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In the version of this article originally published, incorrect versions of Fig. 3F and Supplemental Fig. 3B, without data from Ripk1DC KOMlkl/− and littermate controls, were inadvertently included. A revised version of Fig. 3 is shown below. The legend for Fig. 3 was correct as published and is shown below for reference.

FIGURE 3.

DC necroptosis underlies the systemic inflammation in Ripk1DC KO mice. (A) DCs from CD11cCre Ripk1fl/D138N (Ripk1DC KO/D138N) and Ripk1DC KORipk3−/− mice are protected from necroptosis in vitro. BMDCs were treated with SMAC mimetics and zVAD-fmk. Effects on cell viability were measured at 18 h using Cell Titer Glo. n = 3–6 samples. (B) Spleen and lymph node (LN) weights and lymph node cellularity from CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (C) Neutrophil and inflammatory monocyte numbers in the spleens of CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (D) H&E and Masson’s trichrome staining of spleens from Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (E) Neutrophils, inflammatory monocytes, and B cells are elevated in the lymph nodes of Ripk1DC KO and Ripk1DC KORipk3−/− mice but not Ripk1DC KO/D138N or Ripk1DC KOMlkl−/− mice. (F) Proportion of mice serum positive for ANAs at 6 mo (n = 3–6 mice per genotype). Unless otherwise stated, all data shown are from 16 wk-old mice. CD11cCre and Ripk1DC KO mouse phenotyping data shown in (B), (C), (E), and (F) were shown in Fig. 2. Error bars, mean ± SEM. Scale bars, 100 μm. Unpaired two-tailed Student t test (A–C and E). *p < 0.05, **p < 0.01.

FIGURE 3.

DC necroptosis underlies the systemic inflammation in Ripk1DC KO mice. (A) DCs from CD11cCre Ripk1fl/D138N (Ripk1DC KO/D138N) and Ripk1DC KORipk3−/− mice are protected from necroptosis in vitro. BMDCs were treated with SMAC mimetics and zVAD-fmk. Effects on cell viability were measured at 18 h using Cell Titer Glo. n = 3–6 samples. (B) Spleen and lymph node (LN) weights and lymph node cellularity from CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (C) Neutrophil and inflammatory monocyte numbers in the spleens of CD11cCre, Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (D) H&E and Masson’s trichrome staining of spleens from Ripk1DC KO, Ripk1DC KO/D138N, Ripk1DC KORipk3−/−, and Ripk1DC KOMlkl−/− mice. (E) Neutrophils, inflammatory monocytes, and B cells are elevated in the lymph nodes of Ripk1DC KO and Ripk1DC KORipk3−/− mice but not Ripk1DC KO/D138N or Ripk1DC KOMlkl−/− mice. (F) Proportion of mice serum positive for ANAs at 6 mo (n = 3–6 mice per genotype). Unless otherwise stated, all data shown are from 16 wk-old mice. CD11cCre and Ripk1DC KO mouse phenotyping data shown in (B), (C), (E), and (F) were shown in Fig. 2. Error bars, mean ± SEM. Scale bars, 100 μm. Unpaired two-tailed Student t test (A–C and E). *p < 0.05, **p < 0.01.

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Supplemental Fig. 3B has been updated in the supplemental material online. Fig. 3 has also been corrected in the online version of the article, which now differs from the print version as originally published.