlager, S. K. Jang.
2005
. E2 of hepatitis C virus inhibits apoptosis.
J. Immunol.
175
:
8226
-8235.
33
Chiou, H. L., Y. S. Hsieh, M. R. Hsieh, T. Y. Chen.
2006
. HCV E2 may induce apoptosis of Huh-7 cells via a mitochondrial-related caspase pathway.
Biochem. Biophys. Res. Commun.
345
:
453
-458.
34
Sprick, M. R., E. Rieser, H. Stahl, A. Grosse-Wilde, M. A. Weigand, H. Walczak.
2002
. Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8.
EMBO J.
21
:
4520
-4530.
35
Wieder, T., F. Essmann, A. Prokop, K. Schmelz, K. Schulze-Osthoff, R. Beyaert, B. Dorken, P. T. Daniel.
2001
. Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3.
Blood
97
:
1378
-1387.
36
Tang, D., J. M. Lahti, V. J. Kidd.
2000
. Caspase-8 activation and bid cleavage contribute to MCF7 cellular execution in a caspase-3-dependent manner during staurosporine-mediated apoptosis.
J. Biol. Chem.
275
:
9303
-9307.
37
Oliver, F. J., G. de la Rubia, V. Rolli, M. C. Ruiz-Ruiz, G. de Murcia, J. M. Murcia.
1998
. Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant.
J. Biol. Chem.
273
:
33533
-33539.
38
Hetz, C. A..
2007
. ER Stress Signaling and the BCL-2 Family of Proteins: From Adaptation to Irreversible Cellular Damage.
Antioxid. Redox. Signal
9
:
2345
-2356.
39
Ozoren, N., W. S. El-Deiry.
2002
. Defining characteristics of Types I and II apoptotic cells in response to TRAIL.
Neoplasia
4
:
551
-557.
40
Strater, J., H. Walczak, T. Pukrop, L. Von Muller, C. Hasel, M. Kornmann, T. Mertens, P. Moller.
2002
. TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections.
Gastroenterology
122
:
659
-666.
41
Ishikawa, E., M. Nakazawa, M. Yoshinari, M. Minami.
2005
. Role of tumor necrosis factor-related apoptosis-inducing ligand in immune response to influenza virus infection in mice.
J. Virol.
79
:
7658
-7663.
42
Sedger, L. M., D. M. Shows, R. A. Blanton, J. J. Peschon, R. G. Goodwin, D. Cosman, S. R. Wiley.
1999
. IFN-γ mediates a novel antiviral activity through dynamic modulation of TRAIL and TRAIL receptor expression.
J. Immunol.
163
:
920
-926.
43
Mundt, B., F. Kuhnel, L. Zender, Y. Paul, H. Tillmann, C. Trautwein, M. P. Manns, S. Kubicka.
2003
. Involvement of TRAIL and its receptors in viral hepatitis.
FASEB J.
17
:
94
-96.
44
Zhu, H., H. Dong, E. Eksioglu, A. Hemming, M. Cao, J. M. Crawford, D. R. Nelson, C. Liu.
2007
. Hepatitis C virus triggers apoptosis of a newly developed hepatoma cell line through antiviral defense system.
Gastroenterology
133
:
1649
-1659.
45
Erdtmann, L., N. Franck, H. Lerat, J. Le Seyec, D. Gilot, I. Cannie, P. Gripon, U. Hibner, C. Guguen-Guillouzo.
2003
. The hepatitis C virus NS2 protein is an inhibitor of CIDE-B-induced apoptosis.
J. Biol. Chem.
278
:
18256
-18264.
46
Prikhod'ko, E. A., G. G. Prikhod'ko, R. M. Siegel, P. Thompson, M. E. Major, J. I. Cohen.
2004
. The NS3 protein of hepatitis C virus induces caspase-8-mediated apoptosis independent of its protease or helicase activities.
Virology
329
:
53
-67.
47
Nomura-Takigawa, Y., M. Nagano-Fujii, L. Deng, S. Kitazawa, S. Ishido, K. Sada, H. Hotta.
2006
. Non-structural protein 4A of Hepatitis C virus accumulates on mitochondria and renders the cells prone to undergoing mitochondria-mediated apoptosis.
J. Gen. Virol.
87
:
1935
-1945.
48
Nanda, S. K., D. Herion, T. J. Liang.
2006
. The SH3 binding motif of HCV (corrected) NS5A protein interacts with Bin1 and is important for apoptosis and infectivity.
Gastroenterology
130
:
794
-809.
49
Meylan, E., J. Curran, K. Hofmann, D. Moradpour, M. Binder, R. Bartenschlager, J. Tschopp.
2005
. Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus.
Nature
437
:
1167
-1172.
50
Wang, J., W. Tong, X. Zhang, L. Chen, Z. Yi, T. Pan, Y. Hu, L. Xiang, Z. Yuan.
2006
. Hepatitis C virus non-structural protein NS5A interacts with FKBP38 and inhibits apoptosis in Huh7 hepatoma cells.
FEBS Lett.
580
:
4392
-4400.
51
Chung, Y. L., M. L. Sheu, S. H. Yen.
2003
. Hepatitis C virus NS5A as a potential viral Bcl-2 homologue interacts with Bax and inhibits apoptosis in hepatocellular carcinoma.
Int. J. Cancer
107
:
65
-73.
52
Tardif, K. D., G. Waris, A. Siddiqui.
2005
. Hepatitis C virus, ER stress, and oxidative stress.
Trends Microbiol.
13
:
159
-163.
53
MacFarlane, M..
2003
. TRAIL-induced signalling and apoptosis.
Toxicol. Lett.
139
:
89
-97.
54
Wagner, K. W., E. A. Punnoose, T. Januario, D. A. Lawrence, R. M. Pitti, K. Lancaster, D. Lee, M. von Goetz, S. F. Yee, K. Totpal, et al
2007
. Death-receptor O-glycosylation controls tumor-cell sensitivity to the proapoptotic ligand Apo2L/TRAIL.
Nat. Med.
13
:
1070
-1077.
55
Everett, H., G. McFadden.
1999
. Apoptosis: an innate immune response to virus infection.
Trends Microbiol.
7
:
160
-165.
56
Dunn, C., M. Brunetto, G. Reynolds, T. Christophides, P. T. Kennedy, P. Lampertico, A. Das, A. R. Lopes, P. Borrow, K. Williams, et al
2007
. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
J. Exp. Med.
204
:
667
-680.
57
Liang, X., Y. Liu, Q. Zhang, L. Gao, L. Han, C. Ma, L. Zhang, Y. H. Chen, W. Sun.
2007
. Hepatitis B virus sensitizes hepatocytes to TRAIL-induced apoptosis through Bax.
J. Immunol.
178
:
503
-510.
58
Zender, L., S. Hutker, B. Mundt, M. Waltemathe, C. Klein, C. Trautwein, N. P. Malek, M. P. Manns, F. Kuhnel, S. Kubicka.
2005
. NFκB-mediated upregulation of bcl-xl restrains TRAIL-mediated apoptosis in murine viral hepatitis.
Hepatology
41
:
280
-288.
59
Sato, K., S. Hida, H. Takayanagi, T. Yokochi, N. Kayagaki, K. Takeda, H. Yagita, K. Okumura, N. Tanaka, T. Taniguchi, K. Ogasawara.
2001
. Antiviral response by natural killer cells through TRAIL gene induction by IFN-α/β.
Eur. J. Immunol.
31
:
3138
-3146.
60
Pelli, N., F. Torre, A. Delfino, M. Basso, A. Picciotto.
2006
. Soluble tumor necrosis factor-related ligand (sTRAIL) levels and kinetics during antiviral treatment in chronic hepatitis C.
J. Interferon Cytokine Res.
26
:
119
-123.