From these studies of vaccinial systems a short quantitative method of titrating the complement-fixing capacity of vaccinial sera and antigens has been derived. The method is based on that developed in this laboratory (5) for the routine complement-fixation test for syphilis, and involves the use of tables of conversion factors for the determination of the amount of complement needed for 50-per-cent hemolysis under various conditions. The test has been useful in differentiating reactions of sera with several different antigens and of antigens with different sera.

1

The author is very grateful to Dr. W. R. Thompson for advice in regard to the mathematical discussion in the paper.

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Copyright © 1946 by The American Association of Immunologists, Inc.
1946
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