Wang, Z., L. Kong, S. Tan, Y. Zhang, X. Song, T. Wang, Q. Lin, Z. Wu, P. Xiang, C. Li, L. Gao, X. Liang, and C. Ma. 2020. Zhx2 accelerates sepsis by promoting macrophage glycolysis via Pfkfb3. J. Immunol. 204: 2232–2241.

The n error values were omitted from the legend for (Fig. 1C and (1G. The corrected legend for (Fig. 1 is shown below.

FIGURE 1.

Zhx2 protects mice from sepsis shock and inhibits inflammation. (A) C57BL/6 mice were challenged with PBS and LPS (10 mg/kg, i.p.) for 24 h. CD8 T cell (CD3+ CD8+), B cell (CD3 CD19+), macrophage (CD11b+ F4/80+), and dendritic cell (CD11b+CD11c+) from spleen were sorted by MoFlo Astrios EQ. Zhx2 mRNA was detected by RT-PCR. (B) BMDM from WT C57BL/6 mice were treated with 100 ng/ml LPS for the indicated duration of time. Levels of Zhx2 were determined by RT-PCR. (C–J) WT and MKO mice challenged with LPS (10 mg/kg, i.p.) (C–F) or CLP treatment (G–J) to induce sepsis. (C and G) Survival curves (PBS: n/WT = 10, n/MKO = 10, LPS: n/WT = 26, n/MKO = 25; sham operation (abbreviated as Sham): n/WT = 9, n/MKO = 9, CLP: n/WT = 14, n/MKO = 15), ****p value for four groups and *p value for LPS or CLP-treated WT/MKO mice. (D and H) H&E staining of lung tissues. Original magnification ×100. Scale bars, 20 μm. (E and I) Serum concentrations of IL-6, TNF-α, and IL-1 β after 24 h (n = 4). (F and J) Serum concentrations of lactate (n = 6) were analyzed. Data were presented as mean ± SD from three independent experiments and analyzed by unpaired, two-tailed t test with significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

FIGURE 1.

Zhx2 protects mice from sepsis shock and inhibits inflammation. (A) C57BL/6 mice were challenged with PBS and LPS (10 mg/kg, i.p.) for 24 h. CD8 T cell (CD3+ CD8+), B cell (CD3 CD19+), macrophage (CD11b+ F4/80+), and dendritic cell (CD11b+CD11c+) from spleen were sorted by MoFlo Astrios EQ. Zhx2 mRNA was detected by RT-PCR. (B) BMDM from WT C57BL/6 mice were treated with 100 ng/ml LPS for the indicated duration of time. Levels of Zhx2 were determined by RT-PCR. (C–J) WT and MKO mice challenged with LPS (10 mg/kg, i.p.) (C–F) or CLP treatment (G–J) to induce sepsis. (C and G) Survival curves (PBS: n/WT = 10, n/MKO = 10, LPS: n/WT = 26, n/MKO = 25; sham operation (abbreviated as Sham): n/WT = 9, n/MKO = 9, CLP: n/WT = 14, n/MKO = 15), ****p value for four groups and *p value for LPS or CLP-treated WT/MKO mice. (D and H) H&E staining of lung tissues. Original magnification ×100. Scale bars, 20 μm. (E and I) Serum concentrations of IL-6, TNF-α, and IL-1 β after 24 h (n = 4). (F and J) Serum concentrations of lactate (n = 6) were analyzed. Data were presented as mean ± SD from three independent experiments and analyzed by unpaired, two-tailed t test with significance. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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In addition, because of an error in the assembly of (Fig. 2A, the flow cytometry result for the WT IL-6 group was mistakenly duplicated from that of the MKO TNF-α group. This inadvertent error does not influence the interpretations and conclusions for (Fig. 2 and for the work as a whole. The corrected version of (Fig. 2 is shown below. The figure legend was correct as published and is shown below for reference.

FIGURE 2.

Zhx2 aggravates proinflammatory factor secretion from macrophages in septic mice. WT and MKO mice were injected with i.p. LPS (10 mg/kg; n = 4). (A and B) Gated on CD11b+F4/80+ cells, intracellular TNF-α, IL-6, and IL-1β were detected by flow cytometry. (A) Representative dot plots were shown on the left panels and the percentage (top) and number (bottom) were shown on the right panels. (B) Representative histograms and mean fluorescence intensities (MFIs) were shown. (C) F4/80+ macrophages were isolated from the spleen of WT and MKO mice. RT-PCR detected the mRNA expression of TNF-α, IL-6, and IL-1β (n = 6). (D) PM was harvested from WT and MKO mice challenged with LPS for 24 h. Western blot detected the protein level of IL-6 and IL-1β. The data were presented as means ± SD from two independent experiments and analyzed by unpaired, two-tailed t test with significance. *p < 0.05, **p < 0.01, ***p < 0.001.

FIGURE 2.

Zhx2 aggravates proinflammatory factor secretion from macrophages in septic mice. WT and MKO mice were injected with i.p. LPS (10 mg/kg; n = 4). (A and B) Gated on CD11b+F4/80+ cells, intracellular TNF-α, IL-6, and IL-1β were detected by flow cytometry. (A) Representative dot plots were shown on the left panels and the percentage (top) and number (bottom) were shown on the right panels. (B) Representative histograms and mean fluorescence intensities (MFIs) were shown. (C) F4/80+ macrophages were isolated from the spleen of WT and MKO mice. RT-PCR detected the mRNA expression of TNF-α, IL-6, and IL-1β (n = 6). (D) PM was harvested from WT and MKO mice challenged with LPS for 24 h. Western blot detected the protein level of IL-6 and IL-1β. The data were presented as means ± SD from two independent experiments and analyzed by unpaired, two-tailed t test with significance. *p < 0.05, **p < 0.01, ***p < 0.001.

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The online version of the article has been corrected and now differs from the print version as originally published.