Zheng, H., J. Wang, B. Li, L. Guo, H. Li, J. Song, Z. Yang, H. Li, H. Fan, X. Huang, H. Long, C. Cheng, M. Chu, Z. He, W. Yu, J. Li, Y. Gao, R. Ning, N. Li, J. Yang, Q. Wu, H. Shi, M. Sun and L. Liu. 2018. A novel neutralizing antibody specific to the DE loop of VP1 can inhibit EV-D68 infection in mice. J. Immunol. 201: 2557–2569.
In Fig. 1E and Fig. 1F, the plots in the second column were incorrect. The correct plots for the second column should have shown the double positive, CD20+CD27+. The correct x-axis label of the second column should be CD20, and the y-axis should be CD27. The corrected versions of Fig. 1E and Fig. 1F are shown below. The legend for Fig. 1 was correct as published and is shown below for reference.
The establishment of an EV-D68–infected rhesus monkey model and single memory B cell sorting. Dynamic profiles of EV-D68 in (A) the blood and (B) the nasal swabs and feces of EV-D68–infected rhesus monkeys. EV-D68 viral RNA extracted from these samples was detected using TaqMan real-time quantitative PCR. The dotted line represents the SEM of viral load changes in feces; the solid line represents the SEM of viral load changes in nasal swab. (C) Neutralizing Ab responses to EV-D68 virus after 0, 7, 14, and 28 dpi. The neutralization Ab titer was the reciprocal of the highest serum dilution that inhibited 50% of the viral CPE. (D) Reactivity of the anti–EV-D68 sera from the infected monkeys against the EV-D68 VP1 protein after 0, 7, 14, and 28 dpi; the absorbance value was measured at 450 nm. The results presented are representative of three independent experiments. (E and F) Single-cell sorting of EV-D68 VP1 memory B cells by flow cytometry. The numbers in the density plots indicate the percentages of cells gated from the previous gate side scatter (SSC).
The establishment of an EV-D68–infected rhesus monkey model and single memory B cell sorting. Dynamic profiles of EV-D68 in (A) the blood and (B) the nasal swabs and feces of EV-D68–infected rhesus monkeys. EV-D68 viral RNA extracted from these samples was detected using TaqMan real-time quantitative PCR. The dotted line represents the SEM of viral load changes in feces; the solid line represents the SEM of viral load changes in nasal swab. (C) Neutralizing Ab responses to EV-D68 virus after 0, 7, 14, and 28 dpi. The neutralization Ab titer was the reciprocal of the highest serum dilution that inhibited 50% of the viral CPE. (D) Reactivity of the anti–EV-D68 sera from the infected monkeys against the EV-D68 VP1 protein after 0, 7, 14, and 28 dpi; the absorbance value was measured at 450 nm. The results presented are representative of three independent experiments. (E and F) Single-cell sorting of EV-D68 VP1 memory B cells by flow cytometry. The numbers in the density plots indicate the percentages of cells gated from the previous gate side scatter (SSC).
