Effector CD8 T cell recruitment into the skin in response to Ag challenge requires prior CXCL1/KC-directed neutrophil infiltration. Mechanisms inducing CXCL1 production and the dynamics of neutrophil-CD8 T cell interactions during elicitation of Ag-specific responses in the skin were investigated. CXCL1 and CXCL2/MIP-2 were produced within 3–6 h of Ag challenge at 10-fold higher levels in skin challenge sites of Ag-sensitized vs nonsensitized mice. In the challenge sites of sensitized mice this production decreased at 6–9 h postchallenge to near the levels observed in skin challenge sites of nonsensitized mice but rose to a second peak 12 h after challenge. The elevated early neutrophil chemoattractant production at 3–6 h after skin challenge of sensitized animals required both IFN-γ and IL-17, produced by distinct populations of Ag-primed CD8 T cells in response to Ag challenge. Although induced by the Ag-primed CD8 T cells, the early CXCL1 and CXCL2 production was accompanied by neutrophil but not CD8 T cell infiltration into the skin Ag challenge site. Infiltration of the CD8 T cells into the challenge site was not observed until 18–24 h after challenge. These results demonstrate an intricate series of early interactions between Ag-specific and innate immune components that regulate the sequential infiltration of neutrophils and then effector T cells into the skin to mediate an immune response.
A characteristic feature of inflammation is leukocyte infiltration into the tissue site. Neutrophils are typically the first leukocytes recruited to infiltrate tissue inflammation in response to the production of chemoattractants binding CXCR1 and CXCR2 that are constitutively expressed on circulating neutrophils (1, 2, 3, 4, 5, 6). In addition to directing recruitment, neutrophil chemoattractants such as IL-8 and CXCL1/KC activate neutrophils to release granules containing many chemokines, cytokines, and extracellular matrix degrading enzymes (3). The release of these mediators amplifies inflammation by recruiting other leukocyte populations into the tissue site and inflammation is often attenuated by antagonism of the initial neutrophil infiltration. In contrast to the constitutive expression of chemokine receptors by neutrophils, T cells are directed to peripheral tissues through the induced expression of specific integrins and chemokine receptors that complement the ligands expressed in that tissue. Human and mouse CD4 T memory cells trafficking to the skin express CCR4 and are directed to the skin through endothelial cell production of CCL17/TARC (7, 8). The requirements for Ag-primed CD8 T cell trafficking into the skin during elicitation of cutaneous immune responses are not as clearly defined as for CD4 T cells.
Contact hypersensitivity (CHS)3 is a T cell-mediated inflammatory response in the skin to cutaneous sensitization and subsequent challenge with a hapten. Hapten application to the skin triggers Ag acquisition by epidermal and dermal dendritic cells, including Langerhans cells, that migrate to the skin draining lymph nodes and prime hapten-specific T cell populations (9, 10, 11, 12). Following subsequent skin contact with the hapten, the primed T cells infiltrate the challenge site and are activated to express effector functions, including IFN-γ and TNF-α production, that mediate the characteristic tissue edema/spongiosis of the CHS response that peaks 18–48 h after challenge. Hapten-specific CD8 T cells are the primary effector cells of CHS responses to many haptens, including 2,4-dinitro-1-fluorobenzene (DNFB), oxazolone, and urushiol, the reactive hapten in poison ivy (13). CHS responses to these haptens are absent or substantially decreased in mice treated with CD8 T cell-depleting Abs and in mice with targeted gene deletions that result in the absence of mature CD8 T cell development (14, 15, 16). Consistent with this effector function, sensitization with DNFB and other haptens results in the activation and expansion of hapten-specific CD8 T cells producing IFN-γ, whereas hapten-primed CD4 T cells produce IL-4, IL-5, and IL-10 (16, 17, 18).
The factors directing Ag-primed CD8 T cells into challenged skin to mediate an immune response remain poorly understood. Previous studies indicated the inhibition of CHS responses when hapten-sensitized mice were treated with CXCL1-specific Abs at the time of hapten challenge (19). CXCL1 does not directly recruit hapten-primed CD8 T cells into the challenge site but recruits neutrophils that, in turn, induce effector CD8 T cell infiltration into the site to mediate the response. Furthermore, the amount of CXCL1 produced and the intensity of neutrophil infiltration into the skin following hapten challenge directly influences the number of effector T cells infiltrating the challenge site and the magnitude of the immune response elicited (20). It has been presumed that hapten application mediates an early inflammatory response that includes CXCL1 production, although the specific factors inducing this CXCL1 as well as the site of production during elicitation of CHS remain unknown. In the current study, we investigated these aspects of the initiation of the response as well as the relationship between this chemokine production and subsequent neutrophil and CD8 T cell infiltration into the hapten challenge site during CHS responses. The results indicate that hapten challenge is rapidly followed by stimulation of distinct populations of primed effector CD8 T cell populations to produce IFN-γ and IL-17. Both of these cytokines are required to induce the chemoattractants directing neutrophil infiltration into the challenge site. These results indicate that the early Ag-specific CD8 T cell production of IFN-γ and IL-17 triggers the innate immune response that subsequently directs the effector CD8 T cells into the skin to elicit the response.
Materials and Methods
BALB/c (H-2d) and C57BL/6 (H-2b) mice were obtained through the National Cancer Institute (Frederick, MD), CD4−/−, CD8−/−, and RAG-1−/− mice on the C57BL/6 background and IFN-γ−/− mice on the BALB/c background from The Jackson Laboratory, and IL-17−/− mice on the BALB/c background from Dr. Y. Iwakura, (University of Tokyo, Tokyo, Japan). Female mice, 8–10 wk of age, were used throughout these studies. All studies were approved by the Institutional Animal Care and Use Committee of the Cleveland Clinic.
Hapten sensitization and elicitation of CHS
Mice were sensitized to DNFB or oxazolone by painting the shaved abdomen with 25 μl of 0.25% DNFB (Sigma-Aldrich) or 25 μl of 1% oxazolone (Sigma-Aldrich) and 10 μl to each paw on days 0 and +1 (16). On day +5, hapten-sensitized and control nonsensitized mice were challenged on each side of each ear with 10 μl of DNFB or oxazolone. Ear thickness was measured using an engineer’s micrometer (Mitutoyo America) and expressed in units of 10−4 inches. The ear swelling response is given as the mean increase of each group (n = 4) of individual animals ± SEM.
For immunohistochemistry and flow cytometry the following Abs were used: anti-CD3, anti-CD8, and anti-CD45 mAb (BD Pharmingen), rat anti-mouse Gr-1 mAb (eBioscience). Anti-mouse IL-17 mAb was purchased from Southern Biotechnology Associates. Purified mAb YTS 191.1.2 and GK1.5 (anti-mouse CD4) and YTS 169 and TIB-150 (anti-mouse CD8) for in vivo treatment were purchased from BioXCell. RB6-8C5 (anti-mouse Gr-1 mAb), XMG1.2 (anti-mouse IFN-γ mAb), and 2C11 (anti-mouse CD3 mAb) were purified from culture supernatants by protein G chromatography.
Mice were depleted of CD4+ T cells by injecting 100 μg each of YTS 191 and GK1.5, i.p. on three consecutive days before hapten sensitization (16, 17, 18). Mice were injected with 100 μg each of YTS 169 and TIB-150 to deplete CD8+ T cells. Both CD4 and CD8 T cells were depleted by injecting mice with 200 μg of anti-CD3 mAb 2C11 on three consecutive days before hapten sensitization. Mice were injected with 150 μg of RB6-8C5 i.p. on days +4 and +5 following hapten sensitization to deplete neutrophils (19, 20). In each experiment, treated sentinel mice were used to evaluate the efficiency of CD4+ or CD8+ T cell depletion by Ab staining and flow cytometry analysis of spleen and lymph node cells (LNC) and was always >95% when compared with cells from control, rat IgG-treated mice.
Quantitation of CXCL1 and CXCL2 production by immunoassay
Production of CXCL1 and CXCL2 in skin following hapten application was determined using immunoassay kits (R&D Systems). Hapten-challenged skin was removed and homogenized in 500 μl of PBS with 0.01 M EDTA and in a proteinase inhibitor cocktail (10 μg/ml PMSF, 2 μg/ml aprotinin, 2 μg/ml leupeptin, 100 μg/ml Pefabloc SC, 100 μg/ml chymostatin). Then 1 ml of 1.5% Triton X-100 in PBS was added with shaking at 4°C for 30 min. After pelleting cell debris, the supernatants were collected and the total protein concentration quantified using a Coomassie Plus Protein Assay Reagent kit (Pierce). For each experiment, all samples were diluted to an equivalent total protein concentration.
T cell transfer
BALB/c mice were depleted of CD4 or CD8 T cells before sensitization to DNFB or oxazolone. On day + 4 following sensitization, 4 × 106 LNC aliquots were transferred i.v. to naive recipients that had been challenged on the shaved abdomen with 25 μl of 0.2% DNFB 1 h before cell transfer. The challenged skin was excised 6 h after challenge and CXCL1 production determined.
Analysis of tissue-infiltrating cells
After 6 or 18 h, the challenged skin of sensitized and nonsensitized, mice was excised and incubated in 0.5% dispase (Invitrogen) for 18 h at 4°C. The next day, the epidermis was separated from the dermis and incubated in 0.5% trypsin (Sigma-Aldrich) for 60 min at 37°C, 5% CO2. The epidermis and dermis were each pressed through dialysis tubing and the cells were incubated in 0.2% DNase (Roche) for 10 min at room temperature and 1 × 106 cell aliquots were washed in staining buffer (Dulbecco’s PBS with 2% FCS/0.2% NaN3) and incubated in 100 μl of Fc block (BD Pharmingen) diluted 1/100 in the staining buffer for 20 min on ice. To examine leukocytes infiltrating the skin challenge site, cell aliquots were washed and stained with fluorochrome-labeled anti-mouse mAb to CD45, CD3, and Gr-1. After 30 min, the cells were washed, resuspended in staining buffer and analyzed by two-color flow cytometry using a FACScan and CellQuest software (BD Biosciences). The cells were gated to exclude nonviable cells and sample data were collected on 20,000 cells.
To detect leukocyte infiltration into challenged skin, the tissue was excised, fixed in 10% formalin, and embedded in paraffin and 8-μm sections were stained with H&E. To detect chemokine protein, the excised skin was fixed with 10% formalin, and 8-μm paraffin-embedded sections were mounted onto slides. The slides were deparaffinized, rehydrated, and boiled in an Ag-retrieval solution (BioGenex Laboratories). Slides were stained with CXCL1-specific (5 μg/ml) goat antiserum diluted in PBS/1% BSA solution overnight at 4°C. Control slides were incubated with normal goat serum (Vector Laboratories). Primary Ab binding was detected using biotinylated rabbit anti-goat IgG followed by streptavidin HRP and developed using the substrate chromagen 3,3′-diaminobenzidine. For staining to detect neutrophils or CD8 T cells, excised skin was embedded in OCT compound (Sakura Finetek) and frozen in liquid nitrogen, and 8-μm sections were mounted onto slides. The slides were dried, fixed in acetone, air-dried, and rehydrated with PBS before immersion in 0.03% H2O2 for 10 min. Slides were stained with CD8 (10 μg/ml) or RB6-8C5 Ab (10 μg/ml) diluted in PBS/1% BSA and control slides were incubated with rat IgG. Primary Ab was detected using biotinylated rabbit anti-rat IgG and developed as described. After a final wash in dH2O, slides were counterstained with hematoxylin, rinsed, and dehydrated for mounting. Images were captured using ImagePro Plus 5.0 (Media Cybernetics).
Analysis of gene expression by quantitative RT-PCR
Whole cell RNA was obtained by dissolving homogenates from challenged skin in TRIzol reagent (Invitrogen Life Technologies) with subsequent chloroform extraction. cDNA was synthesized from 2 μg of RNA using the TaqMan Reverse Transcription Reagent kit (Applied Biosystems). PCR was performed using custom primers and FAM dye-labeled probes (Applied Biosystems) for mouse TNF-α, IFN-γ, IL-17, CXCL1, CXCL2, IL-10, IL-21, and Mrpl 32 (gene assay ID nos. Mm00443258_m1, Mm00801778_m1, Mm00439619_m1, Mm00433859_m1, Mm00436450_m1, Mm00439616_m1, Mm00517640_m1, and Mm00777741_sH, respectively). The comparative threshold cyclic CT method for relative quantitation of cytokine gene expression was used where log measurements for each sample are made during amplification and the expression level of the Mrpl 32 housekeeping gene is subtracted from the expression level for each test cytokine gene. For each test cytokine, the expression level of a single RNA sample prepared from the challenged skin of sensitized, untreated wild-type mice was used as the calibrator and was arbitrarily set at 1.0 and the expression levels of all other samples were then normalized to the calibrator. Duplicate runs of each individual RNA sample prepared from a single mouse of 3–4 mice per group were tested and the data from 3–4 RNA samples for each group are expressed as mean test cytokine expression level ± SEM.
Enumeration of hapten-specific T cells producing IFN-γ or IL-17
ELISPOT assays were performed as described (21). Briefly, ELISPOT plates (Unifilter 350; Polyfiltronics) were coated with 4 μg/ml IFN-γ or 2 μg/ml IL-17 mAb and blocked with 1% BSA in PBS. LNC from sensitized or nonsensitized wild-type BALB/c, IFN-γ−/−, or IL-17−/− mice were prepared on day +5 as responder cells. Spleen cells from naive mice were incubated with 50 μg/ml mitomycin C for 30 min at 37°C, washed, and labeled with DNBS (2, 4-dinitrobenzene sulfonic acid) for 1 h for use as stimulator cells. Stimulator cells were plated at 5 × 105 cells/well with 2.5 × 105 responder cells/well in serum-free HL-1 medium (BioWhittaker) supplemented with 1 mM l-glutamine and 1 mM antibiotic. After 24 h, cells were removed by extensive washing with PBS/0.05% Tween 20. Biotinylated anti-IFN-γ mAb (2 μg/ml) or anti-IL-17 mAb (1 μg/ml) was added and the plate incubated overnight at 4°C. The plate was washed with PBS/0.05% Tween 20 and conjugated streptavidin-alkaline phosphatase added to each well. After 2 h at room temperature, the plates were washed with PBS/0.05% Tween 20 and NBT/5-bromo-4-chloro-30-indolyl substrate (Bio-Rad) added. The resulting spots were counted with an ImmunoSpot Series I Analyzer (Cellular Technology).
Statistical analysis to assess differences between experimental groups was performed using Student’s t test. Differences were considered significant for values of p < 0.05.
Bimodal production of CXCL1/KC and CXCL2/MIP-2 during elicitation of CHS
The temporal production of the neutrophil chemoattractants CXCL1 and CXCL2 in skin challenge sites during elicitation of CHS was investigated. Groups of DNFB-sensitized and naive/nonsensitized mice were challenged with DNFB and at various times postchallenge tissue homogenates of the skin challenge site were prepared and the production of the neutrophil chemoattractant proteins was tested. In naive mice DNFB application induced low levels of CXCL1 and CXCL2, first evident 6 h later and maintained at low levels before falling to background levels by 12–24 h after application (Fig. 1). In contrast, hapten challenge of sensitized mice induced bimodal production of CXCL1 and CXCL2 with production evident as early as 3 h postchallenge and at levels 6- to 10-fold higher than observed in challenged skin of naive mice at any time. CXCL1 production reached peak at 6 h postchallenge and then fell near to levels observed in naive mice and then increased again to peak levels at 12 h after challenge followed by a second decline. CXCL2 production reached peak 3 h after challenge of sensitized mice, decreased and then reached a second peak at 12 h postchallenge.
CD8 T cells mediate the early CXCL1 and CXCL2 production in sensitized mice
The high levels of CXCL1 and CXCL2 produced shortly after challenge of hapten-sensitized mice suggested the ability to quickly recognize and react to the hapten. In sensitized mice treated with both anti-CD4 plus anti-CD8 mAb to deplete T cells before DNFB sensitization, CXCL1 levels induced by Ag challenge were virtually identical with those of nonsensitized naive mice following challenge (results not shown). Similarly, DNFB challenge of sensitized B6.RAG-1−/− mice or sensitized wild-type C57BL/6 mice depleted of T cells by treatment with anti-CD3 mAb induced markedly decreased levels of CXCL1 and CXCL2 for 6 h after challenge when compared with the levels induced by challenge of sensitized wild-type mice (Fig. 2, A and B). The role of CD4 vs CD8 T cells in this early neutrophil chemoattractant production was then tested. First, groups of mice were treated with control rat IgG or with specific mAb to deplete either CD4 or CD8 T cells before sensitization with DNFB. Skin was excised either 3 or 6 h after challenge to test CXCL1 and CXCL2 production, respectively. Following skin challenge of sensitized mice depleted of CD8 but not CD4 T cells, CXCL1 production was decreased to naive levels (Fig. 2,C). CXCL2 production was equivalent in sensitized animals treated with control rat IgG or CD4 T cell depleting mAb but was significantly reduced in sensitized animals depleted of CD8 T cells (Fig. 2,D). Similarly, early CXCL1 and CXCL2 production was not detected after challenge of sensitized CD8-deficient mice but was slightly enhanced in sensitized CD4-deficient mice when compared with levels in sensitized wild-type animals (Fig. 2, E and F).
Ag-specificity of early neutrophil chemoattractant production following challenge to elicit CHS
Because the early CXCL1 and CXCL2 production was dependent on CD8 T cells from hapten-sensitized mice, the Ag-specificity of this production was tested. First, mice were sensitized with DNFB or with oxazolone and on day +5 the animals were challenged with either the sensitizing hapten or with the irrelevant hapten. Challenged skin was excised from all mice 6 h after challenge, and the levels of CXCL1 production were tested in tissue homogenates. Sensitization and challenge with DNFB induced high levels of CXCL1 production (136.4 ± 10.1 pg/mg lysate protein) and higher levels (272.9 ± 11.5 pg/mg lysate protein) were induced by sensitization and challenge with oxazolone (Fig. 3). Low levels of CXCL1 were induced in naive mice when either DNFB (9.6 ± 0.3 pg/mg lysate protein) or oxazolone (65.2 ± 13.6 pg/mg lysate protein) was applied to the skin. Oxazolone challenge of DNFB-sensitized mice induced CXCL1 levels similar to oxazolone application to naive animals and DNFB challenge of oxazolone-sensitized mice induced levels similar to DNFB application to naive animals.
As a second approach, separated populations of CD4 and CD8 T cells from naive, DNFB-sensitized, or oxazolone-sensitized donors were transferred to naive mice and the production of CXCL1 was determined 6 h after recipient skin challenge with DNFB (Fig. 4). Transfer of DNFB-immune CD8 but not CD4 T cells induced high levels of CXCL1 production in response to DNFB challenge of the recipient skin. Low to absent CXCL1 production was observed following DNFB challenge in recipients of CD8 T cells from either oxazolone-immune or naive donors. These results indicate that primed CD8 T cell induction of early CXLC1 production in response to hapten challenge to elicit CHS is hapten-specific.
Neutrophils but not CD8 T cells infiltrate challenge sites 6 h after challenge to elicit CHS
The hapten-primed CD8 T cell mediated neutrophil chemoattractant production within 3–6 h after challenge of sensitized mice, suggesting infiltration of both CD8 T cells and neutrophils into the challenged skin at that time. Cellular infiltration into the DNFB challenge site of DNFB-sensitized vs nonsensitized mice was investigated 6 and 18 h after DNFB challenge by staining tissue sections with H&E (Figs. 5, A–C and 6, A and B) or with Abs to specifically detect infiltrating neutrophils and CD8 T cells (Figs. 5, D and F, and 6, C and D). DNFB challenge of naive mice induced little cellular infiltration (Fig. 5,A), whereas challenge of sensitized mice induced infiltration with polymorphonuclear leukocytes within 6 h that stained positively for Abs that detect neutrophils (Fig. 5, B and D). Neutrophil infiltration into the skin 6 h after hapten challenge was dependent on CD8 T cells as this infiltration was absent following challenge of mice depleted of CD8 T cells during DNFB sensitization (Fig. 5, C and F). In contrast to neutrophils, infiltrating CD8 T cells were not observed 6 h after challenge of sensitized animals (Fig. 5,E) but both neutrophils and CD8 T cells were observed 18 h after challenge of sensitized mice (Fig. 6, C and D).
These data were extended using a flow cytometry approach. Skin was excised 6 and 18 h after challenge and digested to prepare single cell suspensions and cell aliquots were stained with anti-CD45 mAb plus Abs to detect the presence of neutrophils and T cells in the challenge site. Consistent with the histological analyses, few Gr-1+ cells infiltrated the skin of naive mice after application of hapten but this infiltration was clearly evident 6 and 18 h following challenge of sensitized mice and was absent when the animals were depleted of CD8 T cells (Figs. 5,G and 6 E). The Gr-1+ cells were not positive for F4/80, indicating that they were not infiltrating Gr-1+ monocytes or macrophages (data not shown). The skin digestion protocol does not allow for CD8 staining of the cells but CD3 T cell infiltration into the site was evident 18 but not 6 h after challenge.
CD8 T cells producing IFN-γ and IL-17 stimulate early production of CXCL1 leading to elicitation of the CHS response
Recent studies have indicated a need for hapten-primed T cells producing IL-17 to elicit CHS responses in hapten-sensitized mice (22, 23). Previous studies from this laboratory have implicated a role for IFN-γ in the elicitation of CHS (16, 17, 18). To investigate these previous findings in greater detail, we first tested the elicitation of CHS responses following DNFB sensitization and challenge of wild-type vs IFN-γ−/− and IL-17−/− mice. The CHS responses to hapten challenge of sensitized IFN-γ−/− and IL-17−/− were markedly decreased when compared with responses elicited in sensitized wild-type mice (Fig. 7,A). Similarly, treatment of DNFB-sensitized wild-type mice with either anti-IFN-γ or anti-IL-17 mAb at the time of hapten challenge significantly attenuated the CHS responses elicited (Fig. 7 B). These results indicated a requirement for both IFN-γ and IL-17 in the elicitation of CHS responses.
The presence of hapten-specific CD8 T cells producing IFN-γ and IL-17 in the skin draining lymph nodes of sensitized mice was tested by ELISPOT assay. In sensitized wild-type mice, high numbers of hapten-specific CD8 T cells producing IFN-γ were present as well as lower numbers of hapten-specific CD8 T cells producing IL-17 (Fig. 8,A). In sensitized IL-17−/− mice, a high number of IFN-γ and not IL-17 producing CD8 T cells was present. Alternatively, in sensitized IFN-γ−/− mice, a high number of IL-17- but not IFN-γ-producing CD8 T cells were detected. T cells from the skin draining lymph nodes of DNFB-sensitized mice were stimulated in vitro, and CD4 and CD8 T cell populations were stained for IFN-γ and IL-17 to detect the cytokines by flow cytometry (Fig. 8 B). These analyses indicated that separate populations of hapten-primed CD8 T cells produce the IFN-γ and IL-17.
Because primed CD8 T cells are activated within 3–6 h in the hapten challenge site to induce CXCL1 and CXCL2 production, the expression of IFN-γ and IL-17 in the skin challenge site at early time points during elicitation of CHS was tested. Groups of mice were treated with depleting anti-CD8 or anti-CD4 mAb or with control rat IgG, and were sensitized with DNFB. On day +5 the sensitized and a group of naive mice were challenged on the skin with DNFB and 6 h later the challenged skin was retrieved to prepare whole cell RNA. Both IFN-γ and IL-17 mRNA were expressed in the challenge site from sensitized but not naive mice (Fig. 9). Depletion of CD8 T cells before hapten sensitization ablated the appearance of IFN-γ and IL-17 mRNA expression in response to challenge, whereas depletion of CD4 T cells increased the mRNA expression of both cytokines.
Next, the role of IL-17 and IFN-γ in inducing early CXCL1 and CXCL2 during elicitation of CHS was tested. Treatment of sensitized mice 30 min after hapten challenge with anti-IFN-γ or anti-IL-17 mAb reduced CXCL1 produced in the skin 6 h later to the levels observed in challenged naive mice (Fig. 10,A). Similarly, hapten challenge of either DNFB sensitized IFN-γ−/− or IL-17−/− mice resulted in naive levels of CXCL1 and CXCL2 at 6 h postchallenge (Fig. 10, B and C).
The recruitment and activation of hapten-primed CD8 T cells producing only IFN-γ or IL-17 within the challenge site was then investigated. DNFB-sensitized wild-type, IFN-γ−/−, and IL-17−/− mice were challenged with DNFB and 6 h later the skin was removed and prepared RNA was analyzed for expression of the cytokine genes as an indication of the activation of the CD8 T cells in the site (Fig. 11,A). Again, the absence of CD8 T cells producing either IFN-γ or IL-17 resulted in naive levels of CXCL1 expression in response to challenge. Although the increased number of IL-17-producing CD8 T cells were induced by sensitization of IFN-γ−/− vs wild-type mice (Fig. 8,A), expression of IL-17 mRNA during challenge of the sensitized IFN-γ−/− mice was reduced to the levels observed following challenge of the naive mice. Similarly, challenge of sensitized IL-17−/− mice induced reduced levels of IFN-γ mRNA when compared with the levels induced following challenge of sensitized wild-type mice. High levels of mRNA expression of TNF-α, IL-10, and IL-21 were also present In the skin challenge site of hapten-sensitized mice 6 h after challenge and these levels were decreased to those observed following challenge of naive mice in wild-type mice depleted of CD8 T cells as well as following challenge of sensitized IFN-γ−/− and IL-17−/− mice (Fig. 11 A).
Finally, the ability of transferred CD8 T cells from sensitized wild-type, IFN-γ−/− and IL-17−/− mice were compared for the ability to induce CXCL1 production 6 h after hapten skin challenge of naive mice. As previously observed (Fig. 4), transfer of immune CD8 T cells from sensitized wild-type to naive mice induced high levels of CXCL1 production in the challenged skin (Fig. 11 B). Transfer of CD8 T cells from sensitized IFN-γ−/− or IL-17−/− mice induced low levels of the chemokine that were similar to those observed in the skin of naive mice challenged with the hapten. However, transfer of CD8 T cells from sensitized IFN-γ−/− plus CD8 T cells from sensitized IL-17−/− induced CXCL1 levels that were similar to those observed following challenge of sensitized wild-type mice.
The trafficking of Ag-primed T cells through the vascular endothelium and into parenchymal tissue during elicitation of immune responses requires a complex series of cellular and molecular interactions. The expression and role of tissue-specific adhesion molecules, chemokines, and their respective receptors have been documented for CD4 T cells infiltrating the skin and intestines (7, 24, 25). For elicitation of many immune responses, prior neutrophil infiltration and activation is required for T cell infiltration into peripheral tissue sites. Depletion of neutrophils or inhibition of neutrophil trafficking has been shown to inhibit or attenuate allograft rejection and delayed-type hypersensitivity responses to erythrocyte, tuberculin, and viral Ags (2, 26, 27, 28, 29). CXCL1-mediated neutrophil infiltration is required for effector CD8 T cell trafficking into hapten challenge sites to mediate CHS and the levels of CXCL1 produced correlate with the infiltration of both neutrophils and hapten-primed CD8 T cells into the challenge site (19, 20). The mechanisms inducing CXCL1 production and neutrophil infiltration into inflammatory sites to initiate T cell recruitment and the elicitation of immune responses in the skin remain unclear and were the focus of the current studies.
The results indicate an intricate series of cellular interactions where production of the chemokines directing neutrophil infiltration into hapten challenge sites during the early stages of CHS is induced by hapten-primed CD8 T cells. The first step in the initiation of this response is the presentation of the hapten in the challenge site to hapten-specific CD8 T cells and their activation to produce IL-17 and IFN-γ. These cytokines stimulate the CXCL1 and CXCL2 production that, in turn, direct infiltration of neutrophils into the parenchymal tissue of the challenge site. Both immunohistology and flow cytometry analyses of infiltrating cell populations clearly indicate that hapten-primed CD8 T cell induction of CXCL1 within hours after challenge is accompanied by neutrophil but not CD8 T cell infiltration into the site. This initial neutrophil infiltration is followed several hours later by entry of hapten-primed effector CD8 T cells into the site to mediate the resulting response.
The consequence of neutrophil infiltration and activation in the challenge site at 6 h postchallenge is a low level edematous response (D. Kish, unpublished observations and data not shown). An early hapten-specific tissue-swelling component of CHS responses following hapten sensitization and challenge was originally reported by the Askenase laboratory (30). They have also reported that the induction of this early swelling response is dependent on C5a but, in contrast to the results presented in this report, is independent of Ag-specific T cells (31, 32). This discrepancy is likely due to interpretation of the observed absence of hapten-primed T cell infiltration into the parenchymal tissue. The low magnitude of the swelling response 6 h after challenge reflects this absence and it is not until T cells infiltrate the tissue at later times (12–18 h) postchallenge that peak levels of edema/swelling are elicited in the challenge site. C5a has chemotactic and activating effects on many different cells including endothelial cells, T cells and neutrophils and the role of C5a as well as the cells expressing C5aR during the generation of this early swelling response in hapten-sensitized animals remain unclear.
Two major questions that arises from these studies is what cells present the hapten to the effector CD8 T cell populations and what cells produce the neutrophil chemoattractants in response to the IL-17 and IFN-γ produced by the effector CD8 T cells? It is worth noting that TCR-mediated or mitogenic stimulation of purified effector CD8 T cells does not result in CXCL1 production suggesting that it is not the effector T cells producing CXCL1 in response to hapten skin challenge. Vascular endothelial cells are the first cells that circulating Ag-primed T cells encounter at an inflammatory site before infiltration into peripheral tissues. Although direct perfusion of hapten-protein complexes through the blood into the spleen and through the afferent lymph to the nodes following cutaneous hapten application has been previously documented (33), the presentation of hapten by cutaneous vascular endothelial cells has not. Preliminary histological studies suggest that hapten application to the skin results in endothelial cell acquisition of hapten and activation of the CD8 T cells to produce IL-17 and IFN-γ that in turn stimulates the endothelial cells to produce CXCL1 and CXCL2 (D. Kish, unpublished observations). During many inflammatory responses various components of tissue injury directly induce neutrophil chemoattractant production. In ischemic tissues, including solid organ grafts, reperfusion rapidly induces endothelial cell production of IL-8, CXCL1, CXCL2, and other neutrophil chemoattractants and is followed by neutrophil infiltration independently of T cells or NK cells (34, 35, 36, 37).
Previous studies have documented decreased CHS responses to DNFB and to TNCB (2,4,6-trinitorochlorobenzene) in IL-17−/− mice (23). More recently He et al. (22) demonstrated the priming of IL-17- and IFN-γ-producing CD8 T cells in response to sensitization with DNFB and that treatment of sensitized mice with anti-IL-17 mAb but not with anti-IFN-γ mAb during challenge decreased the CHS response. These results suggested that the IL-17-producing but not the IFN-γ-producing CD8 T cells were the effector T cells required to elicit the CHS response to hapten challenge. These results are in contrast to the current studies using both mAb neutralization and CD8 T cells from sensitized IL-17−/− and IFN-γ−/− mice to document the CD8 T cell-mediated production of both IL-17 and IFN-γ in the hapten challenge site as well as the requirement for both cytokines to induce the neutrophil chemoattractants required to activate the innate immune component of the CHS response. Most studies investigating the role of IL-17 in inflammatory responses, including autoimmune disease, have focused on CD4+ T cells (i.e., TH17) producing the cytokine (38, 39). Immunofluorescent analysis of T cells in active brain lesions of multiple sclerosis patients revealed both CD4+ and CD8+ T cells costaining with anti-IL-17 Abs (40). Whether the hapten-specific CD8 T cell populations producing IL-17 and IFN-γ to induce CXCL1 and CXCL2 early after challenge are a distinct population from the CD8 T cells entering the challenge site at later time points postchallenge to mediate CHS remains unclear. However, transfer of primed CD8 T cells to naive mice reproduced both the early neutrophil chemoattractant production and the CHS response when the recipients were challenged with hapten.
The modulatory effects of IL-17 and IFN-γ during the initiation and course of immune responses are complex. IL-17 stimulates cultured epithelial and venous endothelial cells to produce IL-8 and CXCL1, and IL-17-induced human bronchial epithelial cell production of IL-8 is enhanced by IFN-γ (41, 42, 43). However, both in vitro and in vivo studies have indicated that IFN-γ negatively regulates TH17 cell development as well as IL-17 production by differentiated CD4 TH17 cells (43, 44, 45). Recent studies have indicated that peptide vaccine-induced CD4 TH17 cells arrive in infected lungs 3 days before CD4 TH1 cells and induce the chemokines mediating TH1 cell recruitment (46). Although hapten-specific CD8 T cells producing IL-17 are induced by sensitization of IFN-γ−/− mice, challenge did not result in detectable activation of these T cells in the challenge site as indicated by the low expression of CXCL1 or IL-17. Similarly, hapten-specific CD8 T cells producing IFN-γ are induced by sensitization of IL-17−/− mice but activation of these T cells at the site of challenge was not detectable by the expression of CXCL1 or IFN-γ. However, transfer of IFN-γ-producing CD8 T cells from sensitized IL-17−/− mice with IL-17-producing CD8 T cells from sensitized IFN-γ−/− mice restored early CXCL1 production to hapten challenge. These results not only indicate that both IFN-γ- and IL-17-producing CD8 T cells are required to stimulate CXCL1 production at the challenge site but that activation of each cytokine-producing CD8 T cell population promotes the recruitment or retention of the other at the site.
Although the hapten-primed CD8 T cells induce early CXCL1 and CXCL2 in the hapten challenge site they do not traverse the endothelial barrier into the tissue parenchyma without prior neutrophil infiltration. The neutrophil functions directing Ag-primed T cell infiltration into the site are yet not known. Depletion of neutrophils resulted in slightly increased levels of CXCL1 and CXCL2 production at 6 h (results not shown), which are likely due to the absence of neutrophil-mediated digestion of the chemokines during transendothelial cell migration (47). Cytokine activation also induces neutrophils to produce T cell chemoattractants such as CXCL9/Mig and CXCL10/IP-10 during transendothelial migration and peripheral tissue infiltration and these chemokines are candidate-recruiting factors for directing the subsequent infiltration of Ag-primed T cells into the tissue (48, 49, 50, 51). Neutrophil-dependent leukocyte infiltration into the murine liver during CMV infection is associated with neutrophil expression of specific matrix metalloproteinases (52), suggesting that digestion and possibly structural alteration of extracellular matrix may be required for T cell infiltration into peripheral tissues during certain immune responses.
The results of these experiments demonstrate a dynamic series of interactions between the innate and adaptive immune responses to achieve Ag-primed T cell infiltration into a tissue site to elicit an immune response. There has been increasing interest in dissecting innate immune mechanisms regulating adaptive responses, particularly in the modulation of APC function. The current results indicate the requirement for Ag-specific responses to provoke the innate immunity required for T cell infiltration into the tissue site to elicit the immune response. The neutrophil dependency of T cell infiltration into peripheral tissue sites of other immune responses may also involve similar initial T cell control of neutrophil activity. The requirement for such interactions to elicit responses in the skin offers several new points at which regulation of both the adaptive and innate responses can be manipulated in a positive or negative manner to influence the magnitude and course of these immune responses.
We thank the staff of the Cleveland Clinic Biological Resources Unit for excellent care of the animals used in this study and Dr. Booki Min and Dr. Jeong-Su Do for help with the intracellular cytokine staining and analysis.
The authors have no financial conflict of interest.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This work was supported by Grants R01 AI45888 from the National Institute of Allergy and Infectious Diseases.
Abbreviations used in this paper: CHS, contact hypersensitivity; DNFB, 2,4-dinitro-1-fluorobenzene; LNC, lymph node cell.