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The HVEM and IFN-γ blots of Fig. 2C and the HSP70 vac/pcDNA3.1 panel of Fig. 5 were incorrect in the published article. In both figures, incorrect images were inadvertently pasted into the file during preparation of the figures. The corrected figures are shown below. The figure legends were correct as published and are shown below for reference. The authors sincerely apologize for these mistakes in the article and are very sorry for any inconvenience.
Antitumor effects of sBTLA by local gene transfection. A, Expression of sBTLA in muscle tissue after psBTLA local gene transfection. Naked plasmid DNA was injected into mice i.m. (n = 3 per group), and mRNA and protein levels were determined by RT-PCR and Western blot, respectively, 72 h after transfection. B, Inhibitory effects of in vivo transfection of psBTLA on tumor growth. Mice (n = 5 per group at each time point) were inoculated with TC-1 cells and treated with psBTLA (four times for 14 days and eight times for 28 days) as described in Materials and Methods. C, Effects of sBTLA on immune effects in local tumor tissues. Mice were inoculated with TC-1 cells and treated with saline, pcDNA3.1, or psBTLA. On days 14 and 28 after tumor inoculation, total RNA was isolated from tumor marginal tissues of three mice in each group at each time point, and the transcription levels of BTLA, HVEM, IL-2, IFN-γ, IL-10, and TGF-β were analyzed by RT-PCR and real-time PCR. D, Microscopic findings of TILs with different treatments. Tumor tissues on day 14 after tumor inoculation were sliced and stained with H&E staining. Images are representative of multiple microscopic fields observed (×200) in three mice per group. Data are representative of two independent experiments.
Antitumor effects of sBTLA by local gene transfection. A, Expression of sBTLA in muscle tissue after psBTLA local gene transfection. Naked plasmid DNA was injected into mice i.m. (n = 3 per group), and mRNA and protein levels were determined by RT-PCR and Western blot, respectively, 72 h after transfection. B, Inhibitory effects of in vivo transfection of psBTLA on tumor growth. Mice (n = 5 per group at each time point) were inoculated with TC-1 cells and treated with psBTLA (four times for 14 days and eight times for 28 days) as described in Materials and Methods. C, Effects of sBTLA on immune effects in local tumor tissues. Mice were inoculated with TC-1 cells and treated with saline, pcDNA3.1, or psBTLA. On days 14 and 28 after tumor inoculation, total RNA was isolated from tumor marginal tissues of three mice in each group at each time point, and the transcription levels of BTLA, HVEM, IL-2, IFN-γ, IL-10, and TGF-β were analyzed by RT-PCR and real-time PCR. D, Microscopic findings of TILs with different treatments. Tumor tissues on day 14 after tumor inoculation were sliced and stained with H&E staining. Images are representative of multiple microscopic fields observed (×200) in three mice per group. Data are representative of two independent experiments.
HSP70 vaccine combined with sBTLA increases infiltration of CD8+ T cells in tumor tissue. The tissues were removed from tumor marginal area at day 28 in different treatment groups (n = 5 per group) and stained with anti-mouse CD8 mAb. The number of CD8+ T cells was counted from five high-power fields (×200) in three mice per group.
HSP70 vaccine combined with sBTLA increases infiltration of CD8+ T cells in tumor tissue. The tissues were removed from tumor marginal area at day 28 in different treatment groups (n = 5 per group) and stained with anti-mouse CD8 mAb. The number of CD8+ T cells was counted from five high-power fields (×200) in three mice per group.
