Rushworth, S. A., S. Shah, and D. J. MacEwan. 2011. TNF mediates the sustained activation of Nrf2 in human monocytes. J. Immunol. 187: 702–707.
In Fig. 4D, the incorrect immunoblot for the loading control was mistakenly used. The correct immunoblot has now been used in the updated Fig. 4. The results and the conclusions of the article remain unchanged. The corrected figure is shown below. The published figure legend is correct, but is shown below for reference. The online version of the article has been corrected and now differs from the print version as originally published.
Autocrine TNF responses regulate sustained Nrf2 activation. TNF-treated monocytes were either untreated or pretreated, or posttreated with anti-TNF, and then RNA extracted and measured for TNF expression (A). B, Nuclear extracts were prepared and separated by SDS-PAGE for Western blot analysis of Nrf2. C, Monocytes treated with TNF for 24 h were either pretreated, or posttreated with anti-TNF, and then mRNA was prepared and analyzed for GCLM, NQO1, and GSTA1. D, Monocytes were either untreated or pretreated with BAY 11-7082 for 30 min before activation with TNF for 4 and 24 h. Nuclear extracts were prepared and separated by SDS-PAGE for Western blot analysis of Nrf2, and mRNA was prepared and analyzed for NQO1 expression. Results are representative of similar findings from at least three separate experiments. Statistically significant difference of p < 0.05 exists where indicated (*).
Autocrine TNF responses regulate sustained Nrf2 activation. TNF-treated monocytes were either untreated or pretreated, or posttreated with anti-TNF, and then RNA extracted and measured for TNF expression (A). B, Nuclear extracts were prepared and separated by SDS-PAGE for Western blot analysis of Nrf2. C, Monocytes treated with TNF for 24 h were either pretreated, or posttreated with anti-TNF, and then mRNA was prepared and analyzed for GCLM, NQO1, and GSTA1. D, Monocytes were either untreated or pretreated with BAY 11-7082 for 30 min before activation with TNF for 4 and 24 h. Nuclear extracts were prepared and separated by SDS-PAGE for Western blot analysis of Nrf2, and mRNA was prepared and analyzed for NQO1 expression. Results are representative of similar findings from at least three separate experiments. Statistically significant difference of p < 0.05 exists where indicated (*).
