In a previous paper Suzuki (1) has shown the value of the tissue culture as a means for a more detailed study of the nature of the conditions of natural immunity in animals. The present paper describes other experiments outlined for the purpose of studying more carefully the general distribution and action of antitoxic substances. It has been shown by Suzuki that chickens' tissues are sensitive in the tissue culture to the action of diphtheria toxin when this is added to the medium. Antitoxin when injected into chickens protects the chickens against lethal doses of toxin. The distribution of the antitoxin within these animals became of interest for analysis. Again, it seemed evident that the tissue culture method would be of use for the quantitative standardization of toxins. It thus became of interest to test the neutralizing power of antitoxin for toxin by this method.

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