Yue, F. Y., C. Lo, A. Sakhdari, E. Y. Lee, C. M. Kovacs, E. Benko, J. Liu, H. Song, R. B. Jones, P. Sheth, D. Chege, R. Kaul, and M. A. Ostrowski. 2010. HIV-specific IL-21 producing CD4+ T cells are induced in acute and chronic progressive HIV infection and are associated with relative viral control. J. Immunol. 185: 498–506.

In Fig 2A, the statistical symbols should not be placed in the CMV Ag conditions, as the differences between groups are not statistically significant.

FIGURE 2.

Viral-specific IL-21–producing cells are detectable in HIV infection. In A, summary data of IL-21–producing CD4+T cells in response to HIV p55 Ag, CMV lysate, and SEB, and stained as described in Fig. 1. The x-axis represents clinical group. LTNP with VL <200 copies/ml. The y-axis represents % CD4+T cells producing IL-21. In B, PBMCs from a normal volunteer (neg) (OM611), an HIV-infected LTNP (OM25, plasma VL <50 copies/ml), two chronic progressors (C) (OM 2, VL = 5000 copies/ml; OM7, VL = 373,631 copies/ml), and two acute seroconverters (A) (OM5029, VL = 12,832 copies/ml; OM5018, VL = 29,000) were cultured in the presence of p55 Ag in plain medium and were assessed for IL-21 by ELISA at 7 d. A low VL during acute/chronic HIV infection was defined as <20,000 copies/ml. In C, comparisons between IL-21 responses to HIV p55 Ag were performed in the same HIV-infected individuals listed in (B) by ELISA and intracellular flow cytometry. In D, IL-21 mRNA is demonstrated from PBMCs (read from left to right) that were treated with either 1 μg/ml SEB, 5 μg/ml HIV p55, or medium control for 6 h, lysed, and RNA was extracted as described in the Materials and Methods. RNA samples from these various subjects and stimulation conditions were diluted to 10 ng/μl and quantitated by one-step Taqman RT-qPCR as described in the Materials and Methods. Relative quantitations of IL-21 and TBP (housekeeping gene) mRNA were assigned by comparison with a standard curve that was generated by serial dilutions of RNA from SEB stimulated PBMCs taken from an HIV-uninfected subject. All samples were analyzed in quadruplicate. Shown are mean ratios of IL-21/TBP relative quantitations expressed as arbitrary units. Error bars represent SE. PBMCs from the following subjects were examined: OM2 (chronic progressor, VL = 5000 copies/ml, C low), OM4 (LTNP, VL = 49 copies/ml, LTNP low), OM14 (chronic progressor, VL = 7000 copies/ml, C low), OM442 (acute seroconverter, VL = 26,388 copies/ml, A high), OM5037 (acute seroconverter, VL = 13,948 copies/ml, A low), OM 5037 (HIV-uninfected, neg). Subjects OM2, OM4, OM14, and OM5037 had HIV p55 IL-21–producing CD4+ T cells >0.03%, whereas OM442 and 5037 did not have detectable frequencies of >0.02%. In E, summary data of cytokine producing CD4+T cells in the HIV cohort in response to HIV p55 Ag. The x-axis shows specific cytokine response to HIV. IL-2 and IFN-γ represents cells producing both cytokines in response to Ag, which are subsets of IFN-γ and IL-2 responses. Comparisons with statistical significance are shown as ***p < 0.005; **p < 0.05.

FIGURE 2.

Viral-specific IL-21–producing cells are detectable in HIV infection. In A, summary data of IL-21–producing CD4+T cells in response to HIV p55 Ag, CMV lysate, and SEB, and stained as described in Fig. 1. The x-axis represents clinical group. LTNP with VL <200 copies/ml. The y-axis represents % CD4+T cells producing IL-21. In B, PBMCs from a normal volunteer (neg) (OM611), an HIV-infected LTNP (OM25, plasma VL <50 copies/ml), two chronic progressors (C) (OM 2, VL = 5000 copies/ml; OM7, VL = 373,631 copies/ml), and two acute seroconverters (A) (OM5029, VL = 12,832 copies/ml; OM5018, VL = 29,000) were cultured in the presence of p55 Ag in plain medium and were assessed for IL-21 by ELISA at 7 d. A low VL during acute/chronic HIV infection was defined as <20,000 copies/ml. In C, comparisons between IL-21 responses to HIV p55 Ag were performed in the same HIV-infected individuals listed in (B) by ELISA and intracellular flow cytometry. In D, IL-21 mRNA is demonstrated from PBMCs (read from left to right) that were treated with either 1 μg/ml SEB, 5 μg/ml HIV p55, or medium control for 6 h, lysed, and RNA was extracted as described in the Materials and Methods. RNA samples from these various subjects and stimulation conditions were diluted to 10 ng/μl and quantitated by one-step Taqman RT-qPCR as described in the Materials and Methods. Relative quantitations of IL-21 and TBP (housekeeping gene) mRNA were assigned by comparison with a standard curve that was generated by serial dilutions of RNA from SEB stimulated PBMCs taken from an HIV-uninfected subject. All samples were analyzed in quadruplicate. Shown are mean ratios of IL-21/TBP relative quantitations expressed as arbitrary units. Error bars represent SE. PBMCs from the following subjects were examined: OM2 (chronic progressor, VL = 5000 copies/ml, C low), OM4 (LTNP, VL = 49 copies/ml, LTNP low), OM14 (chronic progressor, VL = 7000 copies/ml, C low), OM442 (acute seroconverter, VL = 26,388 copies/ml, A high), OM5037 (acute seroconverter, VL = 13,948 copies/ml, A low), OM 5037 (HIV-uninfected, neg). Subjects OM2, OM4, OM14, and OM5037 had HIV p55 IL-21–producing CD4+ T cells >0.03%, whereas OM442 and 5037 did not have detectable frequencies of >0.02%. In E, summary data of cytokine producing CD4+T cells in the HIV cohort in response to HIV p55 Ag. The x-axis shows specific cytokine response to HIV. IL-2 and IFN-γ represents cells producing both cytokines in response to Ag, which are subsets of IFN-γ and IL-2 responses. Comparisons with statistical significance are shown as ***p < 0.005; **p < 0.05.

Close modal

In Fig 2E, the statistical symbols have been corrected from *** to ** for IFN-γ versus IL-2 in HIV/chronic and for IFN-γ versus IL-2 for HIV/LTNP. The symbols were removed from IFN-γ versus IL-2 for HIV/acute.

The corrected figure is shown on the following page. The published figure legend is correct but is shown here for reference.