Brinkman, C. C., S. J. Rouhani, N. Srinivasan, and V. H. Engelhard. 2013. Peripheral tissue homing receptors enable T cell entry into lymph nodes and affect the anatomical distribution of memory cells. J. Immunol. 191: 2412–2425.

Several small errors appear in figure labeling. In Figs. 4E and 4G, the y-axis label should read “# of CD44hi CD8 Cotransfer,” not “% of CD44hi CD8 Cotransfer;” in Fig. 6E, the y-axis label should read “α4 SP of CD44hi CD8+,” not “ESL SP of CD44hi CD8+;” and in Fig. 8A, the x-axis label for the rightmost black bar should read “s.c. Vac-OVA,” not “s.v. Vac-OVA.” We apologize for any confusion this may have caused. The corrected figures are shown below. The legends were correct as published and are shown below for reference.

FIGURE 4.

α4β1 and ESL can mediate CD62L-independent entry of activated OT-I T cells into LN. (AG) CD8 T cells were enriched from LN and spleen of s.c. BMDC immunized mice, labeled with CellTrace Violet, treated with MEL-14 for 30 min and injected into naive B6 mice. Recipients were treated i.p. with 100 μg 9A9, RB40, MK2-7, or Rat IgG control Abs 8 h prior to T cell transfer, and processed for flow cytometry 16 h later. Skin LN composed of pooled axillary, brachial, cervical, and inguinal LN. (A and B) Results are from at least three mice per group from one experiment representative of two independent experiments. (C) Plots are gated on labeled Thy1.1+CD8+ OT-I or Thy1.1negCD44+CD8+ endogenous T cells and positive staining thresholds were set with FMO controls. (C–G) Results are from at least three mice per group from one experiment representative of three independent experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 unpaired Student t test in (A) and (B), Tukey posttests of one-way ANOVA in (D–G).

FIGURE 4.

α4β1 and ESL can mediate CD62L-independent entry of activated OT-I T cells into LN. (AG) CD8 T cells were enriched from LN and spleen of s.c. BMDC immunized mice, labeled with CellTrace Violet, treated with MEL-14 for 30 min and injected into naive B6 mice. Recipients were treated i.p. with 100 μg 9A9, RB40, MK2-7, or Rat IgG control Abs 8 h prior to T cell transfer, and processed for flow cytometry 16 h later. Skin LN composed of pooled axillary, brachial, cervical, and inguinal LN. (A and B) Results are from at least three mice per group from one experiment representative of two independent experiments. (C) Plots are gated on labeled Thy1.1+CD8+ OT-I or Thy1.1negCD44+CD8+ endogenous T cells and positive staining thresholds were set with FMO controls. (C–G) Results are from at least three mice per group from one experiment representative of three independent experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 unpaired Student t test in (A) and (B), Tukey posttests of one-way ANOVA in (D–G).

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FIGURE 6.

Expression of peripheral tissue HR influences the distribution of LN-resident memory cells independent of CD62L Thy 1.2+ mice received CFSE+Thy 1.1+ OT-I cells and were immunized i.v. (A and F) or s.c. (B–F) with BMDC. Mice were harvested 6–8 wk later. Plots are gated on CD44hiCD8+ Thy1.1+ cells in (A) and (B) and positive staining thresholds were set with FMO controls. (D and E) CD44hiCD8+Thy1.1neg (non OT-I) T cells were gated on and the fraction expressing the indicated peripheral tissue HR was determined. Skin LN composed of pooled axillary, brachial, cervical, and inguinal LN. (A and B) Results representative of 6–10 mice of each condition from three to four experiments. (C) Results are pooled data of five to six mice from two experiments. (D and E) Results are pooled data of eight mice from two experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 paired Student t test compared with skin LN in (C–E), unpaired in (F). In (F), total skin T cell cellularity estimated by extrapolating cell counts from two ears (∼2 cm2) to the entire surface area of a 20-g mouse (∼36 cm2).

FIGURE 6.

Expression of peripheral tissue HR influences the distribution of LN-resident memory cells independent of CD62L Thy 1.2+ mice received CFSE+Thy 1.1+ OT-I cells and were immunized i.v. (A and F) or s.c. (B–F) with BMDC. Mice were harvested 6–8 wk later. Plots are gated on CD44hiCD8+ Thy1.1+ cells in (A) and (B) and positive staining thresholds were set with FMO controls. (D and E) CD44hiCD8+Thy1.1neg (non OT-I) T cells were gated on and the fraction expressing the indicated peripheral tissue HR was determined. Skin LN composed of pooled axillary, brachial, cervical, and inguinal LN. (A and B) Results representative of 6–10 mice of each condition from three to four experiments. (C) Results are pooled data of five to six mice from two experiments. (D and E) Results are pooled data of eight mice from two experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 paired Student t test compared with skin LN in (C–E), unpaired in (F). In (F), total skin T cell cellularity estimated by extrapolating cell counts from two ears (∼2 cm2) to the entire surface area of a 20-g mouse (∼36 cm2).

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FIGURE 8.

Influence of tissue-homing molecule expression on the recall response. LN and spleen from mice that had received Thy1.1+ OT-I cells were harvested 28 d after s.c. or i.v. BMDC immunization. Cells were flow sorted to select for CD44hi CD8+ Thy1.1+ cells. α4+ESLneg and α4negESL+ subsets were collected separately. A total of 1 × 104 sorted cells were transferred into naive Thy1.2+ mice together with 3 × 106 CFSE+ B6 splenocytes. These were used as an internal control for differences in injection efficiency and cellular distribution among mice. Five days later, mice were challenged with 1 × 106 PFU vac-OVA i.v. or s.c. or left unchallenged. (A and B) Summary data from rechallenged mice. (C) Summary of CD62L expression by α4 SP and ESL SP OT-I from mice immunized i.v. or s.c. with BMDC. Skin LN comprised of pooled axillary, brachial, cervical, and inguinal LN. Column labels indicate sorted populations and row labels indicate route of vac-OVA and site of analysis. (D) Representative two-dimensional FACS plots from rechallenged mice gated on divided CD44hiCD8+Thy1.1+ cells and positive staining thresholds were set with FMO controls. (A, B, C, E and F) Pooled data from four to eight mice per group from two experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student t test.

FIGURE 8.

Influence of tissue-homing molecule expression on the recall response. LN and spleen from mice that had received Thy1.1+ OT-I cells were harvested 28 d after s.c. or i.v. BMDC immunization. Cells were flow sorted to select for CD44hi CD8+ Thy1.1+ cells. α4+ESLneg and α4negESL+ subsets were collected separately. A total of 1 × 104 sorted cells were transferred into naive Thy1.2+ mice together with 3 × 106 CFSE+ B6 splenocytes. These were used as an internal control for differences in injection efficiency and cellular distribution among mice. Five days later, mice were challenged with 1 × 106 PFU vac-OVA i.v. or s.c. or left unchallenged. (A and B) Summary data from rechallenged mice. (C) Summary of CD62L expression by α4 SP and ESL SP OT-I from mice immunized i.v. or s.c. with BMDC. Skin LN comprised of pooled axillary, brachial, cervical, and inguinal LN. Column labels indicate sorted populations and row labels indicate route of vac-OVA and site of analysis. (D) Representative two-dimensional FACS plots from rechallenged mice gated on divided CD44hiCD8+Thy1.1+ cells and positive staining thresholds were set with FMO controls. (A, B, C, E and F) Pooled data from four to eight mice per group from two experiments. Bars show mean values (± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student t test.

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