Sharma, R., P. R. Sharma, Y.-C. Kim, N. Leitinger, J. K. Lee, S. M. Fu, and S.-T. Ju. 2011. IL-2–controlled expression of multiple T cell trafficking genes and Th2 cytokines in the regulatory T cell-deficient scurfy mice: implication to multiorgan inflammation and control of skin and lung inflammation. J. Immunol. 186: 1268–1278.
The third sentence of the Abstract should read “Sf mice lacking the Il2 gene (Sf.Il2–/–), despite being devoid of Treg, did not develop skin and lung inflammation, but the inflammation in liver remained.”
The second panel of Fig. 6B did not have a scale on the y-axis. The corrected figure is shown below. The figure legend was correct as published and is reproduced below for reference.
Intracellular cytokine profiles of CD4+ T cells. Cells from the LN of 3-wk-old male B6, Sf, and Sf.Il2–/– mice (n ≥ 4) were stimulated ex vivo with PMA and ionomycin in the presence of monensin for 4 h and then stained for intracellular cytokines as described in Materials and Methods. A, The percentage of CD4+ T cells expressing the indicated cytokines was determined by flow cytometry on gated CD4+ T cells. The data of a representative mouse sample are shown. B, Compilation of data from individual samples. Each dot represents an individual mouse sample. The mean is indicated by a bar. *p < 0.05.
Intracellular cytokine profiles of CD4+ T cells. Cells from the LN of 3-wk-old male B6, Sf, and Sf.Il2–/– mice (n ≥ 4) were stimulated ex vivo with PMA and ionomycin in the presence of monensin for 4 h and then stained for intracellular cytokines as described in Materials and Methods. A, The percentage of CD4+ T cells expressing the indicated cytokines was determined by flow cytometry on gated CD4+ T cells. The data of a representative mouse sample are shown. B, Compilation of data from individual samples. Each dot represents an individual mouse sample. The mean is indicated by a bar. *p < 0.05.
In Table I, in the left column under the section “Upregulated in Sf and Sf.Il2–/– versus B6” and in the footnote, the “↑” symbols should have been published as “*”. The corrected table is shown on the next page.
| Gene | Sf versus B6 | Sf.Il2−/− versus B6 | Sf versus Sf.Il2−/− | Cell Types | Ligand/Receptor |
|---|---|---|---|---|---|
| Upregulated in Sf versus Sf.Il2−/− | |||||
| Cysltr1 | 89 | 3 | 32 | Th2 | LTC4, LTD4, LTE4 |
| Ltb4r1 | 8 | 1 | 9 | Th2 | LTB4 |
| Il1rl1 | 12 | 1 | 14 | Th2 | IL-33 |
| Itgae | 9 | ↓ 2 | 18 | T cells | E-cadherin |
| Ccr1 | 10 | 1 | 8 | Inflammatory T cells | CCL3, 5, 7 |
| Ccr2 | 6 | 3 | 2 | Th1 | CCL2 |
| Ccr8 | 3 | 1 | 3 | T cells in asthma | CCL1 |
| Cxcr6 | 11 | 4 | 3 | Induced by IL-2 IL-15 | CXCL16 |
| Ccl1 | 24 | 6 | 4 | T cells in asthma | CCR8 |
| Ccl8 | 3 | 1.5 | 2 | Mast, Eos, Baso | CCR1, 2B, R5 |
| Cxcl2 | 4 | 1 | 4 | Monocytes, Mϕ | CXCR2 |
| Selp | 2 | 1 | 2 | Skin-homing T cells | P-selectin ligand |
| L1cam | 1 | ↓ 3 | 2 | Leukocytes | Integrin αVβ3 |
| Upregulated in Sf.Il2−/− versus Sf | |||||
| Cxcr5 | 2 | 8 | ↓ 4 | TFH | CXCL13 |
| Ccr5 | 4 | 16 | ↓ 4 | Th1 | CCL3, 4, 5, 8 |
| Ccl4 | 3 | 23 | ↓ 8 | Inflammatory T cells | CCR1, 5 |
| Ccl5 | 2 | 17 | ↓ 8 | Inflammatory T cells | CCR1, 3, 5 |
| Cd44 | 3 | 4 | ↓1.5 | Memory T cells | Hyaluronic acid |
| Upregulated in Sf and Sf.Il2−/− versus B6 | |||||
| Ccr3 | 3 | 3 | 1 | T cells, Eos, and Baso | CCL5, 7, 11, 13, 26 |
| Ccr4 | 2 | 3 | ↓ 1.5 (NS) | Th2 | CCL2, 4, 5, 17, 22 |
| Ccr6 | 2 | 5 | ↓ 2 (NS) | Th17 | CCL20 |
| Cxcr2 | 2 | 2 | 1 | Th1 (induced by IFN-γ) | CXCL1, 2, 3, 5, 8 (IL-8), |
| Cxcr3 | 3 | 4 | 1 | Th1 (induced by IFN-γ) | CXCL4, 9, 10, 11 |
| *Cxcr6 | 11 | 4 | 3 | Induced by IL-2 and IL-15 | CXCL16 |
| Cxcr7 | 2 | 3 | ↓ 1.5 (NS) | Leukocytes | CXCL11, 12 |
| *Ccl1 | 24 | 6 | 4 | T cells in asthma | CCR8 |
| Cxcl3 | 9 | 9 | 1 | Leukocytes, epithelial | CXCR2 |
| Cxcl8 | 2 | 3 | ↓ 1 (NS) | Mϕ, epithelial | CXCR1, 2 |
| Cxcl9 | 2 | 5 | ↓ 2 (NS) | Th1 (induced by IFN-γ) | CXCR3 |
| Cxcl10 | 13 | 10 | 1 | Leukocytes, endothelial | CXCR3 |
| Cxcl11 | 10 | 6 | 2 (NS) | Leukocytes, pancreas, liver | CXCR3 |
| Downregulated in Sf and Sf.Il2−/− versus B6 | |||||
| Ccr7 | ↓ 3 | ↓ 3 | 1 | LN homing | CCL19 |
| Ccl19 | ↓ 3 | ↓ 3 | 1 | LN homing | CCR7 |
| Ccl20 | ↓ 3 | ↓ 2 | ↓ 1.5 (NS) | Th17 | CCR6 |
| Itga4 | ↓ 3 | ↓ 1.5 | ↓ 2 (NS) | T cells | VCAM-1, LGALS8, paxillin |
| Itga6 | ↓ 2 | ↓ 4 | 2 | T cells | TSPAN4, GIPC1 |
| Itga9 | ↓ 5 | ↓ 2 | ↓ 2 | Leukocytes | VCAM1, cytotactin, and osteopontin |
| Itgb3 | ↓ 3 | ↓ 3 | 1 | Platelets | Fibrinogen |
| Sell | ↓ 3 | ↓2 | 1.5 (NS) | Naive T cells | Gly-CAM1, Mad-CAM1, CD34 |
| Selplg | ↓ 2 | ↓2 | 1 | Skin-homing T cells | P-selectin |
| Gene | Sf versus B6 | Sf.Il2−/− versus B6 | Sf versus Sf.Il2−/− | Cell Types | Ligand/Receptor |
|---|---|---|---|---|---|
| Upregulated in Sf versus Sf.Il2−/− | |||||
| Cysltr1 | 89 | 3 | 32 | Th2 | LTC4, LTD4, LTE4 |
| Ltb4r1 | 8 | 1 | 9 | Th2 | LTB4 |
| Il1rl1 | 12 | 1 | 14 | Th2 | IL-33 |
| Itgae | 9 | ↓ 2 | 18 | T cells | E-cadherin |
| Ccr1 | 10 | 1 | 8 | Inflammatory T cells | CCL3, 5, 7 |
| Ccr2 | 6 | 3 | 2 | Th1 | CCL2 |
| Ccr8 | 3 | 1 | 3 | T cells in asthma | CCL1 |
| Cxcr6 | 11 | 4 | 3 | Induced by IL-2 IL-15 | CXCL16 |
| Ccl1 | 24 | 6 | 4 | T cells in asthma | CCR8 |
| Ccl8 | 3 | 1.5 | 2 | Mast, Eos, Baso | CCR1, 2B, R5 |
| Cxcl2 | 4 | 1 | 4 | Monocytes, Mϕ | CXCR2 |
| Selp | 2 | 1 | 2 | Skin-homing T cells | P-selectin ligand |
| L1cam | 1 | ↓ 3 | 2 | Leukocytes | Integrin αVβ3 |
| Upregulated in Sf.Il2−/− versus Sf | |||||
| Cxcr5 | 2 | 8 | ↓ 4 | TFH | CXCL13 |
| Ccr5 | 4 | 16 | ↓ 4 | Th1 | CCL3, 4, 5, 8 |
| Ccl4 | 3 | 23 | ↓ 8 | Inflammatory T cells | CCR1, 5 |
| Ccl5 | 2 | 17 | ↓ 8 | Inflammatory T cells | CCR1, 3, 5 |
| Cd44 | 3 | 4 | ↓1.5 | Memory T cells | Hyaluronic acid |
| Upregulated in Sf and Sf.Il2−/− versus B6 | |||||
| Ccr3 | 3 | 3 | 1 | T cells, Eos, and Baso | CCL5, 7, 11, 13, 26 |
| Ccr4 | 2 | 3 | ↓ 1.5 (NS) | Th2 | CCL2, 4, 5, 17, 22 |
| Ccr6 | 2 | 5 | ↓ 2 (NS) | Th17 | CCL20 |
| Cxcr2 | 2 | 2 | 1 | Th1 (induced by IFN-γ) | CXCL1, 2, 3, 5, 8 (IL-8), |
| Cxcr3 | 3 | 4 | 1 | Th1 (induced by IFN-γ) | CXCL4, 9, 10, 11 |
| *Cxcr6 | 11 | 4 | 3 | Induced by IL-2 and IL-15 | CXCL16 |
| Cxcr7 | 2 | 3 | ↓ 1.5 (NS) | Leukocytes | CXCL11, 12 |
| *Ccl1 | 24 | 6 | 4 | T cells in asthma | CCR8 |
| Cxcl3 | 9 | 9 | 1 | Leukocytes, epithelial | CXCR2 |
| Cxcl8 | 2 | 3 | ↓ 1 (NS) | Mϕ, epithelial | CXCR1, 2 |
| Cxcl9 | 2 | 5 | ↓ 2 (NS) | Th1 (induced by IFN-γ) | CXCR3 |
| Cxcl10 | 13 | 10 | 1 | Leukocytes, endothelial | CXCR3 |
| Cxcl11 | 10 | 6 | 2 (NS) | Leukocytes, pancreas, liver | CXCR3 |
| Downregulated in Sf and Sf.Il2−/− versus B6 | |||||
| Ccr7 | ↓ 3 | ↓ 3 | 1 | LN homing | CCL19 |
| Ccl19 | ↓ 3 | ↓ 3 | 1 | LN homing | CCR7 |
| Ccl20 | ↓ 3 | ↓ 2 | ↓ 1.5 (NS) | Th17 | CCR6 |
| Itga4 | ↓ 3 | ↓ 1.5 | ↓ 2 (NS) | T cells | VCAM-1, LGALS8, paxillin |
| Itga6 | ↓ 2 | ↓ 4 | 2 | T cells | TSPAN4, GIPC1 |
| Itga9 | ↓ 5 | ↓ 2 | ↓ 2 | Leukocytes | VCAM1, cytotactin, and osteopontin |
| Itgb3 | ↓ 3 | ↓ 3 | 1 | Platelets | Fibrinogen |
| Sell | ↓ 3 | ↓2 | 1.5 (NS) | Naive T cells | Gly-CAM1, Mad-CAM1, CD34 |
| Selplg | ↓ 2 | ↓2 | 1 | Skin-homing T cells | P-selectin |
*, Upregulated both in Sf and Sf.Il2−/− versus B6 and in Sf versus Sf.Il2−/− comparisons; ↓, downregulated in Sf CD4+ T cells Sf versus Sf.Il2−/− and in Sf versus B6 comparisons; Baso, basophils; Eos, eosinophils; Mϕ, macrophages; NS, p > 0.02; TFH, follicular helper T cells; Th17, IL-17–producing T cells.
