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In the original article, Fig. 5E had inadvertently misplaced overlapping TIRAP−/− and TIRAP+/+ images in unstimulated and TNF-α–stimulated panels. The version of the figure shown below includes the correct TIRAP+/+ images in unstimulated and TNF-α–stimulated panels from the original data in Fig. 5E. The figure legend was correct as published and is shown below for reference.

FIGURE 5.

(A and B) Unimpaired capacity of TIRAP−/− and TIRAP+/+ neutrophils to uptake/kill Kp. A total of 106 CFU of Kp was cultured alone or in the presence of 106 CFU of bone marrow-derived polymorphonuclear neutrophils from TIRAP−/− or TIRAP+/+ mice in 1 ml of medium. Viable bacteria were then enumerated at 2 and 6 h after culture. The CFU values shown represent mean ± SE of a single experiment performed in triplicate from three mice per group. (C and D) Impaired up-regulation of VCAM-1 in the lung after Kp but not Pa inoculation. The lungs were homogenized, total protein in the lungs was quantitated, and proteins were resolved on an 8–15% gel. The autoradiograph of VCAM-1 is a representative blot of six mice from three separate experiments (C). The graph shows VCAM-1 (D) for each sample expressed relative to the densitometric value of saline-treated sample at 24 h. *, p < 0.05; significant differences between TIRAP−/− and TIRAP+/+ mice. (E) Unaltered actin assembly in TIRAP−/− and TIRAP+/+ neutrophils after chemokine and cytokine stimulation. Bone marrow-derived neutrophils were isolated and stimulated with MIP-2 and TNF-α. Cells were labeled with nitrobenzoxadiazole-phallacidin, and actin cytoskeleton changes were assessed by an immunofluorescence microscope. Photographs are representative of the pictures of cells from two separate experiments (five mice in each group) with identical results.

FIGURE 5.

(A and B) Unimpaired capacity of TIRAP−/− and TIRAP+/+ neutrophils to uptake/kill Kp. A total of 106 CFU of Kp was cultured alone or in the presence of 106 CFU of bone marrow-derived polymorphonuclear neutrophils from TIRAP−/− or TIRAP+/+ mice in 1 ml of medium. Viable bacteria were then enumerated at 2 and 6 h after culture. The CFU values shown represent mean ± SE of a single experiment performed in triplicate from three mice per group. (C and D) Impaired up-regulation of VCAM-1 in the lung after Kp but not Pa inoculation. The lungs were homogenized, total protein in the lungs was quantitated, and proteins were resolved on an 8–15% gel. The autoradiograph of VCAM-1 is a representative blot of six mice from three separate experiments (C). The graph shows VCAM-1 (D) for each sample expressed relative to the densitometric value of saline-treated sample at 24 h. *, p < 0.05; significant differences between TIRAP−/− and TIRAP+/+ mice. (E) Unaltered actin assembly in TIRAP−/− and TIRAP+/+ neutrophils after chemokine and cytokine stimulation. Bone marrow-derived neutrophils were isolated and stimulated with MIP-2 and TNF-α. Cells were labeled with nitrobenzoxadiazole-phallacidin, and actin cytoskeleton changes were assessed by an immunofluorescence microscope. Photographs are representative of the pictures of cells from two separate experiments (five mice in each group) with identical results.

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