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In the original version of this article, the immunohistochemical (IHC) staining of IL-6 in Fig. 6D was incorrectly labeled as IL-1. In addition, the IHC staining of IL-6 of wild-type piglets in this panel was mistakenly a duplicate of the IL-1β of wild-type piglets. The corrected version of Fig. 6 is shown below. The figure has been corrected in the online version of the article, which now differs from the print version as originally published. The figure legend was correct as published and is shown below for reference.
NLRP3 R259W homo adult pigs exhibited more intense inflammation than that in wild-type pigs after delayed-type contact hypersensitivity induced with DNCB. (A) Photos of NLRP3 R259W homo and age-matched wild-type pigs and after application of DNCB solution to the back for 0, 1, 2, 3, 4, 5, and 6 d. The NLRP3 R259W homo pigs showed more contraction and redness in the back skin than the control pigs. (B) Ears of NLRP3 R259W homo and age-matched control pigs stimulated by 0.5% DNCB. (C) H&E staining of ear tissues from NLRP3 R259W homo and wild-type pigs that developed inflammation upon DTH induction with DNCB. Top, the ear epidermis of NLRP3 R259W homo and wild-type pigs thickens because of cellular infiltration. Scale bar, 1000 μm. Bottom, high magnification of the selected region from the top panel shows that the NLRP3 R259W homo tissue is more infiltrated by neutrophils. Scale bar, 100 μm. (D) IHC staining for the analysis of the expression levels of NLRP3, Caspase-1, IL-1β, TNF-α, IL-6, and IL-17 in the stimulated ears of NLRP3 R259W homo and wild-type pigs. Scale bar, 200 μm. (E) Q-PCR analysis for indicated cytokines in the stimulated ears of NLRP3 R259W homo and wild-type pigs. Asterisk indicates statistical significance in comparison with wild-type as follows. *p < 0.05, **p < 0.01. n.s, not significant.
NLRP3 R259W homo adult pigs exhibited more intense inflammation than that in wild-type pigs after delayed-type contact hypersensitivity induced with DNCB. (A) Photos of NLRP3 R259W homo and age-matched wild-type pigs and after application of DNCB solution to the back for 0, 1, 2, 3, 4, 5, and 6 d. The NLRP3 R259W homo pigs showed more contraction and redness in the back skin than the control pigs. (B) Ears of NLRP3 R259W homo and age-matched control pigs stimulated by 0.5% DNCB. (C) H&E staining of ear tissues from NLRP3 R259W homo and wild-type pigs that developed inflammation upon DTH induction with DNCB. Top, the ear epidermis of NLRP3 R259W homo and wild-type pigs thickens because of cellular infiltration. Scale bar, 1000 μm. Bottom, high magnification of the selected region from the top panel shows that the NLRP3 R259W homo tissue is more infiltrated by neutrophils. Scale bar, 100 μm. (D) IHC staining for the analysis of the expression levels of NLRP3, Caspase-1, IL-1β, TNF-α, IL-6, and IL-17 in the stimulated ears of NLRP3 R259W homo and wild-type pigs. Scale bar, 200 μm. (E) Q-PCR analysis for indicated cytokines in the stimulated ears of NLRP3 R259W homo and wild-type pigs. Asterisk indicates statistical significance in comparison with wild-type as follows. *p < 0.05, **p < 0.01. n.s, not significant.
