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After the publication of this article, we were informed that a reagent used in the studies was inadvertently provided in error.
The anti-mouse IL-23p19 Ab (CNTO 6163) provided to us by Centocor was subsequently determined to actually be CNTO 3913, an Ab to mouse IL-12/23p40, which neutralizes both IL-12 and IL-23. CNTO 3913 was also intentionally utilized in the studies and appropriately noted as an anti–IL-12/23p40 Ab. We included IL-23p19 Ab results in Figs. 3, 4, and 5. Not unexpectedly, we did not find significant differences between anti–IL-23p19 and anti–IL-12/23p40 effects. However, we did state conclusions within the text that designated the pharmacological effects to IL-23. This should be corrected to more accurately describe the effects as IL-12/23p40 mediated.
Effect of anti–IL-17A, anti–IL-12/23p40, or anti–IL-23p19 on early TPA responses in K5.Stat3C mice. A, Representative histological features of TPA-treated ear skin following treatment with the indicated Abs. H&E staining. Scale bar, 100 μm. B, Epidermal ear thickness. Mean thickness ± SD (in micrometers). IgG control (n = 8), anti–IL-17A (n = 4), anti–IL-12/23p40 (n = 6), anti–IL-23p19 (n = 4). *p < 0.05, **p < 0.01, Mann–Whitney U test.
Effect of anti–IL-17A, anti–IL-12/23p40, or anti–IL-23p19 on early TPA responses in K5.Stat3C mice. A, Representative histological features of TPA-treated ear skin following treatment with the indicated Abs. H&E staining. Scale bar, 100 μm. B, Epidermal ear thickness. Mean thickness ± SD (in micrometers). IgG control (n = 8), anti–IL-17A (n = 4), anti–IL-12/23p40 (n = 6), anti–IL-23p19 (n = 4). *p < 0.05, **p < 0.01, Mann–Whitney U test.
Effect of Abs on transcriptional levels of Th17 cytokines, b-defensins, and S100As in ear skin of K5.Stat3C mice after short-term TPA treatment. Real-time RT-PCR of lesional skins revealed that anti–IL-12/23p40 (green bars) and anti–IL-23p19 (purple bars) Abs lowered transcriptional levels of Th17 cytokines, including IL-17A, IL-17F, and IL-22, b-defensin 3 (BD3), b-defensin 4 (BD4), S100A8, and S100A9, compared with the control IgG-treated group (blue bars), set to 1. Increases in Th17 cytokine mRNAs were elicited by anti–IL-17 Ab treatment (red bars) but were not statistically significant. IgG control (n = 16), anti–IL-17A (n = 8), anti–IL-12/23p40 (n = 14), anti–IL-23p19 (n = 6). *p < 0.05, **p <0.01, Kruskal–Wallis one-way analysis.
Effect of Abs on transcriptional levels of Th17 cytokines, b-defensins, and S100As in ear skin of K5.Stat3C mice after short-term TPA treatment. Real-time RT-PCR of lesional skins revealed that anti–IL-12/23p40 (green bars) and anti–IL-23p19 (purple bars) Abs lowered transcriptional levels of Th17 cytokines, including IL-17A, IL-17F, and IL-22, b-defensin 3 (BD3), b-defensin 4 (BD4), S100A8, and S100A9, compared with the control IgG-treated group (blue bars), set to 1. Increases in Th17 cytokine mRNAs were elicited by anti–IL-17 Ab treatment (red bars) but were not statistically significant. IgG control (n = 16), anti–IL-17A (n = 8), anti–IL-12/23p40 (n = 14), anti–IL-23p19 (n = 6). *p < 0.05, **p <0.01, Kruskal–Wallis one-way analysis.
Effect of Ab treatments on the development of bona fide TPA-induced psoriasiform lesions on the backs of K5.Stat3C mice. A, Representative macroscopic and histological views (H&E staining) of dorsal skins of mice treated with TPA following a single treatment with the indicated Abs. Scale bar, 200 μm. B, Epidermal thickness of the dorsal skin. Mean thickness ± SD (in micrometers). IgG control (n = 8), anti–IL-17A (n = 7), anti–IL-12/23p40 (n = 6), anti–IL-23p19 (n = 6). **p < 0.01, Mann–Whitney U-test.
Effect of Ab treatments on the development of bona fide TPA-induced psoriasiform lesions on the backs of K5.Stat3C mice. A, Representative macroscopic and histological views (H&E staining) of dorsal skins of mice treated with TPA following a single treatment with the indicated Abs. Scale bar, 200 μm. B, Epidermal thickness of the dorsal skin. Mean thickness ± SD (in micrometers). IgG control (n = 8), anti–IL-17A (n = 7), anti–IL-12/23p40 (n = 6), anti–IL-23p19 (n = 6). **p < 0.01, Mann–Whitney U-test.
To address the Ab identity error, we have reproduced the studies with the proper CNTO 6163 Ab and provide here updated Figs. 3, 4, and 5. We believe that the main conclusions of the article remain true because there were significant differences between IL-23p19 and IL-17A inhibition.
We deeply regret this inadvertent error and apologize for any inconvenience this may have caused.
