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Fig. 5D was incorrect as originally published. Although the means should have been presented as horizontal bars as in other figures, they were presented as dots only in Fig. 5D. The dots at the center of the error bars have been changed into horizontal bars in Fig. 5D. The corrected version of Fig. 5 is shown below. This correction does not affect the statistical analysis and conclusions. The figure legend was correct as published and is shown below for reference. The figure has been corrected in the online version of the article, which now differs from the print version as originally published.

FIGURE 5.

Intracellular IL-17A staining and flow cytometric analysis of lung cells collected from WT mice 2 h after intranasal PDE administration. (A) Representative gating strategy of IL-17A+ γδ T cells, CD4+ cells, and neutrophils. (B) The percentage of IL-17A+ cells in each cell population. (C) The total cell counts of γδ T cells and CD4+ cells. (D) IL-17A+ cell counts in each cell population. The data are presented as the means ± SEM and as dot plots for each group (n = 3–5 for γδ T cells and CD4+ cells, n = 1–2 for neutrophils). Except for neutrophils, the data are representative of two to three experiments. *p < 0.05. (E) Representative gating strategies of Vγ1+, Vγ4+, Vγ1Vγ4 cells on CD3+TCRγδ+ cells at day 8. (F) The numbers of total cells and IL-17A+ cells in Vγ1+, Vγ4+, Vγ1Vγ4 cells from day 1 to day 22 in the lungs of PDE or control mice. The data are presented as the mean ± SEM (n = 3). *p < 0.05. (G) Representative gating strategies of CD27+ or CD44+ cells on CD3+TCRγδ+ cells at day 8. (H) The numbers of CD27+ or CD44+ γδ T cells and the ratio of CD27+/CD44+ from day 1 to day 22 in PDE or control mice. The data are presented as the mean ± SEM (n = 3). *p < 0.05. (I) Immunofluorescence staining of TCRγδ (light blue), IL-17A (red), and DAPI (blue) in the lungs at WK1 and WK3. These are representative of four to five granulomatous or cell-infiltrated areas from three mice.

FIGURE 5.

Intracellular IL-17A staining and flow cytometric analysis of lung cells collected from WT mice 2 h after intranasal PDE administration. (A) Representative gating strategy of IL-17A+ γδ T cells, CD4+ cells, and neutrophils. (B) The percentage of IL-17A+ cells in each cell population. (C) The total cell counts of γδ T cells and CD4+ cells. (D) IL-17A+ cell counts in each cell population. The data are presented as the means ± SEM and as dot plots for each group (n = 3–5 for γδ T cells and CD4+ cells, n = 1–2 for neutrophils). Except for neutrophils, the data are representative of two to three experiments. *p < 0.05. (E) Representative gating strategies of Vγ1+, Vγ4+, Vγ1Vγ4 cells on CD3+TCRγδ+ cells at day 8. (F) The numbers of total cells and IL-17A+ cells in Vγ1+, Vγ4+, Vγ1Vγ4 cells from day 1 to day 22 in the lungs of PDE or control mice. The data are presented as the mean ± SEM (n = 3). *p < 0.05. (G) Representative gating strategies of CD27+ or CD44+ cells on CD3+TCRγδ+ cells at day 8. (H) The numbers of CD27+ or CD44+ γδ T cells and the ratio of CD27+/CD44+ from day 1 to day 22 in PDE or control mice. The data are presented as the mean ± SEM (n = 3). *p < 0.05. (I) Immunofluorescence staining of TCRγδ (light blue), IL-17A (red), and DAPI (blue) in the lungs at WK1 and WK3. These are representative of four to five granulomatous or cell-infiltrated areas from three mice.

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