Mellanby, R. J., C. H. Koonce, A. Monti, J. M. Phillips, A. Cooke, and E. K. Bikoff. 2006. Loss of invariant chain protects nonobese diabetic mice against type 1 diabetes. J. Immunol. 177: 7588–7598.
In the original Fig. 3, a figure setting mistake resulted in a duplication of an image in the right panel of Fig. 3A. In the revised figure, the authors have inserted the correct FACS plot with an unaltered gating and percentage in the top right panel of Figure 3A. The figure legend was correct as published and is shown below.
Presentation of islet Ags by Ii chain-deficient dendritic cells (DCs). A, CD11c+ splenic DCs from age-matched wild-type or Ii chain NOD mutants were incubated for 1 h with cognate peptide ligand, washed, and then cultured for 72 h with CFSE-labeled BDC2.5NOD T cells at various ratios as indicated. Representative profiles gated on Vβ4-positive cells from triplicate wells are shown. B, CD11c+ splenic DCs (1 × 104) were incubated for 3 h with macerated islets, subsequently washed, and then cultured for 72 h with 1 × 105 CFSE-labeled BDC2.5 NOD T cells. Wild-type and Ii chain mutant DCs display equivalent Ag presentation capabilities in the presence of peptide or intact islets.
Presentation of islet Ags by Ii chain-deficient dendritic cells (DCs). A, CD11c+ splenic DCs from age-matched wild-type or Ii chain NOD mutants were incubated for 1 h with cognate peptide ligand, washed, and then cultured for 72 h with CFSE-labeled BDC2.5NOD T cells at various ratios as indicated. Representative profiles gated on Vβ4-positive cells from triplicate wells are shown. B, CD11c+ splenic DCs (1 × 104) were incubated for 3 h with macerated islets, subsequently washed, and then cultured for 72 h with 1 × 105 CFSE-labeled BDC2.5 NOD T cells. Wild-type and Ii chain mutant DCs display equivalent Ag presentation capabilities in the presence of peptide or intact islets.
